COVID-19 vaccine antibody response is associated with side-effects, chronic health conditions, and vaccine type in a large Northern California cohort DOI Creative Commons
Olivia Solomon, Cameron Adams, Mary Horton

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2022, Volume and Issue: unknown

Published: Oct. 1, 2022

Abstract As vaccines have become available for COVID-19, it is important to understand factors that may impact response. The objective of this study describe vaccine response in a well-characterized Northern California cohort, including differences side-effects and antibody by type, sex, age, as well responses subjects with pre-existing health conditions are known risk more severe COVID-19 infection. From July 2020 March 2021, ∼5,500 adults from the East Bay Area were followed part longitudinal cohort study. Comprehensive questionnaire data biospecimens testing collected at multiple time-points. All least 18 years age members East-Bay who answered questionnaires related vaccination status two Three vaccines, Moderna (2 doses), Pfizer-BioNTech Johnson & (single dose), examined exposures. Additionally, assessed. main outcomes interest anti-SARS-CoV-2 Spike (measured S/C ratio Ortho VITROS assay) self-reporting 11 potential side effects. When comparing both doses respective Pfizer-BioNTech, participants receiving had higher odds many reported side-effects. same was true single-dose dose 2 vaccine. also increased each additional side-effect after second dose. ratios lower aged 65 older, females. At all timepoints, recipients ratio. Individuals fully vaccinated 72.4% compared those Moderna. Subjects asthma, diabetes, cardiovascular disease demonstrated than 20% decrease In support previous findings, we show We observed associated side-effects, history responses. This information consider further recommended.

Language: Английский

Rapid quantitation of SARS-CoV-2 antibodies in clinical samples with an electrochemical sensor DOI Open Access
Sharma T. Sanjay, Nolan Durr, Pawan Jolly

et al.

Biosensors and Bioelectronics, Journal Year: 2022, Volume and Issue: 223, P. 115037 - 115037

Published: Dec. 24, 2022

Language: Английский

Citations

18
­­Evaluating blood sampling strategies within the SIREN study: the experience from a large cohort of healthcare workers in the UK DOI Creative Commons
Nipunadi Hettiarachchi,

Debbie Blick,

Tom Coleman

et al.

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

Abstract Background Delivering research studies that require a large number of samples to monitor specific populations is complex, often resulting in high costs and intricate logistics. We aim describe the processes for blood sample collection management evaluate alternative sampling methods within cohort healthcare workers UK (the SIREN study). Methods conducted process evaluation. First, we described across different study periods from June 2020 March 2024 how these evolved over time. Secondly, compared sampling: venous phlebotomy (hospital-based) vs. capillary (at-home). Results The main challenges with stemmed scale use decentralised 135 hospital sites during COVID-19 pandemic. adapted our as progressed, overcoming most challenges. When comparing hospital-based at-home sampling, overall, return rates taken at home were higher than site- based (80% vs 71%, respectively). At-home took less time be returned UKHSA Laboratory testing (median 2 days; interquartile (IQ) 2-3) 6 IQ 3-8). However, more likely considered void (4%) when tested (0%). Cost was almost 3-times (£34.05 £11.50, respectively), however larger volumes obtained via (8 ml 600 µl whole blood). Conclusions Sample are complex. Our results support an effective cheaper strategy therefore should method future research. Trial registration number: ISRCTN11041050 - date 12/01/2021.

Language: Английский

Citations

0
Quantifying neutralising antibody responses against SARS-CoV-2 in dried blood spots (DBS) and paired sera DOI Creative Commons
Kelly J. Roper,

Jordan Thomas,

Wejdan Albalawi

et al.

Scientific Reports, Journal Year: 2023, Volume and Issue: 13(1)

Published: Sept. 11, 2023

Abstract The ongoing SARS-CoV-2 pandemic was initially managed by non-pharmaceutical interventions such as diagnostic testing, isolation of positive cases, physical distancing and lockdowns. advent vaccines has provided crucial protection against SARS-CoV-2. Neutralising antibody (nAb) responses are a key correlate protection, therefore measuring nAb is essential for monitoring vaccine efficacy. Fingerstick dried blood spots (DBS) ideal use in large-scale sero-surveillance because they inexpensive, offer the option self-collection can be transported stored at ambient temperatures. Such advantages also make DBS appealing to resource-limited settings potential future pandemics. In this study, sera, venous fingerstick on filter paper were measured. Samples collected from acutely infected individuals, convalescent individuals vaccinated individuals. Good agreement observed between measured eluted paired sera. Stability sera room temperature 28 days. Overall, study provides support viable sample collection method responses.

Language: Английский

Citations

6
Remote HbA1c testing via microsampling: fit for purpose? DOI Open Access
Nick Verougstraete, Veronique Stove, Christophe P. Stove

et al.

Clinical Chemistry and Laboratory Medicine (CCLM), Journal Year: 2023, Volume and Issue: 62(1), P. 3 - 17

Published: July 8, 2023

Abstract The collection of capillary blood microsamples via finger-prick has several advantages over traditional collection. It is considered convenient and more patient-centric, enabling the sample by patient at her/his home with subsequent analysis in lab following postal shipment. Determination diabetes biomarker HbA 1c self-collected to remotely monitor patients seems be a very promising option which could eventually lead better treatment adaptations disease control. This especially convenient/relevant for living areas where venipuncture impractical, or support virtual consultations using telemedicine. Over years, substantial numbers reports on microsampling have been published. However, heterogeneity applied study designs data evaluation remarkable. review provides general critical overview these papers, along specific points attention that should dealt when aiming implementing reliable determination. We focus used (dried) techniques, conditions, stability microsamples, extraction, analytical methods, method validation, correlation studies conventional venous samples satisfaction. Lastly, possibility liquid instead dried discussed. Liquid expected similar as suggest it suitable approach collect lab.

Language: Английский

Citations

5
At-home sampling to meet geographical challenges for serological assessment of SARS-CoV-2 exposure in a rural region of northern Sweden, March to May 2021: a retrospective cohort study DOI Creative Commons

Julia Wigren Byström,

Linnea Vikström,

Ebba Rosendal

et al.

Eurosurveillance, Journal Year: 2023, Volume and Issue: 28(13)

Published: March 30, 2023

BackgroundThe current SARS-CoV-2 pandemic has highlighted a need for easy and safe blood sampling in combination with accurate serological methodology. Venipuncture testing is usually performed by trained staff at healthcare centres. Long travel distances to centres rural regions may introduce bias of towards relatively large communities closer access. Rural are therefore often not represented population-based data.AimThe aim this retrospective cohort study was develop implement strategy at-home region Sweden during spring 2021, evaluate its role provide equal health care inhabitants.MethodsWe developed sensitive method measure antibodies the S-protein optimised assay clinical use together capillary sampling.ResultsWe demonstrated that our ELISA gave comparable results after analysis or serum from SARS-CoV-2-experienced individuals. We stability under conditions reflected temperature humidity winter summer. By assessment samples 4,122 individuals, we could show both feasibility implementation shifted geographical spread favour areas.ConclusionImplementation enabled citizens living remote areas access centralised laboratory antibody tests. The used here enable disease control authorities get rapid information concerning immunity infectious diseases, even across vast distance.

Language: Английский

Citations

5
Health inequities in SARS-CoV-2 infection, seroprevalence, and COVID-19 vaccination: Results from the East Bay COVID-19 study DOI Creative Commons
Cameron Adams, Mary Horton, Olivia Solomon

et al.

PLOS Global Public Health, Journal Year: 2022, Volume and Issue: 2(8), P. e0000647 - e0000647

Published: Aug. 15, 2022

Comprehensive data on transmission mitigation behaviors and both SARS-CoV-2 infection serostatus are needed from large, community-based cohorts to identify COVID-19 risk factors the impact of public health measures. We conducted a longitudinal, population-based study in East Bay Area Northern California. From July 2020-March 2021, approximately 5,500 adults were recruited followed over three collection rounds investigate association between geographic demographic characteristics behavior with prevalence. estimated populated-adjusted prevalence antibodies vaccination, self-reported test positivity. Population-adjusted seroprevalence was low, increasing 1.03% (95% CI: 0.50–1.96) Round 1 (July-September 2020), 1.37% 0.75–2.39) 2 (October-December 2.18% 1.48–3.17) 3 (February-March 2021). vaccination 21.64% 19.20–24.34) 3, White individuals having 4.35% 0.35–8.32) higher vaccine than identifying as African American or Black, Indian Alaskan Native, Asian, Hispanic, two more races, other. No evidence for an observed. Despite >99% participants reporting wearing masks other, well those lower-income households, lower-educated had highest lowest seroprevalence. Results demonstrate that effective policies address these disparities inequities.

Language: Английский

Citations

7
West African pre-pandemic cross-reactive antibody and cellular responses against SARS-CoV-2 DOI Creative Commons
Bobby Brooke Herrera, Beth Chaplin, Souleymane Mboup

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: May 22, 2024

Abstract The COVID-19 pandemic had a severe impact globally, yet African populations exhibited unexpectedly lower rates of disease and mortality. We investigated the potential role pre-existing immunity in shaping epidemiology Africa. Leveraging paired plasma peripheral blood mononuclear cells collected from Senegalese female sex workers prior to pandemic, we observed substantial levels cross-reactive SARS-CoV-2, stemming exposure seasonal human coronaviruses (hCoVs). Our antibody analysis revealed 23.5% (47/200) seroprevalence rate against SARS-CoV-2 nucleocapsid (N). Of these N-reactives, 85.1% (40/47), 44.7% (21/47), 95.7% (45/47) showed reactivity hCoV-229E or hCoV-OC43 spike (S) and/or N hCoV-HKU1 S. cellular responses also demonstrated cross-reactivity with 82.2% (37/45) 84.4% (38/45) showing IFN-γ S N, respectively. These findings suggest that hCoV may induce adaptive immunity, potentially contributing protection COVID-19. A unique pre-pandemic subject antibodies detectable neutralization responses. study provides insights into dynamics West underscores importance understanding outcomes globally.

Language: Английский

Citations

1
Is the stability of folates in dried blood microsamples sufficient to perform home-sampling studies? DOI Creative Commons
Liesl Heughebaert, Christophe P. Stove

The Analyst, Journal Year: 2023, Volume and Issue: 149(3), P. 895 - 908

Published: Dec. 26, 2023

Dried blood microsampling is increasingly used for home-sampling and epidemiological studies because of its multiple advantages, including an often greatly improved analyte stability. However, a critical assessment the stability under realistic conditions should always be performed as part validation, especially unstable molecules like folates (vitamin B9). Here, objective was to determine whether folate in dried microsamples sufficient allow set-up monitoring status

Language: Английский

Citations

2
Organophosphate Pesticide Exposure And Risk Of Sars-Cov-2 Infection DOI
Luis López,

Katherine Kogut,

Stephen Rauch

et al.

Published: Jan. 1, 2024

Download This Paper Open PDF in Browser Add to My Library Share: Permalink Using these links will ensure access this page indefinitely Copy URL DOI

Language: Английский

Citations

0
Quantitating SARS-CoV-2 Neutralizing Antibodies from Human Dried Blood Spots DOI Creative Commons

Katherine Berman,

Greta Van Slyke,

Hayley Novak

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 20, 2024

ABSTRACT Background In the earliest days of COVID-19 pandemic, collection dried blood spots (DBS) enabled public health laboratories to undertake population-scale seroprevalence studies estimate rates SARS-CoV-2 exposure. With seropositivity levels now estimated exceed 94% in United States, attention has turned using DBS assess functional (neutralizing) antibodies within cohorts interest. Methods Contrived eluates from convalescent, fully vaccinated and pre-COVID-19 serum samples were evaluated plaque reduction neutralization titer (PRNT) assays, a specific 8-plex microsphere immunoassay, cell-based pseudovirus assay, two different spike-ACE2 inhibition an in-house Luminex-based RBD-ACE2 assay commercial real-time PCR-based (NAB-Sure™). Results convalescent individuals compatible with but not assays or PRNT. However, insensitivity was overcome high antibody titers. Conclusion neutralizing titers can be derived confidence eluates, thereby opening door use these biospecimens for analysis vulnerable populations normally hard reach communities.

Language: Английский

Citations

0