Antibacterial spectrum of diaphorin, a polyketide produced by a bacterial symbiont of the Asian citrus psyllid

Journal of Invertebrate Pathology, Journal Year: 2025, Volume and Issue: 211, P. 108309 - 108309

Published: March 12, 2025

Diaphorin is a polyketide synthesized by "Candidatus Profftella armatura" (Betaproteobacteria: Burkholderiales), an obligate symbiont of devastating agricultural pest, the Asian citrus psyllid Diaphorina citri (Hemiptera: Psyllidae). Previous studies showed that physiological concentrations diaphorin, which present in D. at 2-20 mM, are inhibitory to various eukaryotes and Bacillus subtilis (Firmicutes: Bacilli) but promote growth metabolic activity Escherichia coli (Gammaproteobacteria: Enterobacterales). However, bacteria examined for diaphorin were limited these two model species, little was known about spectrum essential understanding its effects on microbiota. As first step address this issue, study investigated six bacterial species: Arsenophonus nasoniae, Photorhabdus luminescens, Serratia entomophila, symbiotica (all Gammaproteobacteria: Enterobacterales), Micrococcus luteus Kocuria rhizophila (both Actinobacteria: Micrococcales). The results revealed five milimolar promotes M. inhibits other showing complex not simply determined taxonomic group or cell envelope composition target bacteria. To further assess whether differences susceptibility affect suitability as potential biopesticide, we analyzed mortality after treatment with This only S. entomophila significantly increases mortality, implying when enough bacteria, innate speed immune system have more significant impact controlling citri.

Language: Английский

Microbiome of psyllids of the family Aphalaridae, including Aphalara itadori, a biocontrol agent against Reynoutria spp.

Entomologia Experimentalis et Applicata, Journal Year: 2024, Volume and Issue: unknown

Published: July 19, 2024

Abstract Several European and North American countries have started releasing the Japanese knotweed psyllid, Aphalara itadori (Shinji) (Hemiptera: Aphalaridae), to control knotweed, Reynoutria japonica Houtt. (Polygonaceae), its relatives, which are among worst invasive exotic plants. However, establishing populations of currently released strains in field has not been successful, desiring newly collected lineages. Moreover, little is known about microbiome current strains, potentially impacts properties as biocontrol agents. Hence, this study analyzed microbiota an A. strain on Honshu Island, Japan, along with related species family Aphalaridae, using amplicon sequencing 16S rRNA genes. The localization symbionts identified was further fluorescence situ hybridization. results demonstrated that bacteriome, a specialized organ for microbial symbiosis, maintains dual symbiotic system primary symbiont “ Candidatus Carsonella ruddii” (Gammaproteobacteria: Oceanospirillales: Halomonadaceae) secondary Sodalis sp. Enterobacterales: Pectobacteriaceae), suggesting they evolutionarily stable obligate mutualists . central area bacteriome containing comprised uninucleate bacteriocytes nuclei larger than those harboring This observation contrasted previous reports various psyllid lineages housed syncytium smaller No plant pathogens or parasitic manipulators insect reproduction were strain, indicating suitability agent, posing minimum risk ecosystem. Besides distinct lineages, independently acquired by Craspedolepta miyatakeai Klimaszewski ambiguous Enterobacterales Epheloscyta kalopanacis Loginova identified. Only found Togepsylla matsumurana Kuwayama. These indicate repeated infections replacements bacterial during evolution Psylloidea, providing deeper insights into microbe‐psyllid interactions.

Language: Английский

Highly Reduced Complementary Genomes of Dual Bacterial Symbionts in the Mulberry Psyllid <i>Anomoneura mori</i>

Microbes and Environments, Journal Year: 2024, Volume and Issue: 39(3), P. n/a - n/a

Published: Jan. 1, 2024

The genomes of obligately host-restricted bacteria suffer from accumulating mildly deleterious mutations, resulting in marked size reductions. Psyllids (Hemiptera) are phloem sap-sucking insects with a specialized organ called the bacteriome, which typically harbors two vertically transmitted bacterial symbionts: primary symbiont "Candidatus Carsonella ruddii" (Gammaproteobacteria) and secondary that is phylogenetically diverse among psyllid lineages. several lineages were revealed to be markedly reduced (158-174‍ ‍kb), AT-rich (14.0-17.9% GC), structurally conserved similar gene inventories devoted synthesizing essential amino acids scarce sap. However, limited genomic information currently available on symbionts. Therefore, present study investigated bacteriome-associated dual symbionts, Secondary_AM Carsonella_AM, mulberry Anomoneura mori (Psyllidae). results obtained genome as small (229,822 bp, 17.3% GC) those lineages, including Carsonella_AM (169,120 16.2% implying an evolutionarily ancient obligate mutualist, Carsonella. Phylogenomic ana-lyses showed sister Psyllophila symbiotica" Cacopsylla spp. (Psyllidae), recently reported (221-237‍ ‍kb, 17.3-18.6% GC). highly synteny, sharing all genes for complementing incomplete tryptophan biosynthetic pathway B vitamins. sulfur assimilation carotenoid-synthesizing only retained Psyllophila, respectively, indicating ongoing silencing. Average nucleotide identity, ortholog similarity, genome-wide substitution rates suggest Secondary_AM/Psyllophila more labile than genomes.

Language: Английский