Epidemiological and molecular characteristics of extraintestinal pathogenic escherichia coli isolated from diseased cattle and sheep in Xinjiang, China from 2015 to 2019

BMC Veterinary Research, Journal Year: 2025, Volume and Issue: 21(1)

Published: Jan. 30, 2025

Escherichia coli has become a common causative agent of infections in animals, inflicting serious economic losses on livestock production and posing threat to public health. infection is tends be complex Xinjiang, major region cattle sheep breeding China. This study aims explore the current status molecular characteristics as part disease prevention control strategy. Herein we isolated Extraintestinal pathogenic (ExPEC) from liver, spleen, lung, heart, lymph nodes infected (Xinjiang, China), phylogenetic grouping, serotyping, multilocus sequence typing were performed determine epidemic characteristics. We also assessed their biofilm formation ability. A total 132 strains ExPEC identified diseased sheep, belonging 7 groups. B1 are advantageous Further, 22 serogroups found, with O101 (26/132), O154 (14/132), O65 (8/132) being predominant ones. Among seven types by typing, ST10 was most common, followed ST23 ST457. Of 132, 105 (79.5%) able form biofilms: 15 (11.4%) strong, 28 (21.2%) medium, 62 (47%) weak producers. These findings will contribute better understanding epidemiology China, can applied development, prevention, future diagnostic tools vaccine.

Language: Английский

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Evolution of pathogenic Escherichia coli harboring the transmissible locus of stress tolerance: from food sources to clinical environments

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 11, 2025

Escherichia coli (E. coli) carrying the transmissible locus of stress tolerance (tLST) are able to overcome numerous environmental challenges. In our in-silico study, we aimed characterize tLST in terms its variants 793 genomes E. from Brazil originating food, and clinical (animal human) sources, perform a temporal analysis order identify historical moment emergence. We also analyzed presence two Yersinia high pathogenicity island (HPI) genomes, describing other genes accessory for resistance, persistence, mobile elements (plasmids) sequence types. The prevalence was 10% Brazil, predominantly observed milk-originating within prevalent tLSTCP010237 variant. sources (clinical/environmental), only part present. Remarkably, pinpointed emergence back around 1914, coinciding with major societal events. Regarding virulence genes, found 38.5% HPI Y. pestis across all sources. Our global showed diversity milk (+ 100 genes). These stood out overall metadata presenting greater variety resistance stresses, such as metals, biocides acids, well persistence (biofilm formation). This study demonstrated background dating more than years, acquisition wide range that allow it circulate different environments: food clinic or making this bacterium pathogen requires rigorous surveillance strategic interventions mitigate potential risks.

Language: Английский

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Genomic insights into extended-spectrum β-lactamase- and plasmid-borne AmpC-producing Escherichia coli transmission between humans and livestock in rural Cambodia

Journal of Medical Microbiology, Journal Year: 2025, Volume and Issue: 74(3)

Published: March 13, 2025

Introduction. The global spread of extended-spectrum cephalosporinase-producing Escherichia coli (producing β -lactamase or plasmid-borne AmpC, hereafter ESC-Ec) is a major public health concern. Whilst extensively studied in high-income countries, the transmission pathways between humans and animals low- middle-income countries (LMICs) remain unclear. In rural Cambodia, asymptomatic carriage dynamics ESC-Ec living close proximity are poorly understood, highlighting need for targeted research this area. Gap statement. An enhanced understanding genetic epidemiology can enable mitigation strategies to reduce burden disease drug-resistant infections LMIC settings. Aim. This study aimed investigate relatedness genotypic antibiotic resistance profiles strains from livestock Cambodia identify patterns antimicrobial (AMR) gene hosts across households villages. Methodology. Faecal samples were collected 307 285 100 near Kampong Cham Province Cambodia. From these samples, 108 subjected whole-genome sequencing. Core genome MLST (cgMLST) phylogenetic analysis determined relationships strains. All screened presence genes plasmids. Results. Human isolates assigned six phylogroups, with phylogroup A being most common (56.5%). identified 50 sequence types (STs), 17 which shared animals, ST155 prevalent. cgMLST revealed 97 distinct (cgST), indicating strain sharing animals. Additionally, AMR showed widespread resistance, bla CTX-M group detected 84.2% isolates. Notably, such as aph(3'')-Ib–sul2 co-occurred 50% Finally, plasmid IncF plasmids 75.9% isolates, likely facilitating hosts. Conclusions. Our findings demonstrate that their transmitted driven by both clonal plasmid-mediated horizontal transfer. These results highlight urgent stewardship infection control mitigate multidrug-resistant pathogens human animal populations.

Language: Английский

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Polyclonal carbapenemase-producing Escherichia coli in Northern Italy: the emergence of NDM-7

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: March 14, 2025

The spread of extended-spectrum beta-lactamase (ESBL)- and carbapenemase-producing Escherichia coli clones in humans, animals, the environment is great concern worldwide. In this study, we characterized four E. (CP-Ec) isolated from human samples. Two isolates ST401, rarely associated with carbapenemase and/or ESBL resistance genes, harbored blaKPC-3 blaVIM-1 respectively, were genetically distant each other. One CP-Ec isolate belonging to ST355, typically found poultry environmental sources not carbapenemases, was positive showed a wide range virulence genes. last strain belonged ST3564, previously described livestock large virulome but no carbapenemase. ST3564 co-harbored blaNDM-7 which, our knowledge, have been reported Italy. These results emphasize crucial role genomic-based surveillance program intercept emergence critical clones.

Language: Английский

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Antimicrobial Resistance and One Health: Companion Animals as Reservoirs of Bacteria and Resistance Genes in Brazil

Revista de Gestão Social e Ambiental, Journal Year: 2025, Volume and Issue: 19(3), P. e011721 - e011721

Published: March 25, 2025

Objective: This study investigates the role of companion animals, such as dogs and cats, in spread antimicrobial resistance (AMR) Brazil, aiming to understand interaction between humans, environment dissemination resistant bacteria within "One Health" approach. Theoretical Framework: The approach underpins investigation AMR, emphasizing interdependence human, animal, environmental health. Notably, zoonotic pathogens Escherichia coli producing ESBL (extended-spectrum β-lactamase) methicillin-resistant Staphylococcus aureus (MRSA) is a consequence excessive use antimicrobials humans animals. Method: an integrative literature review, searching for articles published last five years (2020 2025) PubMed ScienceDirect databases. search strategy used Health Sciences Descriptors (DeCS): "cats", "dogs", "antimicrobial resistance", "MRSA", "ESBL", "Brazil", combined with boolean operator "AND". Article selection followed relevance methodological quality criteria. Results Discussion: results demonstrated that animals significant, despite being underestimated. main identified were E. MRSA, whose associated pet owners their discussion contextualizes these findings approach, highlighting need preventive measures rational both human veterinary sectors. Research Implications: reinforce importance infection control protocols hospital environments, well guidelines responsible antimicrobials. research also contributes formulation public policies aimed at mitigating AMR from integrated animal health perspective. Originality/Value: by topic still underexplored Brazil. Its lies integrating scientific evidence concept, promoting strategies minimize impacts on

Language: Английский

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Commensal Fitness Advantage May Contribute to the Global Dissemination of Multidrug-Resistant Lineages of Bacteria—The Case of Uropathogenic E. coli

Pathogens, Journal Year: 2023, Volume and Issue: 12(9), P. 1150 - 1150

Published: Sept. 10, 2023

It is widely accepted that favorable fitness in commensal colonization one of the prime facilitators clonal dissemination bacteria. The question arises as to what kind advantage may be wielded by uropathogenic strains two predominant fluoroquinolone- and multidrug-resistant groups E. coli—ST131-H30 ST1193, which has permitted their unprecedented pandemic-like global expansion last few decades. colonization-associated genes’ content, carriage low-cost plasmids, integrons with weak promoters could certainly contribute pandemic groups, although those genetic factors are common among other well. Also, ST131-H30 ST1193 harbor fluoroquinolone-resistance conferring mutations targeting serine residues DNA gyrase (GyrA-S83) topoisomerase IV (ParC-S80) that, backgrounds, might result a benefit, i.e., beyond antibiotic resistance per se. This gain have contributed not only widespread these major clones healthcare setting but also long-term healthy individuals and, thus, circulation community, even low or no fluoroquinolone use environment. evolutionary shift affecting coli, initiated co-favorable both antibiotics-treated patients warrants more in-depth studies monitor further changes epidemiological situation develop effective measures reduce spread.

Language: Английский

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Anthropogenic contamination sources drive differences in antimicrobial-resistant Escherichia coli in three urban lakes

Applied and Environmental Microbiology, Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 13, 2024

A One Health perspective is crucial to understand the extent of antimicrobial resistance (AMR) globally, and investigation AMR in environment has been increasing recent years. However, most studies have focused on waterways that are directly polluted by sewage, industrial manufacturing, or agricultural activities. Therefore, there remains a lack knowledge about more natural, less overtly impacted environments. Through phenotypic genotypic Escherichia coli , this study adds our understanding patterns these types environments, including over time series, showed complex biotic abiotic factors contribute observed. Our further emphasizes importance incorporating surveillance microbes freshwater environments order better comprehend potential risks for both human animal health how may serve as sentinel future clinical infections.

Language: Английский

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Global trends in nonsusceptibility rates of Escherichia coli isolates to meropenem and ceftazidime/avibactam: Data from the Antimicrobial Testing Leadership and Surveillance (ATLAS) programme, 2014–21

International Journal of Antimicrobial Agents, Journal Year: 2024, Volume and Issue: 63(4), P. 107103 - 107103

Published: Feb. 6, 2024

Language: Английский

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Unveiling the High Diversity of Clones and Antimicrobial Resistance Genes in Escherichia coli Originating from ST10 across Different Ecological Niches

Antibiotics, Journal Year: 2024, Volume and Issue: 13(8), P. 737 - 737

Published: Aug. 6, 2024

In this pioneering in silico study Peru, we aimed to analyze

Language: Английский

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Fecal Shedding of Multidrug Resistant Escherichia coli Isolates in Dogs Fed with Raw Meat-Based Diets in Brazil

Antibiotics, Journal Year: 2022, Volume and Issue: 11(4), P. 534 - 534

Published: April 17, 2022

The practice of feeding dogs raw meat-based diets (RMBDs) is growing in several countries, and the risks associated with ingestion pathogenic antimicrobial-resistant Escherichia coli fed these are largely unknown. We characterized E. strains isolated from either an RMBD or a conventional dry feed, according to phylogroup, virulence genes, antimicrobial susceptibility profiles bacteria. Two hundred sixteen were isolated. Dogs RMBDs shed phylogroup E more frequently positive for heat-stable enterotoxin 1-encoding gene. Isolates RMBD-fed also multidrug-resistant isolates including extended-spectrum beta-lactamase (ESBL) producers. Whole-genome sequencing seven ESBL-producing revealed that they predominantly harbored blaCTX-M-55, two colistin-resistant gene mcr-1. These results suggest can affect dog's microbiota, change frequency certain phylogroups, increase shedding diarrheagenic coli. Also, seemed be linked fecal coli, spread harboring mobilizable colistin resistance ESBL genes. This finding concern both animal human health.

Language: Английский

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