A decade of genomic and phenotypic adaptation of carbapenem-resistant Acinetobacter baumannii DOI Creative Commons

Astri D. Tagueha,

Cartesio D’Agostini, Daniela Scribano

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: April 30, 2025

Introduction Acinetobacter baumannii exhibits high genomic plasticity, enabling it to acquire virulence factors and antibiotic resistance (AR). Understanding its evolutionary adaptations is crucial for developing effective therapeutic strategies. Methods Thirty clinical isolates collected from two distinct time periods, defined as older (2010–2013), recent (2022–2023),- were compared phenotypically (antibiotic resistance, growth, biofilm formation, desiccation tolerance, invasiveness) genotypically (whole-genome sequencing). Results All displayed an extensively drug-resistant phenotype. Overall, respiratory harbored a higher content of antibiotic-resistant genes (ARGs), with showing 12.5% increases in the average number ARGs urine ( P = 0.02). More than 50% strains faster stronger increased lung cell invasiveness isolates, while over 70% showed greater tolerance bladder invasiveness. Eleven factor shared between old eight common urinary no overlap among isolates. Statistically significant positive correlations observed fast-growing strong biofilm-forming well their Conversely, negative found collection time, isolation site, host Analysis macrocolony types revealed link phenotypic behavior. Conclusion Significant genetic variability was past Older had more involved adhesion nutrient uptake, demonstrated formation invasiveness, reflecting adaptation pressures. These findings highlight dynamic evolution A. , providing insights future strategies infection control.

Language: Английский

Understanding the clinical and molecular epidemiological characteristics of carbapenem-resistant Acinetobacter baumannii infections within intensive care units of three teaching hospitals DOI Creative Commons
Pengyu Zhang,

Jingchen Hao,

Yafen Zhang

et al.

Annals of Clinical Microbiology and Antimicrobials, Journal Year: 2025, Volume and Issue: 24(1)

Published: Jan. 13, 2025

Carbapenem-resistant Acinetobacter baumannii (CRAB) is recognized as a common clinical conditional pathogen with blaOXA-23 gene-mediated multidrug-resistance that significant threat to public health safety. Timely and effective infection control measures are needed prevent their spread. We conducted retrospective study of CRAB patients at three teaching hospitals from 2019 2022. identified bacterial isolates, collected data, performed antimicrobial susceptibility testing. Genome characteristics isolates were investigated by whole genome sequencing. Multilocus sequence typing phylogenetic trees used assess the genetic similarity isolates. Acquired resistance genes virulence factors carried in isolated group analyzed ResFinder, PubMLST VFDB. Sequence alignment was analyze environment around blaOXA-23. Phylogenetic tree constructed relationship A total 92 non-repetitive collected, sputum samples accounting for majority (94.57%, n = 87) samples. These distributed into ST2, ST2 have highest prevalence infection, 99.99% (n 91) all The major blaOXA-23, blaOXA-66, blaOXA-51, blaADC. Also, strains showed high levels antibiotics, but not minocycline. Meanwhile, most such various ompA, csuA, csuB, csuC, csuD, abaI, abaR, lpxC, lpxA, bmfRS. Single nucleotide polymorphism (SNP) analyses further indicated progressively polymorphic time. gene found accumulated context prominent environmental antibiotic exposure had longer survival times environment, resulting tendency bacteria develop greater resistance. find prevalent within ICU resistant antibiotics over Enhanced understanding timely management infections will be crucial minimize or even eliminate spread setting.

Language: Английский

Citations

0
Mitigating the global spread of multidrug-resistant Acinetobacter buamanni in Immunocompromised Patients DOI Creative Commons
Maria Qadri,

Hira Tariq,

Muhammad Shahid Mehmood

et al.

Deleted Journal, Journal Year: 2025, Volume and Issue: 22(1)

Published: Feb. 17, 2025

Language: Английский

Citations

0
Exploring the clinical outcomes and molecular characteristics of Acinetobacter baumannii bloodstream infections: a study of sequence types, capsular types, and drug resistance in China DOI Creative Commons

Jiao Chen,

Yanting Shao, Zhibin Cheng

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: Feb. 17, 2025

Bloodstream infections (BSIs) caused by Acinetobacter baumannii have been associated with high mortality. To improve the outcomes of patients, this study explored clinical characteristics and patients BSIs, as well phenotypic genomic these isolates. A retrospective cohort was conducted involving A. BSIs cases from 2020 to 2023 in a tertiary hospital. The all isolates were evaluated. Virulence phenotypes evaluated using growth curve, biofilm-forming assay, antiserum complement killing, G.mellonella killing assay. Furthermore, whole-genome sequencing (WGS) utilized analyze characteristics. 30-day mortality rate 67 55.22%. Patients death group had significantly lower platelet counts higher CRP levels than those survival group. Additionally, rates antibiotic use (≥2 classes) greater carbapenem exposure observed. Among isolates, CRAb accounted for 80.6%, ST2 76.12%, KL2/3/7/77/160 65.67%. predominant KL type KL3, found 19.4% All CRAb. 90.7% coharbored blaOXA-23 blaOXA-66 , while one blaNDM-1 . Compared non-ST2 non infections, (66.0% vs. 23.5%, P=0.002; 65.90% 34.78%, P=0.015). underwent more invasive procedures, received two or antibiotics therapy before isolation, serum albumin levels. These exhibited resistance antimicrobial agents. No significant differences virulence observed between groups, except biofilm formation groups (P=0.002). However, harbored genes related iron uptake formation. is high. It great significance clinicians pay attention risk factors identify ST types strains causing infection at an early stage.

Language: Английский

Citations

0
Large-scale genomic analysis reveals significant role of insertion sequences in antimicrobial resistance of Acinetobacter baumannii DOI Creative Commons
Fei Xie, Lifeng Wang, Song Li

et al.

mBio, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 20, 2025

ABSTRACT Acinetobacter baumannii , a prominent nosocomial pathogen renowned for its extensive resistance to antimicrobial agents, poses significant challenge in the accurate prediction of (AMR) from genomic data. Despite thorough researches on molecular mechanisms AMR, gaps remain our understanding key contributors. This study utilized rule-based and three machine learning models predict AMR phenotypes, aiming decipher factors associated with AMR. Genomes antibiotic phenotypes 1,012 public isolates were employed model construction training. To validate models, data set comprising 164 self-collected strains underwent next-generation sequencing, nanopore long-read susceptibility testing using broth dilution method. It was found that presence genes (ARGs) alone insufficient accurately phenotype majority antibiotics (90%, 18 out 20) set. Conversely, it observed combining ARGs insertion sequence (IS) elements significantly enhanced predictive performance. The Random Forest outperform support vector (SVM), logistic regression model, method across all 20 antibiotics, accuracies ranging 83.80% 97.70%. In validation set, even higher achieved, 85.63% 99.31%. Furthermore, conserved patterns between IS validated sequencing data, substantially enhancing accuracy A. . underscores pivotal role IMPORTANCE interplay sequences (ISs) contributes against specific antibiotics. Conventionally, genetic variations have been predicting potential largely overlooked. Our advances this approach by integrating both enhances prediction, emphasizing function resistance. Notably, we uncover series linking ARGs, which phenotypic prediction. findings are crucial bioinformatics strategies aimed at studying tracking offering novel insights into combating escalating challenge.

Language: Английский

Citations

0
Genotypic diversity and antimicrobial resistance phenotype of carbapenem-resistant and carbapenem-susceptible Acinetobacter species isolates DOI
Ka Lip Chew,

Keai-Fong Tan,

Nur Aisyah Binte Abu Bakar

et al.

Pathology, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0
Whole genome sequencing and phylogenetic analyses of the Sillaginidae family fish DOI
Qi Liu, Weihua Mao, Yiting Wang

et al.

Molecular Phylogenetics and Evolution, Journal Year: 2025, Volume and Issue: unknown, P. 108340 - 108340

Published: March 1, 2025

Language: Английский

Citations

0
The fitness connection of antibiotic resistance DOI Creative Commons
M. Füzi

Frontiers in Microbiology, Journal Year: 2025, Volume and Issue: 16

Published: April 10, 2025

More than three decades ago multidrug-resistant (MDR) clones of the pathogens: Staphylococcus aureus, Escherichia coli, Klebsiella pneumoniae, Clostridioides difficile, Enterococcus faecium, Pseudomonas aeruginosa and Acinetobacter baumannii have started to disseminate across wide geographical areas. A characteristic feature all these MDR lineages is carriage some mutations in quinolone resistance-determining regions (QRDRs) DNA gyrase topoisomerase IV which besides conferring resistance fluoroquinolones are associated with a fitness benefit. Several lines evidence strongly suggest that extra conferred by facilitated dissemination international lineages. pathogens require energy cover cost excess antibiotic gene cargo. However, generated upgraded metabolic activity was demonstrated increase uptake antibiotics enhancing susceptibility. Accordingly, bacteria need additional positive schemes which, similarly QRDR advantage, will not compromise resistance. Some these, clone-specific effects large genomes, low-cost plasmids, transfer plasmid genes chromosome, application weak promoters integrons various techniques for economic control integrase enzyme including highly sophisticated system A. baumannii. These impacts - among others confer advantage promoting spread pathogens. even potential combined effect without limit virulence-related or less relevant cargoes often be sacrificed permit acquisition high-priority determinants. Accordingly major clone strains usually virulent susceptible isolates. In summary, approach research very useful since status seem profoundly impact capacity healthcare setting.

Language: Английский

Citations

0
A decade of genomic and phenotypic adaptation of carbapenem-resistant Acinetobacter baumannii DOI Creative Commons

Astri D. Tagueha,

Cartesio D’Agostini, Daniela Scribano

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2025, Volume and Issue: 15

Published: April 30, 2025

Introduction Acinetobacter baumannii exhibits high genomic plasticity, enabling it to acquire virulence factors and antibiotic resistance (AR). Understanding its evolutionary adaptations is crucial for developing effective therapeutic strategies. Methods Thirty clinical isolates collected from two distinct time periods, defined as older (2010–2013), recent (2022–2023),- were compared phenotypically (antibiotic resistance, growth, biofilm formation, desiccation tolerance, invasiveness) genotypically (whole-genome sequencing). Results All displayed an extensively drug-resistant phenotype. Overall, respiratory harbored a higher content of antibiotic-resistant genes (ARGs), with showing 12.5% increases in the average number ARGs urine ( P = 0.02). More than 50% strains faster stronger increased lung cell invasiveness isolates, while over 70% showed greater tolerance bladder invasiveness. Eleven factor shared between old eight common urinary no overlap among isolates. Statistically significant positive correlations observed fast-growing strong biofilm-forming well their Conversely, negative found collection time, isolation site, host Analysis macrocolony types revealed link phenotypic behavior. Conclusion Significant genetic variability was past Older had more involved adhesion nutrient uptake, demonstrated formation invasiveness, reflecting adaptation pressures. These findings highlight dynamic evolution A. , providing insights future strategies infection control.

Language: Английский

Citations

0