Microbiome Analysis of Area in Proximity to White Spot Lesions Reveals More Harmful Plant Pathogens in Maize DOI Creative Commons

Sauban Musa Jibril,

Yanping Hu,

Kexin Yang

et al.

Biomolecules, Journal Year: 2025, Volume and Issue: 15(2), P. 252 - 252

Published: Feb. 9, 2025

Plant microbiomes play a major role in plant health, growth, and development, enhancing resistance to pathogen invasion. However, despite the extensive research on phyllosphere microbiome, it remains unclear how microbiome of leaves proximity diseased responds We investigate response maize white spot by assessing dynamics associated with portion area using 16S ITS high-throughput sequencing analysis. Our results showed that bacterial diversities were higher than those healthy plants. At same time, lower fungal diversity was recorded compared portions leaves. The had significant influence microbial composition. portion, it, dominated genera Sphingomonas, Delftia, Chryseobacterium, Stenotrophomonas, Methylobacterium-methylorubrum, Bacteroides. Still, abundance Sphingomonas decreased corresponding increase Stenotrophomonas. Conversely, genus Setophoma while pathogens Cladosporium, Alternaria, Exserohilum highly abundant samples from it. In addition, co-occurrence network analysis revealed complex infected portion. This study suggests leaf disease is colonized more harmful pathogenic fungi for progression.

Language: Английский

Characterization of a broad-spectrum antifungal strain, Streptomyces graminearus STR-1, against Magnaporthe oryzae DOI Creative Commons

Wenyuan Shen,

Renju Liu, Jiazheng Wang

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: April 8, 2024

Fungal diseases such as the devastating rice blast pose severe threats to crop production worldwide. Biological control of caused by fungal pathogens is an environment-friendly approach for safeguarding production. But insufficient availability microbial agents effective against various has hampered development green in crops. In this study, we identified a broad-spectrum antifungal bacterium, Streptomyces graminearus STR-1, showing antagonistic activity diverse including Magnaporthe oryzae , Rhizoctonia solani Fusarium graminearum Ustilaginoidea virens and Bipolaris maydis . Its was relatively stable less affected temperature pH. Evaluation biocontrol STR-1 revealed that prevented controlled disease via eliciting plant immunity suppressing infection-structure development. broth extract inhibited spore germination, likely through inhibiting protein synthesis. Combining LC–MS chromatography analysis antimicrobial compounds purified from extract, together with decoding genomic sequence, 4-oxo-4-[(1-phenylethyl)amino]but-2-enoic acid, 1,3,5-Trimethylpyrazole SMA-1 potential main secondary metabolites associated its effects. This study suggests bacterial strain could be used identifying highly containing other diseases. The application active offers promising measure tackle disease.

Language: Английский

Citations

6
Elucidating the impact of biochar with different carbon/nitrogen ratios on soil biochemical properties and rhizosphere bacterial communities of flue-cured tobacco plants DOI Creative Commons

Yingfen Yang,

Chenghu Ye,

Wei Zhang

et al.

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: Sept. 15, 2023

In agriculture, biochar (BC) and nitrogen (N) fertilizers are commonly used for improving soil fertility crop productivity. However, it remains unclear how different levels of BC N fertilizer affect productivity.This study elucidates the impact application rates (0, 600, 1200 kg/ha) (105 126 on biomass accumulation, microbial carbon (SMC) (SMN), biochemical properties, including organic (SOC), total (TN), nitrate (NO3--N), ammonium (NH4+-N), urease (UE), acid phosphatase (ACP), catalase (CAT), sucrase (SC) tobacco plants. addition, a high throughput amplicon sequencing technique was adopted to investigate effect BC/N rhizosphere bacterial communities plants.The results confirm that dosages (B1200N126) significantly enhance dry matter accumulation by 31.56% 23.97% compared with control B0N105 B0N126 under field conditions 23.94% 24.52% pot experiment, respectively. The SMC, SMN improved rate (B1200N126), while negatively influenced carbon/nitrogen ratio. Analysis bacteriome through 16S rRNA revealed structure, diversity, composition dramatically changed ratios. Proteobacteria, Bacteroidetes, Actinobacteria, Firmicutes, Acidobacteria were highly abundant phyla in plants treatments. Co-occurrence network analysis displayed fewer negative correlations among than other treatments, which showed less competition resources microbes. redundancy further proved significant positive correlation SMN, dosage (B1200N126).Thus, we conclude (1200 (126 enhances properties activities communities.

Language: Английский

Citations

15
Ralstonia solanacearum differentially modulates soil physicochemical properties and rhizospheric bacteriome of resistant and susceptible tobacco cultivars DOI Creative Commons
Waqar Ahmed,

Zhenlin Dai,

Jinhao Zhang

et al.

Microbiological Research, Journal Year: 2024, Volume and Issue: 281, P. 127604 - 127604

Published: Jan. 10, 2024

Language: Английский

Citations

6
One stone two birds: Endophytes alleviating trace elements accumulation and suppressing soilborne pathogen by stimulating plant growth, photosynthetic potential and defense related gene expression DOI
Muhammad Imran, Zhongke Sun, Kamal A. M. Abo‐Elyousr

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 476, P. 135084 - 135084

Published: July 4, 2024

Language: Английский

Citations

6
Evaluation of the novel endophytic fungus Chaetomium ascotrichoides 1‐24‐2 from Pinus massoniana as a biocontrol agent against pine wilt disease caused by Bursaphelenchus xylophilus DOI
Md Kamaruzzaman, Lijun Zheng, Shun Zhou

et al.

Pest Management Science, Journal Year: 2024, Volume and Issue: 80(10), P. 4924 - 4940

Published: June 11, 2024

Abstract BACKGROUND Bursaphelenchus xylophilus , the causative agent of pine wilt disease (PWD), is an ever‐increasing threat to Pinus forests worldwide. This study aimed develop biological control PWD by application endophytic fungi isolated from healthy trees. RESULTS We successfully a novel fungal strain 1‐24‐2 branches massoniana . The culture filtrates (CFs) exhibited strong nematicidal activity against xylophilus, with corrected mortality rate 99.00%. Based on morphological and molecular characteristics, was identified as Chaetomium ascotrichoides In in‐planta assay, seedlings (2‐years‐old) treated CFs + wood nematode (T2) showed significant effect 80%. A total 24 toxic compounds were first through gas chromatography–mass spectrometry (GC–MS) analysis, which O ‐methylisourea, 2‐chlorobenzothiazole, 4,5,6‐trihydroxy‐7‐methylphthalide robust binding sites at Tyr119 phosphoethanolamine methyltransferase (PMT) protein docking approach could be used potential for developing effective nematicides. Interestingly, produces volatile organic (VOCs), disturb natural development process B. whose number decreased up 83.32% in treatment group compared also reduced Botrytis cinerea growth 71.01%. CONCLUSION Our results highlight C. promising biocontrol solid report P. exhibiting world. © 2024 Society Chemical Industry.

Language: Английский

Citations

5
Biocontrol potential of plant growth-promoting rhizobacteria against plant disease and insect pest DOI

Qinhao Jian,

Tongrui Zhang,

Yingying Wang

et al.

Antonie van Leeuwenhoek, Journal Year: 2024, Volume and Issue: 117(1)

Published: July 1, 2024

Language: Английский

Citations

4
Transcriptome profiling reveals the impact of various levels of biochar application on the growth of flue-cured tobacco plants DOI Creative Commons

Yingfen Yang,

Waqar Ahmed, Gang Wang

et al.

BMC Plant Biology, Journal Year: 2024, Volume and Issue: 24(1)

Published: July 10, 2024

Abstract Background Biochar, a carbon-rich source and natural growth stimulant, is usually produced by the pyrolysis of agricultural biomass. It widely used to enhance plant growth, enzyme activity, crop productivity. However, there are no conclusive studies on how different levels biochar application influence these systems. Methods results The present study elucidated dose-dependent effects physiological performance, dry matter accumulation tobacco plants via field experiments. In addition, transcriptome analysis was performed 60-day-old (early stage) 100-day-old (late leaves determine changes in transcript at molecular level under various (0, 600, 1800 kg/ha). demonstrated that optimum enhances regulates enzymatic promotes biomass plants, while higher doses had adverse effects. Furthermore, revealed total 6561 differentially expressed genes (DEGs) were up- or down-regulated groupwise comparison treatments. KEGG pathways carbon fixation photosynthetic organisms (ko00710), photosynthesis (ko00195), starch sucrose metabolism (ko00500) significantly up-regulated optimal dosage (600 kg/ha) (1800 Conclusion Collectively, indicate an rate could positively affect fixation, which turn increased synthesis starch, thus promoting plants. disturbs crucial source-sink balance organic compounds inhibits

Language: Английский

Citations

4
Increasing Application of Multifunctional Bacillus for Biocontrol of Pests and Diseases and Plant Growth Promotion: Lessons from Brazil DOI Creative Commons
Natalia Caetano Vasques, Marco Antônio Nogueira, Mariangela Hungría

et al.

Agronomy, Journal Year: 2024, Volume and Issue: 14(8), P. 1654 - 1654

Published: July 27, 2024

The microbial genus Bacillus inhabits a diverse range of environments and is widespread across all global biomes, with significant presence in soil habitats. In agriculture, strains play multifaceted roles, serving as biocontrol agents against pests diseases, promoting plant growth by facilitating nutrient availability enhancing stress tolerance. Through mechanisms such phosphate solubilization, ACC-deaminase activity, synthesis phytohormones siderophores, spp. contribute to health crop productivity, new approach regenerative agriculture. ability solubilize makes essential nutrients more accessible plants, while activity helps plants withstand environmental stresses. Additionally, the can stimulate development, siderophores may facilitate uptake iron plants. As agricultural industry embraces Bacillus-based formulations for pest management enhancement, future research holds promising prospects optimizing their applications harnessing full potential agroecosystems. Continued exploration diversity interactions microbiota will further advance sustainable practices. This review contributes understanding how revolutionize agriculture health, increasing providing effective biological solutions diseases. successful application technologies millions hectares Brazilian demonstrates synergy between need practices use bio-inputs.

Language: Английский

Citations

4
Plant microRNAs regulate the defense response against pathogens DOI Creative Commons
Changxin Luo, Nawaz Haider Bashir,

Zhumei Li

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 30, 2024

MicroRNAs (miRNAs) are a class of small non-coding RNAs, typically 20-25 nucleotides in length, that play crucial role regulating gene expression post-transcriptionally. They involved various biological processes such as plant growth, development, stress response, and hormone signaling pathways. Plants interact with microbes through multiple mechanisms, including mutually beneficial symbiotic relationships complex defense strategies against pathogen invasions. These encompass physical barriers, biochemical defenses, signal recognition transduction, well systemic acquired resistance. MiRNAs central the plant's innate immune activating or suppressing transcription specific genes directly mechanisms pathogens. Notably, miRNAs respond to attacks by modulating balance hormones salicylic acid, jasmonic ethylene, which key mechanisms. Moreover, can cross boundaries into fungal bacterial cells, performing cross-kingdom RNA silencing enhances disease Despite diverse roles defense, further research their function plant-pathogen interactions is essential. This review summarizes critical pathogens, for elucidating how control

Language: Английский

Citations

4
Metagenomic Analysis of Garden Soil-Derived Microbial Consortia and Unveiling Their Metabolic Potential in Mitigating Toxic Hexavalent Chromium DOI Creative Commons
Nidhi Singh, Veer Singh, Sachchida Nand

et al.

Life, Journal Year: 2022, Volume and Issue: 12(12), P. 2094 - 2094

Published: Dec. 13, 2022

Soil microbial communities connect to the functional environment and play an important role in biogeochemical cycle waste degradation. The current study evaluated distribution of core population garden soil Varanasi region Uttar Pradesh, India their metabolic potential for mitigating toxic hexavalent chromium from wastewater. Metagenomes contain 0.2 million reads 56.5% GC content. metagenomic analysis provided insight into relative abundance revealed domination around 200 bacterial species belonging different phyla four archaeal phyla. top 10 abundant genera were Gemmata, Planctomyces, Steroidobacter, Pirellula, Pedomicrobium, Rhodoplanes, Nitrospira Mycobacterium, Pseudonocardia, Acinetobacter. In this study, Gemmata was dominating genera. Euryarchaeota, Parvarchaeota, Crenarchaeota present with low samples. X-ray photoelectric spectroscopy (XPS) indicates presence carbon, nitrogen–oxygen, calcium, phosphorous, silica soil. Soil-derived consortia showed high [Cr (VI)] removal efficiency (99.37%). isolated had bioremediation, thus, could be beneficial design a heavy-metal treatment system.

Language: Английский

Citations

17