Regulators H-NS and LeuO inversely control swarming motility and biofilm formation inVibrio parahaemolyticus DOI Open Access

Soham M. Bhide,

J.G. Tague,

J. A. Andrews

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 9, 2024

Vibrio parahaemolyticus is a halophile present in marine environments worldwide and leading cause of bacterial seafood-borne gastroenteritis. Free living planktonic cells can either attach to surfaces form swarming or develop into sessile three-dimensional biofilm structure. Swarming motility requires lateral flagella ( laf operon) the expression surface sensing operon scrABC produce spreading cauliflower colony morphology. Biofilms are formed from capsule polysaccharide (CPS) encoded by cpsA-K that positively regulated CpsR CpsQ. In enteric bacteria, H-NS global gene silencer LeuO an antagonist H-NS. this work, we examined role these regulators decision between behaviors using deletion mutants leuO, hns, double leuO/hns . The wild type Δ leuO strains produced colonies whereas hns hyper leuO/ phenotype reverted type. Transcriptional reporter assays P lafB-gfp scrA - gfp showed significantly increased fluorescence compared mutant, levels scrA-gfp CPS assays, had less dense rugose morphology smooth colony, which also biofilm. opaque more Reporter cpsA-gfp cpsR-gfp confirmed roles both formation. Importance This study determined controlling two based behaviors, formation V. effects deletions , mutant was negative regulator swarming, but positive mechanism control part due inhibition at loci required for

Language: Английский

Antibacterial and antibiofilm activities of iodinated hydrocarbons against Vibrio parahaemolyticus and Staphylococcus aureus DOI Creative Commons

Oluwatosin Oluwaseun Faleye,

Olajide Sunday Faleye,

Jin-Hyung Lee

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: April 22, 2024

Abstract Food-related illnesses have become a growing public concern due to their considerable socioeconomic and medical impacts. Vibrio parahaemolyticus Staphylococcus aureus been implicated as causative organisms of food-related infections poisoning, both can form biofilms which confer antibiotic resistance. Hence, the need for continuous search compounds with antibiofilm antivirulence properties. In this study, 22 iodinated hydrocarbons were screened activity, these, iodopropynyl butylcarbamate (IPBC) was found effectively control biofilm formation pathogens MIC 50 µg/mL bactericidal V. S. . Microscopic studies confirmed IPBC inhibits bacteria also disrupted mixed formation. Furthermore, suppressed virulence activities such motility hemolytic activity cell surface hydrophobicity It exhibited preservative potential against in shrimp model. membrane differentially affected gene expressions related virulence. Additionally, it displayed broad-spectrum other clinically relevant pathogens. These findings indicate offers means controlling mediated by biofilms.

Language: Английский

Citations

5
GepA, a GGDEF-EAL protein, regulates biofilm formation and swimming motility in Vibrio parahaemolyticus DOI
Miaomiao Zhang, Yurui Zhu, Xue Li

et al.

Archives of Microbiology, Journal Year: 2025, Volume and Issue: 207(5)

Published: March 22, 2025

Language: Английский

Citations

0
Insights on the regulation and function of the CRISPR/Cas transposition system located in the pathogenicity island VpaI-7 from Vibrio parahaemolyticus RIMD2210633 DOI Creative Commons

Jesús E. Alejandre-Sixtos,

Kebia Aguirre-Martínez,

Jessica Cruz-López

et al.

Infection and Immunity, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

CRISPR/Cas-mediated transposition is a recently recognized strategy for horizontal gene transfer in variety of bacterial species. However, our understanding the factors that control their function natural hosts still limited. In this work, we report initial genetic characterization elements associated with CRISPR/Cas-transposition machinery (CASTm) from Vibrio parahaemolyticus (VpaCASTm), which are encoded within pathogenicity island VpaI-7. Our results revealed components VpaCASTm and CRISPR arrays (VpaCAST system) transcriptionally active native context. Furthermore, were able to detect presence polycistrons several internal promoters loci compose VpaCAST system. also suggest activity promoter atypical array not repressed by baseline its known regulator VPA1391 V. parahaemolyticus. addition, found tniQ was modulated regulatory cascade involving ToxR, LeuO, H-NS. Since it previously reported system less efficient than VchCAST at promoting miniaturized CRISPR-associated transposon (mini-CAST) Escherichia coli, analyzed if efficiency mediated could be enhanced inside host We provide evidence might case, suggesting there induction enable more CASTs.IMPORTANCEMobile such as transposons play important roles evolutionary trajectories genomes. The success dissemination depends on ability carry selectable markers improve fitness cell or addictive traits toxin-antitoxin systems. Here aimed characterize carries disseminate multiple virulence factors. This belongs discovered family whose guided crRNA. showed transcribed likely host-associated favor over coli.

Language: Английский

Citations

0
The LuxO-OpaR quorum-sensing cascade differentially controls Vibriophage VP882 lysis-lysogeny decision making in liquid and on surfaces DOI Creative Commons
Francis J. Santoriello, Bonnie L. Bassler

PLoS Genetics, Journal Year: 2024, Volume and Issue: 20(7), P. e1011243 - e1011243

Published: July 30, 2024

Quorum sensing (QS) is a process of cell-to-cell communication that bacteria use to synchronize collective behaviors. QS relies on the production, release, and group-wide detection extracellular signaling molecules called autoinducers. Vibrios two systems: LuxO-OpaR circuit VqmA-VqmR circuit. Both circuits control group behaviors including biofilm formation surface motility. The Vibrio parahaemolyticus temperate phage φVP882 encodes VqmA homolog (called VqmAφ). When VqmAφ produced by lysogens, it binds host-produced autoinducer DPO launches lytic cascade. This activity times induction lysis with high host cell density presumably promotes maximal transmission new cells. Here, we explore whether, in addition from lysogeny, controls initial establishment lysogeny naïve Using mutagenesis, infection assays, phenotypic analyses, show connects its lysis-lysogeny decision both whether resides liquid or surface. Host cells low-cell-density state primarily undergo lysogenic conversion. regulator LuxO~P conversion planktonic By contrast, ScrABC surface-sensing system regulates surface-associated abundance second messenger molecule cyclic diguanylate, which turn, modulates scrABC operon only expressed when repressor, OpaR, absent. Thus, at low density, QS-dependent derepression drives These results demonstrate integrates cues multiple sensory pathways into lifestyle making upon cell.

Language: Английский

Citations

2
Regulators H-NS and LeuO inversely control swarming motility and biofilm formation inVibrio parahaemolyticus DOI Open Access

Soham M. Bhide,

J.G. Tague,

J. A. Andrews

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Jan. 9, 2024

Vibrio parahaemolyticus is a halophile present in marine environments worldwide and leading cause of bacterial seafood-borne gastroenteritis. Free living planktonic cells can either attach to surfaces form swarming or develop into sessile three-dimensional biofilm structure. Swarming motility requires lateral flagella ( laf operon) the expression surface sensing operon scrABC produce spreading cauliflower colony morphology. Biofilms are formed from capsule polysaccharide (CPS) encoded by cpsA-K that positively regulated CpsR CpsQ. In enteric bacteria, H-NS global gene silencer LeuO an antagonist H-NS. this work, we examined role these regulators decision between behaviors using deletion mutants leuO, hns, double leuO/hns . The wild type Δ leuO strains produced colonies whereas hns hyper leuO/ phenotype reverted type. Transcriptional reporter assays P lafB-gfp scrA - gfp showed significantly increased fluorescence compared mutant, levels scrA-gfp CPS assays, had less dense rugose morphology smooth colony, which also biofilm. opaque more Reporter cpsA-gfp cpsR-gfp confirmed roles both formation. Importance This study determined controlling two based behaviors, formation V. effects deletions , mutant was negative regulator swarming, but positive mechanism control part due inhibition at loci required for

Language: Английский

Citations

0