Transgene Mapping in Animals: What to Choose? DOI Open Access
A. V. Smirnov, Maksim Makarenko,

A. M. Yunusova

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(10), P. 4705 - 4705

Published: May 14, 2025

The phenomenal progress in biotechnology and genomics is both inspiring overwhelming-a classic curse of choice, particularly when it comes to selecting methods for mapping transgene DNA integration sites. Transgene localization remains a crucial task the validation transgenic mouse or other animal models generated by pronuclear microinjection. Due inherently random nature integration, reliable characterization insertion site essential. Over years, vast number have been developed, new approaches continue emerge, making choice most suitable technique increasingly complex. Factors such as cost, required reagents, data require careful consideration. In this review, we provide structured overview current techniques, which broadly classified into three categories: PCR-based (such inverse PCR TAIL-PCR), next-generation sequencing with target enrichment, long-read platforms (PacBio Oxford Nanopore). To aid decision-making, include comparative table summarizing approximate costs methods. While each approach has its own advantages limitations, highlight our top four recommended methods, believe offer best balance cost-effectiveness, reliability, simplicity identifying

Language: Английский

Transgene Mapping in Animals: What to Choose? DOI Open Access
A. V. Smirnov, Maksim Makarenko,

A. M. Yunusova

et al.

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(10), P. 4705 - 4705

Published: May 14, 2025

The phenomenal progress in biotechnology and genomics is both inspiring overwhelming-a classic curse of choice, particularly when it comes to selecting methods for mapping transgene DNA integration sites. Transgene localization remains a crucial task the validation transgenic mouse or other animal models generated by pronuclear microinjection. Due inherently random nature integration, reliable characterization insertion site essential. Over years, vast number have been developed, new approaches continue emerge, making choice most suitable technique increasingly complex. Factors such as cost, required reagents, data require careful consideration. In this review, we provide structured overview current techniques, which broadly classified into three categories: PCR-based (such inverse PCR TAIL-PCR), next-generation sequencing with target enrichment, long-read platforms (PacBio Oxford Nanopore). To aid decision-making, include comparative table summarizing approximate costs methods. While each approach has its own advantages limitations, highlight our top four recommended methods, believe offer best balance cost-effectiveness, reliability, simplicity identifying

Language: Английский

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