Frontiers in Immunology,
Journal Year:
2024,
Volume and Issue:
15
Published: Nov. 18, 2024
Porcine
deltacoronavirus
(PDCoV),
a
newly
discovered
intestinal
coronavirus,
has
rapidly
spread
among
pigs
worldwide
and
shown
the
potential
for
cross-species
infection.
However,
interaction
mechanism
between
PDCoV
host’s
antiviral
response
is
still
poorly
understood.
In
this
study,
an
E3
ubiquitin
ligase
FBXW8
was
explored
on
proliferation.
Our
findings
demonstrate
that
infection
increases
expression
of
through
p65-mediated
activation
its
promoter.
We
also
suppresses
replication
by
directly
targeting
inducing
degradation
PDCoV-encoded
nucleocapsid
(N)
protein.
Interestingly,
catalyzes
K48-linked
polyubiquitination
N
protein
at
unique
lysine-rich
region
(KR).
Furthermore,
we
observed
FBXW8-ubiquitinated
interacts
with
NDP52,
cargo
receptor,
leading
to
autophagic
instead
proteasomal
degradation.
summary,
these
reveal
as
novel
host
factor
involved
in
It
mediates
NDP52-dependent
These
results
provide
new
insights
target
defenses
against
PDCoV.
AJP Cell Physiology,
Journal Year:
2023,
Volume and Issue:
325(5), P. C1190 - C1200
Published: Sept. 4, 2023
Interstitial
lung
diseases
can
result
in
poor
patient
outcomes,
especially
idiopathic
pulmonary
fibrosis
(IPF),
a
severe
interstitial
disease
with
unknown
causes.
The
lack
of
treatment
options
requires
further
understanding
the
pathological
process/mediators.
Membrane-associated
RING-CH
8
(MARCH8)
has
been
implicated
immune
function
regulation
and
inflammation,
however,
its
role
development
particularly
fibroblast
to
myofibroblast
transition
(FMT)
remains
gap
existing
knowledge.
In
this
study,
we
demonstrated
decreased
MARCH8
expression
patients
IPF
compared
non-PF
controls
bleomycin-induced
PF.
TGF-β
dose-
time-dependently
normal
human
(HLFs),
along
induction
FMT
markers
α-SMA,
collagen
type
I
(Col-1),
fibronectin
(FN).
Interestingly,
overexpression
significantly
suppressed
TGF-β-induced
Col-1,
FN.
By
contrast,
knockdown
using
siRNA
upregulated
basal
α-SMA/Col-1/FN.
Moreover,
enhanced
marker
expression.
These
data
clearly
show
that
is
critical
"brake"
for
potentially
affects
We
found
mRNA
proteasome
inhibitor
MG132
failed
block
decrease
induced
by
TGF-β.
Conversely,
decreases
levels
time-dependent
manner,
suggesting
transcriptional
Mechanistically,
Smad2/3
phosphorylation,
which
may
account
observed
effects.
Taken
together,
study
an
unrecognized
negatively
regulating
profibrogenic
responses
relevant
diseases.
Cells,
Journal Year:
2024,
Volume and Issue:
13(8), P. 698 - 698
Published: April 17, 2024
The
cellular
transmembrane
protein
MARCH8
impedes
the
incorporation
of
various
viral
envelope
glycoproteins,
such
as
HIV-1
glycoprotein
(Env)
and
vesicular
stomatitis
virus
G-glycoprotein
(VSV-G),
into
virions
by
downregulating
them
from
surface
virus-producing
cells.
This
downregulation
significantly
reduces
efficiency
infection.
In
this
study,
we
aimed
to
further
characterize
host
investigating
its
species
specificity
domains
responsible
for
antiviral
activity,
well
ability
inhibit
cell-to-cell
We
found
that
function
is
conserved
in
rhesus
macaque,
mouse,
bovine
versions.
RING-CH
these
versions
are
functionally
important
inhibiting
Env
VSV-G-pseudovirus
infection,
whereas
tyrosine
motifs
crucial
former
only,
consistent
with
findings
human
MARCH8.
Through
analysis
chimeric
proteins
between
non-antiviral
MARCH3,
determined
both
N-terminal
C-terminal
cytoplasmic
tails,
presumably
domain,
critical
activity.
Notably,
unable
block
likely
due
insufficient
Env.
These
offer
insights
understanding
biology
protein.
Autophagy,
Journal Year:
2024,
Volume and Issue:
20(12), P. 2785 - 2803
Published: Aug. 12, 2024
Rabies
virus
causes
an
estimated
59,000
annual
fatalities
worldwide
and
promising
therapeutic
treatments
are
necessary
to
develop.
In
this
study,
affinity
tag-purification
mass
spectrometry
was
employed
delineate
RABV
glycoprotein
host
protein
interactions,
PDIA3/ERP57
identified
as
a
potential
inhibitor
of
infection.
PDIA3
restricted
infection
with
follow
mechanisms:
mediated
the
degradation
G
by
targeting
lysine
332
via
selective
macroautophagy/autophagy
pathway;
The
interactor,
AP3B1
(adaptor
related
complex
3
subunit
beta
1)
indispensable
in
PDIA3-triggered
protein;
Furthermore,
competitively
bound
NCAM1/NCAM
(neural
cell
adhesion
molecule
block
G,
hindering
viral
entry
into
cells.
190-199
aa
residues
were
sufficient
defend
against
RABV.
These
results
demonstrated
biologics
that
target
or
utilize
peptide
treat
clinical
rabies.
Frontiers in Immunology,
Journal Year:
2024,
Volume and Issue:
15
Published: Nov. 18, 2024
Porcine
deltacoronavirus
(PDCoV),
a
newly
discovered
intestinal
coronavirus,
has
rapidly
spread
among
pigs
worldwide
and
shown
the
potential
for
cross-species
infection.
However,
interaction
mechanism
between
PDCoV
host’s
antiviral
response
is
still
poorly
understood.
In
this
study,
an
E3
ubiquitin
ligase
FBXW8
was
explored
on
proliferation.
Our
findings
demonstrate
that
infection
increases
expression
of
through
p65-mediated
activation
its
promoter.
We
also
suppresses
replication
by
directly
targeting
inducing
degradation
PDCoV-encoded
nucleocapsid
(N)
protein.
Interestingly,
catalyzes
K48-linked
polyubiquitination
N
protein
at
unique
lysine-rich
region
(KR).
Furthermore,
we
observed
FBXW8-ubiquitinated
interacts
with
NDP52,
cargo
receptor,
leading
to
autophagic
instead
proteasomal
degradation.
summary,
these
reveal
as
novel
host
factor
involved
in
It
mediates
NDP52-dependent
These
results
provide
new
insights
target
defenses
against
PDCoV.
