Challenges in the Biotechnological Implementation of Oral RNA Interference as an Antiviral Strategy inAedes aegypti DOI Creative Commons
Ottavia Romoli,

Annabelle Henrion-Lacritick,

Hervé Blanc

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 5, 2023

Abstract Mosquitoes, particularly Aedes aegypti , are critical vectors for globally significant pathogenic viruses. This study examines the limitations of oral RNA interference (RNAi) as a strategy to disrupt viral transmission by Ae. . We hypothesized that double-stranded (dsRNA) targeting Zika virus (ZIKV) or chikungunya (CHIKV) genomes produced engineered bacterial symbionts could trigger an antiviral response. Mosquitoes mono-colonized with Escherichia coli producing dsZIK dsCHIK did not display reduced titers following exposure virus-contaminated bloodmeals and failed generate dsZIK- dsCHIK-derived small interfering RNAs. To address potential dsRNA release, we explored inoculation via feeding injection. While replication was impeded in mosquitoes injected dsCHIK, no effect observed dsRNA-fed mosquitoes. These findings highlight complexities implementing RNAi warrant further exploration local systemic mechanisms.

Language: Английский

Trends and emerging hotspots in RNAi-based arthropod pest control: A comprehensive bibliometric analysis DOI
Wenbin Liu, Xinyu Wang,

Anmo Zhou

et al.

Journal of Insect Physiology, Journal Year: 2025, Volume and Issue: 161, P. 104754 - 104754

Published: Feb. 9, 2025

Language: Английский

Citations

0
Symbiont-Mediated RNAi: Mechanisms, Current Status and Prospects DOI
Marcela B. Figueiredo, Miranda M. A. Whitten, Paul Dyson

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Plastid-Mediated RNA Interference for Pest Control DOI
Shengchun Li, Mengting Wu, Jiang Zhang

et al.

Published: Jan. 1, 2025

Language: Английский

Citations

0
Limitations in harnessing oral RNA interference as an antiviral strategy in Aedes aegypti DOI Creative Commons
Ottavia Romoli,

Annabelle Henrion-Lacritick,

Hervé Blanc

et al.

iScience, Journal Year: 2024, Volume and Issue: 27(3), P. 109261 - 109261

Published: Feb. 16, 2024

Mosquitoes, particularly

Language: Английский

Citations

2
Functions and regulations of insect gut bacteria DOI
Shuncai Han, Muhammad Akhtar, Xiaofeng Xia

et al.

Pest Management Science, Journal Year: 2024, Volume and Issue: 80(10), P. 4828 - 4840

Published: June 17, 2024

Abstract The insect gut is a complicated ecosystem that inhabits large number of symbiotic bacteria. As an important organ the host insect, bacteria play very roles in regulating physiological and metabolic processes. Recently, much progress has been made study guts with development high‐throughput sequencing technology molecular biology. This review summarizes primary functions guts, such as enhancing insecticide resistance, facilitating food digestion, promoting detoxification, mating behavior egg hatching. It also addresses some possible pathways symbiont regulation governed by external habitats, conditions immunity insect. provides solid foundations for further studies on novel theories, new technologies practical applications guts. © 2024 Society Chemical Industry.

Language: Английский

Citations

1
Combination of computational techniques and RNAi reveal targets in Anopheles gambiae for malaria vector control DOI Creative Commons
Eunice O. Adedeji,

Thomas Beder,

Claudia Damiani

et al.

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(7), P. e0305207 - e0305207

Published: July 5, 2024

Increasing reports of insecticide resistance continue to hamper the gains vector control strategies in curbing malaria transmission. This makes identifying new targets or alternative necessary. CLassifier Essentiality AcRoss EukaRyote (CLEARER), a leave-one-organism-out cross-validation machine learning classifier for essential genes, was used predict genes Anopheles gambiae and selected predicted experimentally validated. The CLEARER algorithm trained on six model organisms: Caenorhabditis elegans , Drosophila melanogaster Homo sapiens Mus musculus Saccharomyces cerevisiae Schizosaccharomyces pombe employed identify An . Of 10,426 1,946 (18.7%) were be Cellular Essential Genes (CEGs), 1716 (16.5%) Organism (OEGs), 852 (8.2%) as both OEGs CEGs. RNA interference (RNAi) validate top three highly expressed non-ribosomal predictions probable targets, by determining effect these survival G3 mosquitoes. In addition, knockdown arginase (AGAP008783) Plasmodium berghei infection mosquitoes evaluated, an enzyme we computationally inferred earlier based chokepoint analysis. Arginase AGAP007406 (Elongation factor 1-alpha, Elf1), AGAP002076 (Heat shock 70kDa protein 1/8, HSP), AGAP009441 2, Elf2), had efficiencies 91%, 75%, 63%, 61%, respectively. While HSP Elf2 significantly reduced longevity (p<0.0001) compared groups, Elf1 no survival. However, P oocytes counts midgut when LacZ-injected controls. study reveals important contributors mosquito parasite development, hence placing them possible control.

Language: Английский

Citations

1
Challenges in the Biotechnological Implementation of Oral RNA Interference as an Antiviral Strategy inAedes aegypti DOI Creative Commons
Ottavia Romoli,

Annabelle Henrion-Lacritick,

Hervé Blanc

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Nov. 5, 2023

Abstract Mosquitoes, particularly Aedes aegypti , are critical vectors for globally significant pathogenic viruses. This study examines the limitations of oral RNA interference (RNAi) as a strategy to disrupt viral transmission by Ae. . We hypothesized that double-stranded (dsRNA) targeting Zika virus (ZIKV) or chikungunya (CHIKV) genomes produced engineered bacterial symbionts could trigger an antiviral response. Mosquitoes mono-colonized with Escherichia coli producing dsZIK dsCHIK did not display reduced titers following exposure virus-contaminated bloodmeals and failed generate dsZIK- dsCHIK-derived small interfering RNAs. To address potential dsRNA release, we explored inoculation via feeding injection. While replication was impeded in mosquitoes injected dsCHIK, no effect observed dsRNA-fed mosquitoes. These findings highlight complexities implementing RNAi warrant further exploration local systemic mechanisms.

Language: Английский

Citations

0