Microbiology Spectrum,
Journal Year:
2023,
Volume and Issue:
11(6)
Published: Oct. 31, 2023
The
role
of
the
upper
respiratory
tract
(URT)
microbiome
in
predicting
lung
health
has
been
documented
several
studies.
dysbiosis
COVID
patients
associated
with
disease
outcomes
by
modulating
host
immune
system.
However,
although
it
known
that
different
SARS-CoV-2
variants
manifest
distinct
transmissibility
and
mortality
rates
human
populations,
their
effect
on
composition
diversity
URT
not
studied
to
date.
Unlike
older
variant
(Delta),
newer
(Omicron)
have
become
more
transmissible
lesser
symptoms
also
changed
significantly.
Hence,
present
study,
we
investigated
change
Delta
Omicron
identified
variant-specific
signatures
will
be
useful
assessment
can
utilized
for
nasal
probiotic
therapy
future.
EFSA Supporting Publications,
Journal Year:
2024,
Volume and Issue:
21(2)
Published: Feb. 1, 2024
Scientific
interest
in
the
use
of
environmental
microbiomes
for
risk
assessment
is
rapidly
growing,
as
exemplified
by
various
EFSA
opinions.
In
absence
official
regulatory
guidelines
on
how
to
integrate
assessment,
aims
this
report
are
therefore
determine
whether
microbiome
studies
can
be
used
such
purposes,
and
propose
a
roadmap
integration
assessments
under
EFSA's
remit.
The
identifies
current
gaps
(in
terms
knowledge
from
technical
point
view)
barriers
that
might
delay
implementation
methods,
offers
recommendations
standardised
(multi-)omics
techniques
purposes.
Our
main
findings
identified
five
priorities:
(i)
defining
core
(what
it
encompasses
what
made
of,
including
identification
bioindicators)
assess
impact
any
type
disturbance;
(ii)
standardising
methodologies
protocols,
sampling
interpretation,
guarantee
comparability
analyses;
(iii)
developing
tools
facilitate
interpretation;
(iv)
collecting
microbiome-based
data
shared,
curated
maintained
databases;
(v)
setting
up
European
Network
Microbiome
Laboratories
reach
an
agreement
standardise
studies,
interactions
between
researchers
access
or
samples,
actively
include
multiple
stakeholders
discussions
involving
assessment.
There
both
short-
longer-term
priorities,
all
which
highlight
need
mobilise
concurrently
different
agencies
institutions,
well
research.
also
points
out
capacity
building
training,
acceptance
emerging
technology,
communication
issues.
These
will
hopefully
contribute
elaboration
widely
accepted
framework
dealing
with.
Animal Microbiome,
Journal Year:
2023,
Volume and Issue:
5(1)
Published: Oct. 24, 2023
Microbiome
in
early
life
has
long-term
effects
on
the
host's
immunological
and
physiological
development
its
disturbance
is
known
to
trigger
various
diseases
host
Deuterostome
animals.
The
sea
cucumber
Apostichopus
japonicus
one
of
most
valuable
marine
invertebrates
Asia
a
model
animal
regeneration
studies.
To
understand
factors
that
impact
holobiont
maintenance,
host-microbiome
association
been
actively
studied
last
decade.
However,
we
currently
lack
knowledge
core
microbiome
during
ontogenesis
how
it
benefits
growth.We
analyzed
microbial
community
28
samples
from
laboratory
breeding
system,
designed
replicate
aquaculture
environments,
across
six
developmental
stages
(fertilized
eggs
juvenile
stage)
over
three
years-period
examine
microbiomes'
dynamics
stability.
shifts
occurred
larval
every
case.
Application
sophisticated
extraction
methodology,
hybrid
approach
with
abundance-occupancy
analyses
(top
75%
total
reads
>
70%
occupation)
index
calculation,
first
revealed
consisted
Alteromonadaceae
Rhodobacteraceae,
as
well
stage
consisting
pioneer
microbe
Pseudoalteromonadaceae
A.
japonicus,
suggesting
stepwise
establishment
related
feeding
behavior
japonicus.
More
interestingly,
four
ASVs
affiliated
Rhodobacteraceae
were
extracted
microbiome.
One
ASV
(ASV0007)
was
Sulfitobactor
strain
BL28
(Rhodobacteraceae),
isolated
blastula
larvae
2019
raring
batch.
Unexpectedly,
bioassay
retains
growth-promoting
ability.
Further
meta-pangenomics
genome
abundant
metagenomic
sequence
pool,
particular,
post-gut
japonicus.Repeated
rearing
efforts
using
replicating
environments
particular
growth
promoting
ability
microbes,
identified
ASV0007.
Genome
which
makes
us
consider
effective
probiotic
uses
those
for
resource
production
conservation.
study
also
emphasizes
importance
influencing
invertebrates.
Understanding
these
could
offer
pathways
improve
growth,
immunity,
disease
resistance
Nature Communications,
Journal Year:
2025,
Volume and Issue:
16(1)
Published: Jan. 22, 2025
Current
estimates
of
wetland
contributions
to
the
global
methane
budget
carry
high
uncertainty,
particularly
in
accurately
predicting
emissions
from
methane-emitting
wetlands.
Microorganisms
drive
cycling,
but
little
is
known
about
their
conservation
across
To
address
this,
we
integrate
16S
rRNA
amplicon
datasets,
metagenomes,
metatranscriptomes,
and
annual
flux
data
9
wetlands,
creating
Multi-Omics
for
Understanding
Climate
Change
(MUCC)
v2.0.0
database.
This
resource
used
link
microbiome
composition
function
emissions,
focusing
on
methane-cycling
microbes
networks
driving
carbon
decomposition.
We
identify
eight
genera
shared
wetlands
show
wetland-specific
metabolic
interactions
marshes,
revealing
low
connections
between
methanogens
methanotrophs
high-emitting
Methanoregula
emerged
as
a
hub
methanogen
strong
predictor
flux.
In
these
it
also
displays
functional
potential
methylotrophic
methanogenesis,
highlighting
importance
this
pathway
ecosystems.
Collectively,
our
findings
illuminate
trends
microbial
decomposition
while
providing
an
extensive
publicly
available
database
advance
future
research.
The
authors
created
multisite
CH4
fluxes
diverse
differences
cross-feeding
dynamics
that
improve
predictions
Frontiers in Amphibian and Reptile Science,
Journal Year:
2025,
Volume and Issue:
2
Published: Jan. 28, 2025
A
diverse
metabolome
exists
on
amphibian
skin
that
mediates
interactions
between
hosts
and
microbiomes.
Tetrodotoxin
is
one
such
metabolite
occurs
across
a
variety
of
taxa,
particularly
well
studied
in
newts
the
genus
Taricha
are
susceptible
to
infection
with
chytrid
fungi.
The
interaction
tetrodotoxin
microbiome,
including
pathogenic
fungi,
not
understood,
here
we
describe
these
patterns
12
populations
granulosa
T.
torosa
Washington,
Oregon,
California.
We
found
no
correlation
TTX
Batrachochytrium
dendrobatidis
(Bd)
either
or
,
pattern
inconsistent
previous
study.
In
addition,
TTX,
but
Bd,
was
significantly
correlated
microbiome
composition
.
however,
structure.
relationship
differed
species,
significant
correlations
observed
only
which
exhibited
higher
concentrations.
also
detected
abundances
bacterial
taxa
(e.g.,
Pseudomonadaceae)
associated
production
TTX.
These
(ASVs
matching
Aeromonas,
Pseudomonas,
Shewanella
Sphingopyxis
)
were
all
body
sites
previously
sampled
soil
samples.
Our
results
suggest
toxins
can
shape
newt
may
influence
pathogen
through
indirect
mechanisms,
as
showed
direct
inhibition
Bd
B.
salamandrivorans
growth.
Communications Biology,
Journal Year:
2025,
Volume and Issue:
8(1)
Published: March 11, 2025
Abstract
Water
kefir
(WK)
is
an
artisanal
fermented
beverage
made
from
sugary
water,
optional
fruits
and
WK
grains.
grains
can
be
reused
to
start
new
fermentations.
Here
we
investigate
the
microbial
composition
function
of
69
their
ferments
by
shotgun
metagenomics.
A
subset
samples
was
subjected
metabolomic,
including
volatilomic,
analysis.
The
impact
different
fermentation
practices
on
characteristics
analysed
it
noted
that,
for
example,
common
practice
drying
water
significantly
reduces
diversity
negatively
impacts
subsequent
grain
growth.
Metagenomic
analysis
allowed
detection
96
species
within
WK,
definition
core
genera
community
states
after
48
h
fermentation.
total
485
bacterial
metagenome
assembled
genomes
were
obtained
18
putatively
novel
predicted.
Metabolite
volatile
show
associations
between
key
with
flavour
compounds.
We
complex
links
practices,
microbes
product.
results
used
as
a
foundation
selection
large
scale
production
desired
profiles
guide
regulatory
framework
commercial
production.
Marine Mammal Science,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 9, 2025
ABSTRACT
The
spotted
seal
(
Phoca
largha
),
which
primarily
inhabits
the
cold
waters
of
Northwest
Pacific
Ocean,
including
China's
northern
Bohai
and
Yellow
Seas,
exhibits
age‐related
variations
in
its
fecal
microbiome
that
remain
poorly
understood.
This
study
investigated
composition
predicted
functional
capabilities
between
adult
juvenile
seals.
Firmicutes
was
dominant
phylum
both
groups,
with
significant
differences
abundances
Bacteroidota
p
<
0.05).
Furthermore,
higher
taxonomic
diversity
found
adults,
six
genera
compared
to
three
juveniles.
Clostridium
sensu
stricto
1
UCG‐005
were
identified
as
core
genera,
whereas
Bacteroides
Peptoclostridium
specific
adults
Eubacterium
fissicatena
group
Blautia
Moreover,
bacterial
prediction
revealed
a
enrichment
0.01)
Transporters
ABC
transporters
pathways
juveniles,
while
Ribosome
pathway
abundant
0.01).
adults’
displayed
more
complex
stable
interactions
than
Additionally,
phylogenetic
analysis
perfringens
distributed
across
marine
terrestrial
mammals,
Faecalicatena
juveniles
demonstrated
high
host
specificity.
These
findings
help
explain
how
age
diet
shape
gut
mammals.
International Endodontic Journal,
Journal Year:
2025,
Volume and Issue:
unknown
Published: March 14, 2025
Abstract
Aim
To
characterize
the
deep
dentinal
caries
microbiota
in
teeth
diagnosed
with
normal
pulp
(NP),
reversible
pulpitis
(RP),
symptomatic
irreversible
(SIP),
and
asymptomatic
(AIP),
to
identify
potential
key
pathogens
associated
progression,
exploring
their
roles
disease
advancement.
Methodology
In
this
cross‐sectional
study,
we
collected
108
samples,
categorized
into
NP
(
n
=
27),
RP
SIP
AIP
according
American
Association
of
Endodontists'
diagnostic
criteria.
2
samples
were
excluded
due
contamination.
Samples
processed
using
Illumina
MiSeq
high‐throughput
sequencing.
Alpha
beta
diversity,
taxa
abundance
differences,
co‐occurrence
network
analysis,
functional
prediction
evaluated.
Correlation
analysis
between
bacteria
clinical
diagnosis,
signs,
exposure
status
was
performed
Spearman
Mantel
test.
Results
The
bacteriome
exhibited
statistically
significant
differences
among
NP,
RP,
SIP,
groups.
showed
similar
microbial
community
structures,
comparable
alpha
bacterial
phenotypes,
functions,
structures.
contrast,
displayed
distinct
profiles.
characterized
by
higher
diversity
a
greater
gram‐negative
bacteria,
Propionibacterium
Prevotella_7
identified
as
pathogenesis.
On
other
hand,
lower
facultative
anaerobes,
Lactobacillus
Limosilactobacillus
Fusobacterium
,
Prevotella
Treponema
Selenomonas
both
SIP.
Compared
networks
are
more
complex
contain
endodontic
pathogens.
These
form
positive
correlations
each
numerous
negative
lactic
acid
bacteria.
Conclusions
differs
significantly
across
AIP,
exhibit
communities,
whereas
display