Major alteration of lung microbiome and the host responses in critically ill COVID-19 patients with high viral load DOI Creative Commons
Ingrid G. Bustos, Rosana Wiscovitch-Russo, Harinder Singh

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 12, 2024

Patients with COVID-19 under invasive mechanical ventilation are at higher risk of developing ventilator-associated pneumonia (VAP), associated increased healthcare costs, and unfavorable prognosis. The underlying mechanisms this phenomenon have not been thoroughly dissected. Therefore, study attempted to bridge gap by performing a lung microbiota analysis evaluating the host immune responses that could drive development VAP. In prospective cohort study, mechanically ventilated patients confirmed SARS-CoV-2 infection were enrolled. Nasal swabs (NS), endotracheal aspirates (ETA), blood samples collected initially within 12 h intubation again 72 post-intubation. Plasma underwent cytokine metabolomic analyses, while NS ETA sequenced for microbiome examination. was categorized based on Data conducted using RStudio version 4.3.1. 36 ventilation, significant differences found in nasal pulmonary microbiome, notably Staphylococcus Enterobacteriaceae, linked VAP showed viral load respiratory samples, elevated neutralizing antibodies, reduced inflammatory cytokines, including IFN-δ, IL-1β, IL-12p70, IL-18, IL-6, TNF-α, CCL4. Metabolomic revealed changes 22 metabolites non-VAP 27 patients, highlighting D-Maltose-Lactose, Histidinyl-Glycine, various phosphatidylcholines, indicating metabolic predisposition This reveals critical link between alterations loads These findings provide novel insights into VAP, potential implications management prevention.

Language: Английский

Human nasal microbiota shifts in healthy and chronic respiratory disease conditions DOI Creative Commons

Aleksandras Konovalovas,

Julija Armalytė, Laurita Klimkaitė

et al.

BMC Microbiology, Journal Year: 2024, Volume and Issue: 24(1)

Published: April 27, 2024

Abstract Background An increasing number of studies investigate various human microbiotas and their roles in the development diseases, maintenance health states, balanced signaling towards brain. Current data demonstrate that nasal microbiota contains a unique highly variable array commensal bacteria opportunistic pathogens. However, we need to understand how harness current knowledge, enrich with beneficial microorganisms, prevent pathogenic developments. Results In this study, have obtained nasal, nasopharyngeal, bronchoalveolar lavage fluid samples from healthy volunteers patients suffering chronic respiratory tract diseases for full-length 16 S rRNA sequencing analysis using Oxford Nanopore Technologies. Demographic clinical were collected simultaneously. The microbiome 97 people Lithuania inflammatory disease revealed represents upper airways well. Conclusions was enriched pathogens, which could be used as indicators conditions. addition, observed contained several plant- bee-associated species, suggesting possibility enriching via such exposures when needed. These candidate probiotics should investigated modulating effects on airway lung epithelia, immunogenic properties, neurotransmitter content, maintaining nose-brain interrelationships.

Language: Английский

Citations

6
Salivary polyreactive antibodies andHaemophilus influenzaeare associated with respiratory infection severity in young children with recurrent respiratory infections DOI Creative Commons
Mischa H. Koenen, Wouter A. A. de Steenhuijsen Piters, Marien I. de Jonge

et al.

European Respiratory Journal, Journal Year: 2024, Volume and Issue: 64(4), P. 2400317 - 2400317

Published: Aug. 8, 2024

Background Recurrent respiratory tract infections (rRTIs) are a common reason for immunodiagnostic testing in children, which relies on serum antibody level measurements. However, because RTIs predominantly affect the mucosa, antibodies may inaccurately reflect local immune defences. We investigated responses saliva and their interplay with microbiota relation to RTI severity burden young children rRTIs. Methods conducted prospective cohort study including 100 aged <10 years rRTIs, family members healthy healthcare professionals. Total polyreactive concentrations were determined (ELISA); composition (16S rRNA sequencing) viruses (quantitative PCR) characterised nasopharyngeal swabs. Proteomic analysis (Olink) was performed samples. symptoms monitored daily mobile phone application assessed using latent class negative binomial mixed models. Results Serum levels not associated severity. Strikingly, 28% of salivary only 2% displayed polyreactivity (p<0.001). Salivary IgA negatively recurrent lower (adjusted OR 0.80, 95% CI 0.67–0.94) detection multiple 0.76, 0.61–0.96). Haemophilus influenzae abundance positively symptom (regression coefficient 0.05, 0.02–0.08). Conclusion These results highlight importance mucosal immunity burden, suggest that H. serve as indicators infection

Language: Английский

Citations

2
The Human Nasal Microbiome: A Perspective Study During the SARS-CoV-2 Pandemic in Malta DOI Creative Commons
David Pinzauti, Simon De Jaegher,

Maria D’Aguanno

et al.

Microorganisms, Journal Year: 2024, Volume and Issue: 12(12), P. 2570 - 2570

Published: Dec. 13, 2024

The human respiratory tract is colonized by a complex microbial community that helps maintain health and plays crucial role in defending the host from infections. Respiratory viruses have been demonstrated to alter microbiota composition, resulting opportunistic species expansion, increasing disease severity susceptibility bacterial co-infections. This study aims examine compositional differences nasal between SARS-CoV-2-infected non-infected patients. We conducted Oxford Nanopore full-length 16S rRNA sequencing on swabs 94 COVID-19 negative 85 positive patients collected during SARS-CoV-2 pandemic Malta. Our analysis identified significant alpha beta diversity composition among our groups. observed trend toward decreased richness evenness COVID-Positive cohort with increased abundance of common including Citrobacter koseri, Dolosigranulum pigrum, Haemophilus influenzae, Klebsiella pneumoniae, Moraxella catarrhalis. findings this are line previously published papers identifying key alterations associated infection. Understanding these microbiome-driven mechanisms could present novel prognostic markers or offer new approaches for prevention treatment.

Language: Английский

Citations

1
Major alteration of Lung Microbiome and the Host Reaction in critically ill COVID-19 Patients with high viral load DOI Creative Commons
Ingrid G. Bustos, Rosana Wiscovitch-Russo, Harinder Singh

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: March 8, 2024

Patients with COVID-19 under invasive mechanical ventilation are at higher risk of developing ventilator-associated pneumonia (VAP), associated increased healthcare costs, and unfavorable prognosis. The underlying mechanisms this phenomenon have not been thoroughly dissected. Therefore, study attempted to bridge gap by performing a lung microbiota analysis evaluating the host immune responses that could drive development VAP.

Language: Английский

Citations

0
Major alteration of lung microbiome and the host responses in critically ill COVID-19 patients with high viral load DOI Creative Commons
Ingrid G. Bustos, Rosana Wiscovitch-Russo, Harinder Singh

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 12, 2024

Patients with COVID-19 under invasive mechanical ventilation are at higher risk of developing ventilator-associated pneumonia (VAP), associated increased healthcare costs, and unfavorable prognosis. The underlying mechanisms this phenomenon have not been thoroughly dissected. Therefore, study attempted to bridge gap by performing a lung microbiota analysis evaluating the host immune responses that could drive development VAP. In prospective cohort study, mechanically ventilated patients confirmed SARS-CoV-2 infection were enrolled. Nasal swabs (NS), endotracheal aspirates (ETA), blood samples collected initially within 12 h intubation again 72 post-intubation. Plasma underwent cytokine metabolomic analyses, while NS ETA sequenced for microbiome examination. was categorized based on Data conducted using RStudio version 4.3.1. 36 ventilation, significant differences found in nasal pulmonary microbiome, notably Staphylococcus Enterobacteriaceae, linked VAP showed viral load respiratory samples, elevated neutralizing antibodies, reduced inflammatory cytokines, including IFN-δ, IL-1β, IL-12p70, IL-18, IL-6, TNF-α, CCL4. Metabolomic revealed changes 22 metabolites non-VAP 27 patients, highlighting D-Maltose-Lactose, Histidinyl-Glycine, various phosphatidylcholines, indicating metabolic predisposition This reveals critical link between alterations loads These findings provide novel insights into VAP, potential implications management prevention.

Language: Английский

Citations

0