Comparative Genome Sequencing Analysis of Some Novel Feline Infectious Peritonitis Viruses Isolated from Some Feral Cats in Long Island

Viruses, Journal Year: 2025, Volume and Issue: 17(2), P. 209 - 209

Published: Jan. 31, 2025

Feline infectious peritonitis virus (FIPV) remains as one of the leading causes morbidity and mortality in young cats from shelters catteries worldwide. Since little is known about molecular characteristics currently circulating FIPV strains Long Island, New York, samples two shelter submitted to Pathology Diagnostic Services Island University College Veterinary Medicine, with gross microscopic lesions consistent those FIP were processed for isolation, characterization full-length genome decoding. The younger cat, a 1-year-old male (A15) was found dead without previous signs illness. Postmortem examination revealed characterized by vasculitis, necrosis, hemorrhage, pyogranulomatous inflammation confined colon associated lymph nodes. second 7-year-old spayed female (A37) had an identical clinical history similar but widespread lesions, including fibrinous peritoneal effusion, cecal, colonic, renal, hepatic involvement. diagnosis these confirmed immunohistochemistry (IHC) demonstration feline coronavirus antigen using mouse anti-FIPV3-70 monoclonal antibody. Virus isolation saved frozen kidney tissue performed through several subsequent blind passages MDCK Vero cell lines. Confirmation done qRT-PCR, IFA, western blot N protein antibodies, NGS sequencing. sequences isolate decoded next-generation sequencing (NGS) deposited GenBank accession numbers PQ192636 PQ202302. size isolates (29355 29321) nucleotides (nt) length, respectively. While their organization other genomes follows (5'UTR-ORF1ab-S-3abc-M-E-7b-3'UTR-3'), marked differential mutations observed ORF1a/b, S, 3Abc, 7b genes isolates. One notable deletion 34 absent other. We potential recombination events during evolution field parent FECoV-US isolated 1970 minor Canine coronavirus. Our results provide comprehensive analysis novel causing fatal disease Island. surveillance within animal may help early detect unique emerging pathological manifestations develop more targeted prophylactic therapeutic approaches control it.

Language: Английский

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An outbreak of canine coronavirus type 2 in captive snow leopards (Panthera uncia) demonstrates a possible role for felids as mixing vessels for alphacoronaviruses

IJID One Health, Journal Year: 2025, Volume and Issue: unknown, P. 100057 - 100057

Published: March 1, 2025

Language: Английский

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Rapid Clinical Resolution and Differential Diagnosis of a Neurological Case of Feline Infectious Peritonitis (FIP) Using GS-441524

Pathogens, Journal Year: 2025, Volume and Issue: 14(5), P. 424 - 424

Published: April 27, 2025

Case summary: A 2-year-old male neutered domestic shorthair cat was presented with a progressive history of tetraparesis, ataxia, and inappetence over 4 days. physical exam revealed mucopurulent nasal discharge stertor. neurologic multifocal neurolocalization. The non-ambulatory tetraparetic developed seizures while in hospital. Hematologic assessment anemia, hypoalbuminemia hyperglobulinemia. Magnetic resonance imaging (MRI) the brain meningeal contrast enhancement brainstem cervical spine, as well mandibular retropharyngeal lymphadenopathy. Cerebrospinal fluid marked neutrophilic pleocytosis; no infectious organisms were seen. Toxoplasma IgG/IgM Cryptococcus antigen latex agglutination negative. Mandibular abdominal lymph nodes aspirated, cytology mixed inflammation. suspected to have feline peritonitis, aid clinical diagnosis he enrolled research study—with targeted Nanopore-based sequencing specifically identifying characterizing FCoV-1 RNA spinal anal swab, but not urine. treated anticonvulsants (phenobarbital levetiracetam), an antibiotic (ampicillin/clavulanic acid), GS-441524. Neurologic signs did improve on alone improved significantly after two subcutaneous injections received 84-day course GS-441524 and, at time manuscript preparation (over 12 months diagnosis), remains ambulatory seizure-free without recurrence detectable viral shedding feces.

Language: Английский

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Precision and efficacy of RNA-guided DNA integration in high-expressing muscle loci

Molecular Therapy — Nucleic Acids, Journal Year: 2024, Volume and Issue: 35(4), P. 102320 - 102320

Published: Sept. 2, 2024

Gene replacement therapies primarily rely on adeno-associated virus (AAV) vectors for transgene expression. However, episomal expression can decline over time due to vector loss or epigenetic silencing. CRISPR-based integration methods offer promise long-term insertion. While the development of has made substantial progress, identifying optimal insertion loci remains challenging. Skeletal muscle is a promising tissue gene owing low invasiveness intramuscular injections, relative proportion body mass, multinucleated nature muscle, and potential reduced adverse effects. Leveraging endogenous promoters in skeletal we evaluated two highly expressing using homology-independent targeted (HITI) integrate reporter therapeutic genes mouse myoblasts tissue. We hijacked creatine kinase (Ckm) myoglobin (Mb) by co-delivering CRISPR-Cas9 donor plasmid with promoterless constructs encoding green fluorescent protein (GFP) human Factor IX (hFIX). Additionally, deeply profiled our genome transcriptome outcomes from safety proposed sites. This study introduces proof-of-concept technology achieving high-level applications integration-based medicine synthetic biology.Graphical abstract

Language: Английский

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