Unravelling staphylococcal small-colony variants in cardiac implantable electronic device infections: clinical characteristics, management, and genomic insights DOI Creative Commons
Si Liu, Hongbin Chen, Fangjie Xu

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 13

Published: Jan. 8, 2024

Staphylococcal small-colony variants (SCVs) are common in cardiac implantable electronic device (CIED) infections. This is the first retrospective and multi-case study on CIED infections due to staphylococcal SCVs, aiming provide a theoretical basis for clinical management of device-related caused by SCVs.

Language: Английский

Composition and liquid-to-solid maturation of protein aggregates contribute to bacterial dormancy development and recovery DOI Creative Commons
Celien Bollen, Sofie Louwagie,

Femke Deroover

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Jan. 26, 2025

Recalcitrant bacterial infections can be caused by various types of dormant bacteria, including persisters and viable but nonculturable (VBNC) cells. Despite their clinical importance, we know fairly little about dormancy development recovery. Previously, established a correlation between protein aggregation in Escherichia coli. Here, present further support for direct relationship both. Our experiments demonstrate that aggregates progressively sequester proteins involved energy production, thereby likely causing ATP depletion dormancy. Furthermore, structural features determine the cell's ability to exit resume growth. Proteins were shown first assemble liquid-like condensates solidify over time. This liquid-to-solid phase transition impedes aggregate dissolution, preventing growth resumption. data model which structure, rather than cellular activity, marks from persister VBNC state. are often authors explore how composition structure affect this

Language: Английский

Citations

3
Integration of proteome and metabolome profiling to reveal heat stress response and tolerance mechanisms of Serratia sp. AXJ-M for the bioremediation of papermaking black liquor DOI

Xuejiao An,

Ningjian Li,

Shulin Zhang

et al.

Journal of Hazardous Materials, Journal Year: 2023, Volume and Issue: 450, P. 131092 - 131092

Published: Feb. 26, 2023

Language: Английский

Citations

37
Macrophage internalization creates a multidrug-tolerant fungal persister reservoir and facilitates the emergence of drug resistance DOI Creative Commons
Amir Arastehfar, Farnaz Daneshnia,

Nathaly Cabrera

et al.

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: March 2, 2023

Abstract Candida glabrata is a major fungal pathogen notable for causing recalcitrant infections, rapid emergence of drug-resistant strains, and its ability to survive proliferate within macrophages. Resembling bacterial persisters, subset genetically drug-susceptible C. cells can lethal exposure the fungicidal echinocandin drugs. Herein, we show that macrophage internalization induces cidal drug tolerance in , expanding persister reservoir from which echinocandin-resistant mutants emerge. We this associated with non-proliferation triggered by macrophage-induced oxidative stress, deletion genes involved reactive oxygen species detoxification significantly increases mutants. Finally, amphotericin B kill intracellular reducing resistance. Our study supports hypothesis intra-macrophage recalcitrant/drug-resistant alternating strategies be developed eliminate reservoir.

Language: Английский

Citations

30
Environmental, mechanistic and evolutionary landscape of antibiotic persistence DOI
Celien Bollen, Elen Louwagie, Natalie Verstraeten

et al.

EMBO Reports, Journal Year: 2023, Volume and Issue: 24(8)

Published: July 3, 2023

Language: Английский

Citations

28
Cold atmospheric pressure plasma-antibiotic synergy in Pseudomonas aeruginosa biofilms is mediated via oxidative stress response DOI Creative Commons

Jordanne‐Amee Maybin,

Thomas P. Thompson, Padrig B. Flynn

et al.

Biofilm, Journal Year: 2023, Volume and Issue: 5, P. 100122 - 100122

Published: April 7, 2023

Cold atmospheric-pressure plasma (CAP) has emerged as a potential alternative or adjuvant to conventional antibiotics for the treatment of bacterial infections, including those caused by antibiotic-resistant pathogens. The sub-lethal CAP exposures synergise antimicrobials eradication

Language: Английский

Citations

26
Host stress drives tolerance and persistence: The bane of anti-microbial therapeutics DOI Creative Commons
Sophie Hélaine, Brian P. Conlon, Kimberly M. Davis

et al.

Cell Host & Microbe, Journal Year: 2024, Volume and Issue: 32(6), P. 852 - 862

Published: June 1, 2024

Antibiotic resistance, typically associated with genetic changes within a bacterial population, is frequent contributor to antibiotic treatment failures. persistence and tolerance, which we collectively term recalcitrance, represent transient phenotypic in the population that prolong survival presence of lethal concentrations antibiotics. recalcitrance challenging detect investigate-traditionally studied under vitro conditions, our understanding during infection its contribution failure limited. Recently, significant progress has been made study antibiotic-recalcitrant populations pathogenic species, including Mycobacterium tuberculosis, Staphylococcus aureus, Salmonella enterica, Yersiniae, context host environment. Despite diversity these pathogens models, shared signals responses promote common features vulnerabilities persisters tolerant bacteria have emerged. These will be discussed here, along toward developing therapeutic interventions better treat recalcitrant pathogens.

Language: Английский

Citations

13
A review of chemical signaling mechanisms underlying quorum sensing and its inhibition in Staphylococcus aureus DOI
Kayeen Vadakkan, Kuppusamy Sathishkumar, Sithara K. Urumbil

et al.

Bioorganic Chemistry, Journal Year: 2024, Volume and Issue: 148, P. 107465 - 107465

Published: May 16, 2024

Language: Английский

Citations

10
Piceatannol and its analogues alleviate Staphylococcus aureus pathogenesis by targeting β-lactamase biofilms and α-hemolysin DOI Creative Commons
Guizhen Wang, Jian Wen,

Zizeng Tian

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 14, 2025

Language: Английский

Citations

1
Understanding Staphylococcus aureus internalisation and induction of antimicrobial tolerance DOI Open Access
Frédéric Goormaghtigh, Françoise Van Bambeke

Expert Review of Anti-infective Therapy, Journal Year: 2024, Volume and Issue: 22(1-3), P. 87 - 101

Published: Jan. 5, 2024

, a human commensal, is also one of the most common and serious pathogens for humans. In recent years, its capacity to survive replicate in phagocytic non-phagocytic cells has been largely demonstrated. these intracellular niches, bacteria are shielded from immune response antibiotics, turning host into long-term infectious reservoirs. Moreover, neutrophils carry bloodstream, leading systemic spreading disease. Despite threat posed by

Language: Английский

Citations

7
Intracellular persister: A stealth agent recalcitrant to antibiotics DOI Creative Commons
Nicolas Personnic, Patricia Doublet, Sophie Jarraud

et al.

Frontiers in Cellular and Infection Microbiology, Journal Year: 2023, Volume and Issue: 13

Published: March 31, 2023

The bulk of bacteria transiently evading appropriate antibiotic regimes and recovered from non-resolutive infections are commonly refer to as persisters. In this mini-review, we discuss how persisters stem the interplay between pathogen cellular defenses mechanisms its underlying heterogeneity.

Language: Английский

Citations

14