Trends in Parasitology, Journal Year: 2023, Volume and Issue: 40(2), P. 93 - 95
Published: Dec. 29, 2023
Language: Английский
Applied Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 109(1)
Published: Jan. 29, 2025
Abstract The rapid advancement of nanotechnology, particularly in the realm pharmaceutical sciences, has significantly transformed potential for treating life-threatening diseases. A pivotal aspect this evolution is emergence “green nanotechnology,” which emphasizes environmentally sustainable synthesis raw materials through biological processes. This review focuses on and application zinc oxide (ZnO) nanoparticles (NPs) from probiotic bacteria, those sourced wastewater. Microorganisms wastewater tolerate harmful elements enzymatically convert toxic heavy metals into eco-friendly materials. These bacteria are instrumental ZnO NPs exhibit remarkable antimicrobial properties with diverse industrial applications. As challenge drug-resistant pathogens escalates, innovative strategies combating microbial infections essential. explores intersection microbiology, antibacterial resistance, highlighting importance selecting suitable synthesizing potent activity. Additionally, addresses biofunctionalization their applications environmental remediation therapeutic innovations, including wound healing, antibacterial, anticancer treatments. Eco-friendly NP relies identification these “nano-factories.” Targeting can uncover new capabilities, advancing friendly production methods. Key points • Innovative needed to combat like MRSA. Wastewater-derived an method synthesis. show significant activity against various pathogens.
Language: Английский
Citations
1
Human Genomics, Journal Year: 2024, Volume and Issue: 18(1)
Published: Feb. 6, 2024
Abstract Background Periodic bioinformatics-based screening of wastewater for assessing the diversity potential human viral pathogens circulating in a given community may help to identify novel or potentially emerging infectious diseases. Any identified contigs related viruses should be confirmed with targeted and clinical testing. Results During COVID-19 pandemic, untreated samples were collected 1-year period from Great Lakes Water Authority Wastewater Treatment Facility Detroit, MI, USA, population both centralized interceptor sites localized neighborhood sewersheds was investigated. Clinical cases diseases caused by tabulated compared data monitoring. In addition Betacoronavirus , comparison using assembled against custom Swiss-Prot virus database indicated prevalence other pathogenic genera, including: Orthopoxvirus Rhadinovirus, Parapoxvirus Varicellovirus, Hepatovirus, Simplexvirus, Bocaparvovirus, Molluscipoxvirus, Parechovirus, Roseolovirus, Lymphocryptovirus, Alphavirus, Spumavirus, Lentivirus, Deltaretrovirus, Enterovirus, Kobuvirus, Gammaretrovirus, Cardiovirus, Erythroparvovirus, Salivirus, Rubivirus, Orthohepevirus, Cytomegalovirus, Norovirus, Mamastrovirus . Four nearly complete genomes recovered Astrovirus, Norovirus Betapolyomavirus genera species identified. Conclusions The presented findings are primarily at genus level can serve as preliminary “screening” tool that indication initiate further testing confirmation presence associated disease. Integrating innovative environmental microbiology technologies like metagenomic sequencing epidemiology offers significant opportunity improve monitoring of, predictive intelligence for, viruses, wastewater.
Language: Английский
Citations
8
Environmental Sciences Europe, Journal Year: 2024, Volume and Issue: 36(1)
Published: April 23, 2024
Abstract This study developed an integrated wastewater treatment system that combines upflow anaerobic sludge blanket (UASB), downflow hanging non-woven fabric (DHNW), and baffled reactor (ABR) to explore the effect of stages on diversity microeukaryotic communities. aimed bridge knowledge gap regarding influence community diversity. Through 18S rRNA amplicon sequencing, we identified unique communities across different stages, with aerobic phase hosting 35.77% sequence variants (ASVs). The results principal component analysis (PCA) non-multidimensional scale (nMDS) demonstrated significant both environmental factors Ciliophora was notably abundant in effluent (42.09%) (17.11%). stage dominated by Ochrophyta, a diverse group algae instrumental nutrient removal, such as nitrogen phosphorus, through biological processes. A redundancy (RDA) revealed positive correlation between chemical biochemical oxygen demand Cryptomycotina, highlighting its potential bioindicator for efficacy. detection protozoan species, Acanthamoeba castellanii Vermamoeba vermiformis , outlet poses health risks, whereas Cryptosporidium sp. found inlet but not outlet. Our reveals complex nature implications performance public health.
Language: Английский
Citations
6
BMC Research Notes, Journal Year: 2025, Volume and Issue: 18(1)
Published: Jan. 7, 2025
The World Health Organization (WHO) has declared antimicrobial resistance (AMR) as one of the top threats to global public health. While AMR surveillance human clinical isolates is well-established in many countries, increasing threat intensified efforts detect antibiotic genes (ARGs) accurately and sensitively environmental samples, wastewater, animals, food. Using five ARGs 16S rRNA gene, we compared quantitative PCR (qPCR) metagenomic sequencing (MGS), two commonly used methods uncover wastewater resistome. We both by evaluating ARG detection through a municipal treatment chain. Our results demonstrate that qPCR was more sensitive than MGS, particularly diluted samples with low concentrations such oxidation pond water. However, MGS potentially specific less risk off-target binding concentrated raw sewage. analysis revealed multiple subtypes each gene which could not be distinguished qPCR; these varied across different sample types. findings affect conclusions can drawn when comparing types, terms inferring removal rates or origins genes. conclude appear suitable profile resistome other depending on research question type sample.
Language: Английский
Citations
0
Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 487, P. 137254 - 137254
Published: Jan. 16, 2025
Language: Английский
Citations
0
Environmental Science Water Research & Technology, Journal Year: 2025, Volume and Issue: unknown
Published: Jan. 1, 2025
Two photo-sequencing batch reactors (PSBR) fed with real wastewater were evaluated to understand the elimination and particle association of fecal indicator bacteria (FIB) coliphages.
Language: Английский
Citations
0
Bioresource Technology, Journal Year: 2025, Volume and Issue: unknown, P. 132532 - 132532
Published: April 1, 2025
Language: Английский
Citations
0
Food and Waterborne Parasitology, Journal Year: 2025, Volume and Issue: unknown, P. e00265 - e00265
Published: April 1, 2025
Language: Английский
Citations
0
Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)
Published: Sept. 12, 2023
The phylum Apicomplexa comprises a large group of intracellular protozoan parasites. These microorganisms are known to infect variety vertebrate and invertebrate hosts, leading significant medical veterinary conditions such as toxoplasmosis, cryptosporidiosis, theileriosis, eimeriosis. Despite their importance, comprehensive data on diversity distribution, especially in riverine environments, remain scant. To bridge this knowledge gap, we utilized next-generation high-throughput 18S rRNA amplicon sequencing powered by PacBio technology explore the composition taxa. Principal component analysis (PCA) principal coordinate (PCoA) indicated habitat heterogeneity for physicochemical parameters community. results were supported PERMANOVA (P < 0.001), ANOSIM Cluster analysis, Venn diagram. Dominant genera inlet samples included Gregarina (38.54%), Cryptosporidium (32.29%), Leidyana (11.90%). In contrast, outlet dominated Babesia, Cryptosporidium, Theileria. While surface water revealed 16% 8.33% relative abundance Toxoplasma respectively. our knowledge, high throughput covered wide range parasites Egypt first time, which could be useful legislation standards drinking wastewater reuse.
Language: Английский
Citations
6
Environment & Health, Journal Year: 2023, Volume and Issue: 2(2), P. 68 - 75
Published: Dec. 21, 2023
The recycling of biowaste from municipal wastewater treatment plants (WWTPs) in agriculture represents a circular economy-driven source water and nutrients to support food system sustainability. However, may represent the emerging hazards anthropogenic animal origin that can transfer agricultural soils related production, posing risk consumers' health, as case outbreaks due consumption ready-to-eat leafy vegetables contaminated with pathogenic E. coli. From this perspective, we propose combined strategy based on both classical methods culture-independent metagenomics approaches identify microbial relevant foodborne diseases WWTP-related biowastes. virulence genes targeted by real-time PCR, performed before after enrichment raw samples, proxy for viability pathogens, presence which is then confirmed via microbiological methods. Bioinformatics analysis shotgun metagenomic sequences could assess associated resistance specific antimicrobials followed phenotypic confirmation cultivation samples predicted molecules. Bacterial 16S rDNA supports traceability their taxonomic composition. This would "One Health" Action cross-cutting assessment food-borne risks along chain.
Language: Английский
Citations
4