Field expedient stool collection methods for gut microbiome analysis in deployed military environments DOI Creative Commons
Car Reen Kok, James B. Thissen,

Michele Cerroni

et al.

mSphere, Journal Year: 2025, Volume and Issue: unknown

Published: May 15, 2025

ABSTRACT Field expedient devices and protocols for the collection, storage, shipment of stool samples in deployed settings are needed advancement microbiome research military health. Relevant assessments include evaluation signatures associated with susceptibility to travelers’ diarrhea recovery gut function following infection. However, inherent biases microbial measurements due preservatives sampling methods unclear should be assessed an accurate microbiome. We performed shotgun metagenomic sequencing compared composition paired fecal collected using Flinters Technology Associates (FTA) cards OMNIgene (OG) Gut tubes, prior during international travel, from 49 adult participants, 39 whom remained asymptomatic 10 experienced diarrhea. Higher concentrations nucleic acid libraries were observed OG samples. A majority genera (82.9%) detected both methods, detections limited one collection method not highly prevalent across present extremely low relative abundances (<0.01%). Differences beta diversity largely explained by inter-individuality composition, followed effect timepoint-disease states. Differential abundance analysis indicated that Corynebacterium Blautia consistently higher all groups FTA respectively. The differences between suggest need consistent standardized within a study. Overall, data presented here could help guide future design study field deployment settings. IMPORTANCE assessment field-deployable sample storage is required reliably capture remote austere locations. This describes comparative metagenomics two different commercially available military-deployed setting. results foundational operational context.

Language: Английский

Field expedient stool collection methods for gut microbiome analysis in deployed military environments DOI Creative Commons
Car Reen Kok, James B. Thissen,

Michele Cerroni

et al.

mSphere, Journal Year: 2025, Volume and Issue: unknown

Published: May 15, 2025

ABSTRACT Field expedient devices and protocols for the collection, storage, shipment of stool samples in deployed settings are needed advancement microbiome research military health. Relevant assessments include evaluation signatures associated with susceptibility to travelers’ diarrhea recovery gut function following infection. However, inherent biases microbial measurements due preservatives sampling methods unclear should be assessed an accurate microbiome. We performed shotgun metagenomic sequencing compared composition paired fecal collected using Flinters Technology Associates (FTA) cards OMNIgene (OG) Gut tubes, prior during international travel, from 49 adult participants, 39 whom remained asymptomatic 10 experienced diarrhea. Higher concentrations nucleic acid libraries were observed OG samples. A majority genera (82.9%) detected both methods, detections limited one collection method not highly prevalent across present extremely low relative abundances (<0.01%). Differences beta diversity largely explained by inter-individuality composition, followed effect timepoint-disease states. Differential abundance analysis indicated that Corynebacterium Blautia consistently higher all groups FTA respectively. The differences between suggest need consistent standardized within a study. Overall, data presented here could help guide future design study field deployment settings. IMPORTANCE assessment field-deployable sample storage is required reliably capture remote austere locations. This describes comparative metagenomics two different commercially available military-deployed setting. results foundational operational context.

Language: Английский

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