The Role of the Gut Microbiota in Female Reproductive and Gynecological Health: Insights into Endometrial Signaling Pathways DOI Creative Commons

P. Mora,

Diana Valbuena, Antonio Dı́ez-Juan

et al.

Life, Journal Year: 2025, Volume and Issue: 15(5), P. 762 - 762

Published: May 9, 2025

Fertility is a dynamic, multifactorial process governed by hormonal, immune, metabolic, and environmental factors. Recent evidence highlights the gut microbiota as key systemic regulator of reproductive health, with notable impacts on endometrial function, implantation, pregnancy maintenance, timing birth. This review examines gut–endometrial axis, focusing how microbial communities influence biology through molecular signaling pathways. We discuss modulatory roles microbial-derived metabolites—including short-chain fatty acids, bile tryptophan catabolites—in shaping immune tolerance, estrogen metabolism, epithelial integrity at uterine interface. Emphasis placed shared mechanisms such β-glucuronidase-mediated recycling, Toll-like receptor (TLR)-driven inflammation, Th17/Treg cell imbalance, translocation, which collectively implicate dysbiosis in etiology gynecological disorders including endometriosis, polycystic ovary syndrome (PCOS), recurrent implantation failure (RIF), preeclampsia (PE), preterm birth (PTB). Although most current remains correlational, emerging insights from metagenomic metabolomic profiling, along microbiota-depletion models Mendelian randomization studies, underscore biological significance gut-reproductive crosstalk. By integrating concepts microbiology, immunology, biology, this offers systems-level perspective host–microbiota interactions female fertility.

Language: Английский

Reinvigorating AMR resilience: leveraging CRISPR–Cas technology potentials to combat the 2024 WHO bacterial priority pathogens for enhanced global health security—a systematic review DOI Creative Commons
Olalekan John Okesanya, Mohamed Mustaf Ahmed, Jerico Bautista Ogaya

et al.

Tropical Medicine and Health, Journal Year: 2025, Volume and Issue: 53(1)

Published: April 2, 2025

Abstract Background Antimicrobial resistance (AMR) poses a global health threat, particularly in low- and middle-income countries (LMICs). Clustered regularly interspaced short palindromic repeats (CRISPR)–Cas system technology offers promising tool to combat AMR by targeting disabling genes WHO bacterial priority pathogens. Thus, we systematically reviewed the potential of CRISPR–Cas address AMR. Methods This systematic review adhered Preferred Reporting Items for Systematic Reviews Meta-Analyses (PRISMA) guidelines. A comprehensive literature search was conducted using Scopus PubMed databases, focusing on publications from 2014 June 2024. Keywords included “CRISPR/Cas,” “antimicrobial resistance,” “pathogen.” The eligibility criteria required original studies involving CRISPR/Cas systems that targeted Data were extracted eligible studies, qualitatively synthesized, assessed bias Joanna Briggs Institute (JBI)-standardized tool. Results 48 revealed diverse systems, including CRISPR–Cas9, CRISPR–Cas12a, CRISPR–Cas3, various genes, such as blaOXA-232, blaNDM, blaCTX-M, ermB, vanA, mecA , fosA3 blaKPC mcr-1, which are responsible carbapenem, cephalosporin, methicillin, macrolide, vancomycin, colistin, fosfomycin resistance. Some have explored role CRISPR virulence gene suppression, enterotoxin tsst1 iutA Staphylococcus aureus Klebsiella pneumoniae . Delivery mechanisms include bacteriophages, nanoparticles, electro-transformation, conjugative plasmids, demonstrate high efficiency vitro vivo. CRISPR-based diagnostic applications demonstrated sensitivity specificity, with detection limits low 2.7 × 10 2 CFU/mL, significantly outperforming conventional methods. Experimental reported significant reductions resistant populations complete suppression strains. Engineered phagemid particles plasmid-curing been shown eliminate IncF cured plasmids carrying vanA mcr-1 blaNDM 94% efficiency, restore antibiotic susceptibility. Gene re-sensitization strategies used susceptibility E. coli blaKPC-2-mediated carbapenem MDR bacteria. Whole-genome sequencing bioinformatics tools provided deeper insights into CRISPR-mediated defense mechanisms. Optimization enhanced gene-editing efficiencies, offering approach tackling high-priority Conclusions has across While promising, challenges optimizing vivo delivery, mitigating resistance, navigating ethical-regulatory barriers must be addressed facilitate clinical translation.

Language: Английский

Citations

1
Mechanisms, therapeutic strategies, and emerging therapeutic alternatives for carbapenem resistance in Gram-negative bacteria DOI

Fatima Mourabiti,

Fatimazahra Jouga,

Souraya Sakoui

et al.

Archives of Microbiology, Journal Year: 2025, Volume and Issue: 207(3)

Published: Feb. 13, 2025

Language: Английский

Citations

0
Changes in root-associated bacterial communities across growth stages of salt-tolerant and salt-sensitive rice grown in coastal saline-alkali soils DOI
Qing Li,

Jihui Jiang,

Haoyang Cheng

et al.

World Journal of Microbiology and Biotechnology, Journal Year: 2025, Volume and Issue: 41(3)

Published: Feb. 27, 2025

Language: Английский

Citations

0
The Role of the Gut Microbiota in Female Reproductive and Gynecological Health: Insights into Endometrial Signaling Pathways DOI Creative Commons

P. Mora,

Diana Valbuena, Antonio Dı́ez-Juan

et al.

Life, Journal Year: 2025, Volume and Issue: 15(5), P. 762 - 762

Published: May 9, 2025

Fertility is a dynamic, multifactorial process governed by hormonal, immune, metabolic, and environmental factors. Recent evidence highlights the gut microbiota as key systemic regulator of reproductive health, with notable impacts on endometrial function, implantation, pregnancy maintenance, timing birth. This review examines gut–endometrial axis, focusing how microbial communities influence biology through molecular signaling pathways. We discuss modulatory roles microbial-derived metabolites—including short-chain fatty acids, bile tryptophan catabolites—in shaping immune tolerance, estrogen metabolism, epithelial integrity at uterine interface. Emphasis placed shared mechanisms such β-glucuronidase-mediated recycling, Toll-like receptor (TLR)-driven inflammation, Th17/Treg cell imbalance, translocation, which collectively implicate dysbiosis in etiology gynecological disorders including endometriosis, polycystic ovary syndrome (PCOS), recurrent implantation failure (RIF), preeclampsia (PE), preterm birth (PTB). Although most current remains correlational, emerging insights from metagenomic metabolomic profiling, along microbiota-depletion models Mendelian randomization studies, underscore biological significance gut-reproductive crosstalk. By integrating concepts microbiology, immunology, biology, this offers systems-level perspective host–microbiota interactions female fertility.

Language: Английский

Citations

0