Active herpesviruses are associated with intensive care unit (ICU) admission in patients pulmonary infection and alter the respiratory microbiome DOI Creative Commons
Zhiguang Liu, Chunjian Qi, Yujia Shi

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 9, 2024

The Herpesviridae family contains several human-related viruses, which are able to establish colonizing and latency in the human body, posing a significant threat prognosis of patients. Pulmonary infections represent one predominant infectious diseases globally, characterized by diverse multifaceted clinical manifestations that have consistently attracted clinician's concern. However, relationship herpesviruses on pulmonary respiratory microbiota remains poorly understood.

Language: Английский

Cell-free DNA Levels in Herpes Zoster: A Cross-sectional Longitudi­nal Study DOI Creative Commons

Cathy Gordon,

Yaron Zenaty,

Ayelet Ollech

et al.

Acta Dermato Venereologica, Journal Year: 2025, Volume and Issue: 105, P. adv42137 - adv42137

Published: Jan. 3, 2025

This study investigates serum cell-free DNA fluctuations in patients with herpes zoster or post-herpetic neuralgia, offering insight into the tissue damage and inflammatory dynamics associated these conditions. A single-centre combined cross-sectional longitudinal was conducted 59 to assess levels neuralgia. Cell-free extracted from blood samples of neuralgia compared healthy controls. The findings demonstrated elevated zoster, which remained for 3 months longer following treat-ment. These results suggest presence a subacute state after infection. Furthermore, who developed did not show levels, while those develop exhibited increased levels. indicates that is likely localized response prior nerve rather than systemic process acute damage.

Language: Английский

Citations

0
Comparative analysis of metagenomic next-generation sequencing for pathogenic identification in clinical body fluid samples DOI Creative Commons
Ning Sun,

Jiaxun Zhang,

Wentao Guo

et al.

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: March 25, 2025

This study aims to evaluate and compare the effectiveness of metagenomic next-generation sequencing (mNGS) in identifying pathogens from clinical body fluid samples, with a specific focus on application microbial cell-free DNA (cfDNA) mNGS. A total 125 samples were collected. All underwent mNGS targeting whole-cell (wcDNA), 30 also analyzed for cfDNA 41 subjected 16S rRNA NGS comparative analysis. Patient data, including culture results, obtained electronic medical records. In comparison mNGS, mean proportion host wcDNA was 84%, significantly lower than 95% observed (p < 0.05). Using results as reference, concordance rates 63.33% (19/30) 46.67% (14/30) Additionally, showed greater consistency bacterial detection achieving rate 70.7% (29/41) compared 58.54% (24/41) NGS. The sensitivity specificity pathogen 74.07% 56.34%, respectively, when results. Whole-cell demonstrates higher identification both particularly those associated abdominal infections. However, compromised highlights necessity careful interpretation practice.

Language: Английский

Citations

0
The use of metagenomics to enhance diagnosis of Encephalitis DOI
Sarah Buddle,

Oscar Walter Torres,

Sofia Morfopoulou

et al.

Expert Review of Molecular Diagnostics, Journal Year: 2025, Volume and Issue: unknown

Published: May 7, 2025

Encephalitis has a broad etiology, including infectious and auto-immune causes. In encephalitis the breadth of causative organisms results in incomplete testing low diagnostic yields.Metagenomics sequences all DNA RNA allows untargeted detection single specimen; this is particular use diagnosis with etiology. We review literature discuss metagenomics workflows, host depletion pathogen enrichment methods, bioinformatics analysis potential transcriptome to aid diagnosis. clinical for neurological infection time result, cost, quality assurance, patient cohorts whom adds most value, recommended specimen types, limitations published cases which been used diagnose encephalitis. There good evidence utility Due infections rare, unexpected or novel pathogens, value immunocompromised patients greatest yield brain biopsies. Technical advances are needed reduce complexity, cost result will enable wider adoption laboratories as first line test.

Language: Английский

Citations

0
Active herpesviruses are associated with intensive care unit (ICU) admission in patients pulmonary infection and alter the respiratory microbiome DOI Creative Commons
Zhiguang Liu, Chunjian Qi, Yujia Shi

et al.

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 9, 2024

The Herpesviridae family contains several human-related viruses, which are able to establish colonizing and latency in the human body, posing a significant threat prognosis of patients. Pulmonary infections represent one predominant infectious diseases globally, characterized by diverse multifaceted clinical manifestations that have consistently attracted clinician's concern. However, relationship herpesviruses on pulmonary respiratory microbiota remains poorly understood.

Language: Английский

Citations

0