Bacterial Community Composition and Prevalence of Aeromonas dhakensis in Four Tilapia Freshwater Aquaculture Systems in Malaysia DOI Creative Commons

Sook Ling Lim,

Suat Moi Puah,

Siti Nursyuhada Baharudin

et al.

Fishes, Journal Year: 2025, Volume and Issue: 10(5), P. 204 - 204

Published: May 1, 2025

The tilapia (Oreochromis) aquaculture industry in Malaysia has expanded rapidly to meet the increasing demand for animal protein. However, this growth is challenged by microbial infections, particularly those caused emerging pathogen Aeromonas dhakensis. This study aimed investigate community composition across four distinct farming systems and assess associated water physicochemical parameters, with a focus on detecting presence of A. dhakensis elucidating its environmental associations. Water parameters were measured evaluate conditions, communities characterized through 16S rDNA metabarcoding, was detected using both microbiological molecular approaches. Principal component analysis (PCA) canonical correspondence (CCA) employed explore influence variables dynamics occurrence. Our results indicated that floating cages exhibited higher levels temperature, ammonium, fecal coliform, while cement tanks showed signs nutrient accumulation. PCA revealed degraded quality. A total 45 strains fingerprints isolated. metabarcoding Proteobacteria, Actinobacteria, Planctomycetota as dominant phyla. Alpha diversity did not differ significantly among pond systems, beta variations assemblages systems. CCA identified dissolved oxygen, macronutrients (phosphate, nitrate, nitrite), turbidity significant drivers shaping structure In conclusion, highlights importance factors, levels, potentially influencing pathogenic bacteria such These findings underscore need improved management mitigate disease risks support fish health.

Language: Английский

Bacterial Community Composition and Prevalence of Aeromonas dhakensis in Four Tilapia Freshwater Aquaculture Systems in Malaysia DOI Creative Commons

Sook Ling Lim,

Suat Moi Puah,

Siti Nursyuhada Baharudin

et al.

Fishes, Journal Year: 2025, Volume and Issue: 10(5), P. 204 - 204

Published: May 1, 2025

The tilapia (Oreochromis) aquaculture industry in Malaysia has expanded rapidly to meet the increasing demand for animal protein. However, this growth is challenged by microbial infections, particularly those caused emerging pathogen Aeromonas dhakensis. This study aimed investigate community composition across four distinct farming systems and assess associated water physicochemical parameters, with a focus on detecting presence of A. dhakensis elucidating its environmental associations. Water parameters were measured evaluate conditions, communities characterized through 16S rDNA metabarcoding, was detected using both microbiological molecular approaches. Principal component analysis (PCA) canonical correspondence (CCA) employed explore influence variables dynamics occurrence. Our results indicated that floating cages exhibited higher levels temperature, ammonium, fecal coliform, while cement tanks showed signs nutrient accumulation. PCA revealed degraded quality. A total 45 strains fingerprints isolated. metabarcoding Proteobacteria, Actinobacteria, Planctomycetota as dominant phyla. Alpha diversity did not differ significantly among pond systems, beta variations assemblages systems. CCA identified dissolved oxygen, macronutrients (phosphate, nitrate, nitrite), turbidity significant drivers shaping structure In conclusion, highlights importance factors, levels, potentially influencing pathogenic bacteria such These findings underscore need improved management mitigate disease risks support fish health.

Language: Английский

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