Cited by Environmental surface monitoring as a noninvasive method for SARS-CoV-2 surveillance in community settings: Lessons from a university campus study

The emerging challenge of FLiRT variants: KP.1.1 and KP.2 in the global pandemic landscape DOI

Prithiv K R Kumar,

Jezina Jayan,

Rakesh Sharma

et al.

QJM, Journal Year: 2024, Volume and Issue: 117(7), P. 485 - 487

Published: June 12, 2024

The SARS-CoV-2 virus has undergone substantial evolution, leading to emergence of new FLiRT variants characterized by specific spike mutations-F L at position 456 and R T 346-enhancing their transmissibility immune evasion capabilities. Particularly, KP.2 shows a significant increase in cases the USA, indicating potential shift pandemic landscape due its greater ability evade vaccine-induced immunity higher effective reproduction number compared JN.1. This evolving scenario underscores need for continuous monitoring adaptive response strategies address challenges posed these variants. abstract examines KP1.1, descendants Omicron JN.1 variant, as they draw global attention amidst ongoing coronavirus disease-2019 pandemic.

Language: Английский

Citations

Wastewater surveillance for viral pathogens: A tool for public health DOI

Matheus Carmo dos Santos,

Ana Clara Cerqueira Silva,

Carine dos Reis Teixeira

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(13), P. e33873 - e33873

Published: June 29, 2024

A focus on water quality has intensified globally, considering its critical role in sustaining life and ecosystems. Wastewater, reflecting societal development, profoundly impacts public health. Wastewater-based epidemiology (WBE) emerged as a surveillance tool for detecting outbreaks early, monitoring infectious disease trends, providing real-time insights, particularly vulnerable communities. WBE aids tracking pathogens, including viruses, sewage, offering comprehensive understanding of community health lifestyle habits. With the rise global COVID-19 cases, gained prominence, aiding SARS-CoV-2 levels worldwide. Despite advancements treatment, poorly treated wastewater discharge remains threat, amplifying spread water-, sanitation-, hygiene (WaSH)-related diseases. WBE, serving complementary surveillance, is pivotal community-level viral infections. However, there untapped potential to expand surveillance. This review emphasizes importance link between health, highlighting need further integration into management.

Language: Английский

Citations

Genomic surveillance reveals early detection and transition of delta to omicron lineages of SARS-CoV-2 variants in wastewater treatment plants of Pune, India DOI

Vinay Rajput, Rinka Pramanik,

Vinita Malik

et al.

Environmental Science and Pollution Research, Journal Year: 2023, Volume and Issue: 30(56), P. 118976 - 118988

Published: Nov. 3, 2023

Language: Английский

Citations

Hyperplex PCR enables highly multiplexed analysis of point mutations in wastewater: long-term SARS-CoV-2 variant surveillance in Sweden as a case study DOI

Ruben R. G. Soares,

Javier Edo Varg,

Attila Szabó

et al.

Water Research, Journal Year: 2025, Volume and Issue: 274, P. 123154 - 123154

Published: Jan. 16, 2025

Language: Английский

Citations

Wastewater-based SARS-CoV-2 surveillance and sequencing DOI

Alice Michie

Microbiology Australia, Journal Year: 2024, Volume and Issue: 45(1), P. 8 - 12

Published: March 13, 2024

Though most often associated with poliovirus surveillance, wastewater-based surveillance (WBS) can be employed for viruses shed in human excreta (faeces, urine, skin, sputum, blood) that may enter the wastewater system, including SARS-CoV-2. WBS has been widely adopted during COVID-19 pandemic, to complement clinical monitoring community burden and implementing timely public health interventions. As is a non-biased, composite sample, it provide population-level information near real-time, cost-effective manner compared similarly scaled surveillance. In many instances, data gathered from wastewater, viral loads (quantified by RT-qPCR) variant detections (determined through partial or whole genome sequencing), have predictive of what observed eventually cases. Newly emergent lineages, recent BA.2.86 variant, detected samples prior their detection specimens. There remain challenges genomic analysis presence RT-qPCR inhibitors, degraded nucleic acid lack consistent standardised methodology between reporting labs. The wide adoption practices provides an excellent opportunity expand this method other pathogens importance. Herein, broad overview field will provided discussion on its advantages applications, challenges, how being utilised characterise circulating SARS-CoV-2 lineages sequencing.

Language: Английский

Citations

RBD amplicon sequencing of wastewater reveals patterns of variant emergence and evolution DOI

Xingwen Chen,

John Balliew,

Cici Bauer

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 12, 2024

Abstract Rapid evolution of SARS-CoV-2 has resulted in the emergence numerous variants, posing significant challenges to public health surveillance. Clinical genome sequencing, while valuable, limitations capturing full epidemiological dynamics circulating variants general population. This study utilized receptor-binding domain (RBD) amplicon sequencing wastewater samples monitor community and El Paso, TX. Over 17 months, we identified 91 observed waves dominant transitioning from BA.2 BA.2.12.1, BA.4&5, BQ.1, XBB.1.5. Our findings demonstrated early detection identification unreported outbreaks, showing strong consistency with clinical data at local, state, national levels. Alpha diversity analyses revealed periodical variations, highest winter outbreak lag phases, likely due lower competition among before growth phase. The underscores importance low transmission periods for rapid mutation variant evolution. highlights effectiveness integrating RBD surveillance tracking viral evolution, understanding emergence, enhancing preparedness.

Language: Английский

Citations

Tracking the Time Lag between SARS-CoV-2 Wastewater Concentrations and Three COVID-19 Clinical Metrics: A 21-Month Case Study in the Tricounty Detroit Area, Michigan DOI

Liang Zhao, Russell A. Faust, Randy E. David

et al.

Journal of Environmental Engineering, Journal Year: 2023, Volume and Issue: 150(1)

Published: Oct. 20, 2023

Wastewater surveillance has been widely implemented to monitor COVID-19 incidences in communities worldwide. One notable application of wastewater is for providing early warnings disease outbreaks. Many studies have reported time lags between severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) concentrations and confirmed clinical cases. To our best knowledge, only a few date explored SARS-CoV-2 other metrics. In this study, we investigated three metrics: cases, hospitalizations, intensive care unit (ICU) admissions, the Tricounty Detroit Area, Michigan, US. The metrics were dated September 1, 2020, October 31, 2022, collected from public data sources. N1 N2 gene May generated using two sampling concentration methods: virus adsorption-elution (VIRADEL) polyethylene glycol precipitation (PEG). recently published study. Time-lagged cross correlation was estimate Original normalized by flow parameters through nine approaches impact on lags. Vector autoregression models established analyze relationship results indicate that VIRADEL preceded all prior Omicron surge, instance, 32, 47, 51 days preceding ICU respectively (gene unit: gc/day). When translated health context, these become critical lead times officials prepare react. During there significant reductions lags, with measurements trailing total admissions. PEG lagged behind did not provide surges.

Language: Английский

Citations

Wastewater Surveillance of SARS-CoV-2 in Zambia: An Early Warning Tool DOI

Doreen Mainza Shempela, Walter Muleya, Steward Mudenda

et al.

Published: June 26, 2024

Wastewater-based surveillance has emerged as an important method for monitoring SARS-CoV-2. This study investigated the presence of SARS-CoV-2 in wastewater Zambia. We conducted a longitudinal Copperbelt and Eastern provinces Zambia from October 2023 to December during which 155 samples were collected. The subjected three different concentration methods, namely bag-mediated filtration, skimmed milk flocculation, polythene glycol-based assays. Molecular detection nucleic acid was using real-time PCR. Whole genome sequencing Illumina COVIDSEQ assay. Of samples, 62 (40%) tested positive these, 13 sequences sufficient length determine lineages obtained two phylogenetically analyzed. Various Omicron subvariants detected including BA.5, XBB.1.45, BA.2.86, JN.1. Some these have been clinical cases Interestingly, phylogenetic analysis positioned sequence Province B.1.1.529 clade, suggesting that earlier variants late 2021 could still be circulating may not wholly replaced by newer subvariants. stresses need integrating into mainstream strategies circulation

Language: Английский

Citations

Co-occurrence of SARS-CoV-2 variants in rivers and sewage in India and Brazil DOI

Cristiane Thompson,

Camille V. Leal, Renan Faustino

et al.

The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 958, P. 178089 - 178089

Published: Dec. 20, 2024

Language: Английский

Citations

Revealing patterns of SARS-CoV-2 variant emergence and evolution using RBD amplicon sequencing of wastewater DOI

Xingwen Chen,

John Balliew,

Cici Bauer

et al.

Journal of Infection, Journal Year: 2024, Volume and Issue: 89(5), P. 106284 - 106284

Published: Sept. 26, 2024

Rapid evolution of SARS-CoV-2 has resulted in the emergence numerous variants, posing significant challenges to public health surveillance. Clinical genome sequencing, while valuable, limitations capturing full epidemiological dynamics circulating variants general population. This study aimed monitor variant community and using receptor-binding domain (RBD) amplicon sequencing wastewater samples.

Language: Английский

Citations