Disarming Staphylococcus aureus: Review of Strategies Combating This Resilient Pathogen by Targeting Its Virulence

Pathogens, Journal Year: 2025, Volume and Issue: 14(4), P. 386 - 386

Published: April 15, 2025

Staphylococcus aureus is a formidable pathogen notorious for its antibiotic resistance and diverse virulence mechanisms, including toxin production, biofilm formation, immune evasion. This article explores innovative anti-virulence strategies to disarm S. by targeting critical factors without exerting bactericidal pressure. Key approaches include inhibiting adhesion neutralizing toxins, disrupting quorum sensing (e.g., Agr system inhibitors), blocking iron acquisition pathways. Additionally, interventions two-component regulatory systems are highlighted. While promising, challenges such as strain variability, resilience, pharmacokinetic limitations, evolution underscore the need combination therapies advanced formulations. Integrating with traditional antibiotics host-directed offers sustainable solution combat multidrug-resistant aureus, particularly methicillin-resistant strains (MRSA), mitigate global public health crisis.

Language: Английский

Drug repurposing: insights into the antimicrobial effects of AKBA against MRSA

AMB Express, Journal Year: 2024, Volume and Issue: 14(1)

Published: Jan. 6, 2024

Staphylococcus aureus is a major threat in infectious diseases due to its varied infection types and increased resistance. S. could form persister cells under certain condition also attach on medical apparatus biofilms, which exhibited extremely high resistance antibiotics. 3-Acetyl-11-keto-beta-boswellic acid (AKBA) well-studied anti-tumor antioxidant drug. This study aimed determine the antimicrobial effects of AKBA against biofilms. The vitro susceptibility was assessed by micro-dilution assay, disc diffusion assay time-killing assay. Drug combination between conventional antibiotics detected checkerboard And antibiofilm were explored crystal violet staining combined with SYTO/PI probes staining. Next, RBC lysis activity CCK-8 kit used cytotoxicity AKBA. In addition, murine subcutaneous abscess model assess vivo. Our results revealed that found show effective methicillin-resistant (MRSA) minimal inhibitory concentration 4-8 µg/mL undetectable cytotoxicity. no resistant mutation induced after 20 days consecutive passage. Further, we be synergy gentamycin or amikacin clinical isolates. By SYTO9/PI staining, strong biofilm eradication at 1 ~ 4 µg/mL. effect verified vivo mouse model. detectable toxicity found. These indicated has great potential development as an alternative treatment for refractory infections.

Language: Английский

Nanocomposite system with photoactive phloxine B eradicates resistant Staphylococcus aureus

Heliyon, Journal Year: 2024, Volume and Issue: 10(13), P. e33660 - e33660

Published: June 26, 2024

Language: Английский