medRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2022,
Volume and Issue:
unknown
Published: Oct. 1, 2022
Abstract
As
vaccines
have
become
available
for
COVID-19,
it
is
important
to
understand
factors
that
may
impact
response.
The
objective
of
this
study
describe
vaccine
response
in
a
well-characterized
Northern
California
cohort,
including
differences
side-effects
and
antibody
by
type,
sex,
age,
as
well
responses
subjects
with
pre-existing
health
conditions
are
known
risk
more
severe
COVID-19
infection.
From
July
2020
March
2021,
∼5,500
adults
from
the
East
Bay
Area
were
followed
part
longitudinal
cohort
study.
Comprehensive
questionnaire
data
biospecimens
testing
collected
at
multiple
time-points.
All
least
18
years
age
members
East-Bay
who
answered
questionnaires
related
vaccination
status
two
Three
vaccines,
Moderna
(2
doses),
Pfizer-BioNTech
Johnson
&
(single
dose),
examined
exposures.
Additionally,
assessed.
main
outcomes
interest
anti-SARS-CoV-2
Spike
(measured
S/C
ratio
Ortho
VITROS
assay)
self-reporting
11
potential
side
effects.
When
comparing
both
doses
respective
Pfizer-BioNTech,
participants
receiving
had
higher
odds
many
reported
side-effects.
same
was
true
single-dose
dose
2
vaccine.
also
increased
each
additional
side-effect
after
second
dose.
ratios
lower
aged
65
older,
females.
At
all
timepoints,
recipients
ratio.
Individuals
fully
vaccinated
72.4%
compared
those
Moderna.
Subjects
asthma,
diabetes,
cardiovascular
disease
demonstrated
than
20%
decrease
In
support
previous
findings,
we
show
We
observed
associated
side-effects,
history
responses.
This
information
consider
further
recommended.
Environmental Research,
Journal Year:
2024,
Volume and Issue:
255, P. 119214 - 119214
Published: May 23, 2024
Several
studies
have
reported
immune
modulation
by
organophosphate
(OP)
pesticides,
but
the
relationship
between
OP
exposure
and
SARS-CoV-2
infection
is
yet
to
be
studied.
We
used
two
different
measures
of
pesticide
(urinary
biomarkers
(N
=
154)
residential
proximity
applications
292))
examine
association
early-childhood
lifetime
OPs
risk
using
antibody
data.
Our
study
population
consisted
young
adults
(ages
18-21
years)
from
Center
for
Health
Assessment
Mothers
Children
Salinas
(CHAMACOS)
Study,
a
longitudinal
cohort
families
California
agricultural
region.
Urinary
reflected
in
utero
age
5
years.
Residential
exposures
16
antibodies
blood
samples
collected
June
2022
January
2023
were
detected
via
enzyme
linked
immunosorbent
assays,
each
designed
bind
antigens.
performed
logistic
regression
measure
exposure,
adjusting
covariates
demographic
data
self-reported
questionnaire
found
increased
odds
among
participants
with
higher
urinary
(OR
1.94,
95%
CI:
0.71,
5,58)
0-5
1.90,
0.54,
6.95).
PLOS Global Public Health,
Journal Year:
2024,
Volume and Issue:
4(6), P. e0003320 - e0003320
Published: June 14, 2024
Testing
for
human
immunodeficiency
virus
(HIV)
and
hepatitis
C
(HCV)
using
dried
blood
spot
(DBS)
specimens
has
been
an
integral
part
of
bio-behavioural
surveillance
in
Canada
almost
two
decades,
though
less
is
known
regarding
the
use
DBS
other
sexually
transmitted
blood-borne
infections
(STBBI).
A
systematic
review
was
conducted
a
peer-reviewed
search
strategy
to
assess
current
evidence
validity
STBBI
testing
specimens.
Eligibility
criteria
included
studies
reporting
with
either
commercially
available
or
"in-house"
tests
populations
15
years
age
older.
Studies
measure
such
as
sensitivity,
specificity,
positive
negative
predictive
values
were
eligible
inclusion.
Quality
risk
bias
assessed
QUADAS-2
tool.
total
7,132
records
identified.
Of
these,
174
met
Among
that
reported
measures,
substantial
proportion
demonstrated
high
sensitivity
(≥90%)
62.5%
cases
(N
=
334/534
measurements),
specificity
observed
84.9%
instances
383/451
measurements).
However,
quality
varied
greatly.
Our
findings
support
where
sufficient
available,
but
highly
dependent
on
thorough
method
development
validation.
Indian Journal of Medical Microbiology,
Journal Year:
2024,
Volume and Issue:
51, P. 100700 - 100700
Published: Aug. 13, 2024
COVID-19
disease
continues
to
be
a
global
health
concern.
The
current
protocol
for
detecting
SARS-CoV-2
requires
healthcare
professionals
draw
blood
from
patients.
Recent
studies
showed
that
dried
spot
(DBS)
is
valuable
sampling
procedure
can
collect
low
volume
without
the
need
presence
of
medical
practitioners.
This
study
synthesized
available
literature
on
using
DBS
as
collection
tool
diagnose
disease.
Microbiology Spectrum,
Journal Year:
2024,
Volume and Issue:
unknown
Published: Oct. 29, 2024
ABSTRACT
In
the
earliest
days
of
COVID-19
pandemic,
collection
dried
blood
spots
(DBS)
enabled
public
health
laboratories
to
undertake
population-scale
seroprevalence
studies
used
estimate
rates
SARS-CoV-2
exposure.
With
seropositivity
levels
now
estimated
exceed
94%
in
United
States,
attention
has
turned
using
DBS
assess
neutralizing
antibodies
within
cohorts
interest.
this
goal
mind,
we
generated
contrived
(cDBS)
and
whole
blood-derived
from
convalescent
vaccinated
individuals
subjected
eluates
a
battery
assays,
including
multiplexed
microsphere
immunoassay
(MIA),
receptor
binding
domain
(RBD)-human
ACE2
inhibition
assay
(iACE2),
cell-based
pseudovirus
neutralization
assay,
real-time
PCR-based
surrogate
(NAB-Sure).
The
results
were
benchmarked
against
paired
serum
samples
tested
clinically
validated
plaque
reduction
titer
(PRNT)
assay.
an
8-plex
MIA
NAB-Sure
assays
demonstrated
highly
significant
correlations
with
PRNT
values
when
evaluated
panel
86
serum–DBS
samples.
Both
are
adaptable
automated
liquid
handlers
for
high-throughput
capacity.
While
limited
ancestral
WA1,
study
nonetheless
represents
important
proof
concept
demonstrating
potential
utility
as
biospecimen
type
use
assessing
immunity
at
community
population
levels.
IMPORTANCE
variants
concern
continue
circulate
globally
remain
serious
threat
large
segments
population.
From
standpoint,
identifying
vulnerable
communities
based
on
immune
status
is
critical
terms
vaccine
booster
recommendations.
report,
investigated
which
antibody
titers.
Using
DBS,
demonstrate
that
readily
measurable
correlate
Moreover,
several
methods
platforms.
medRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: April 7, 2023
Abstract
Objective
Testing
for
human
immunodeficiency
virus
(HIV)
and
hepatitis
C
(HCV)
using
dried
blood
spot
(DBS)
specimens
has
been
an
integral
part
of
bio-behavioural
surveillance
in
Canada
almost
two
decades.
A
systematic
review
was
conducted
to
assess
the
current
evidence
regarding
validity
sexually
transmitted
blood-borne
infection
(STBBI)
testing
DBS
specimens.
Methods
literature
search
a
peer-reviewed
strategy.
Eligibility
criteria
included
studies
reporting
use
STBBI
populations
15
years
age
or
older.
The
intervention
interest
either
commercially
available
“in-house”
tests
used
detect
from
Studies
that
reported
measure
such
as
sensitivity,
specificity,
positive
negative
predictive
values
were
eligible
inclusion.
Quality
risk
bias
assessed
QUADAS-2
tool.
Results
total
6,706
records
identified.
Of
these
records,
169
full-text
articles
met
with
most
on
HIV
(
n
=73),
followed
by
HCV
=63),
HBV
=33),
syphilis
=7),
HAV
=5),
HSV
HTLV
=3),
HPV
=1).
majority
high
sensitivity
(≥90%)
specificity
(≥90%).
However,
quality
varied
greatly.
No
found
chlamydia
gonorrhoea
Conclusion
Our
findings
support
where
sufficient
available,
but
is
highly
dependent
thorough
method
development
validation.
medRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2022,
Volume and Issue:
unknown
Published: Oct. 1, 2022
Abstract
As
vaccines
have
become
available
for
COVID-19,
it
is
important
to
understand
factors
that
may
impact
response.
The
objective
of
this
study
describe
vaccine
response
in
a
well-characterized
Northern
California
cohort,
including
differences
side-effects
and
antibody
by
type,
sex,
age,
as
well
responses
subjects
with
pre-existing
health
conditions
are
known
risk
more
severe
COVID-19
infection.
From
July
2020
March
2021,
∼5,500
adults
from
the
East
Bay
Area
were
followed
part
longitudinal
cohort
study.
Comprehensive
questionnaire
data
biospecimens
testing
collected
at
multiple
time-points.
All
least
18
years
age
members
East-Bay
who
answered
questionnaires
related
vaccination
status
two
Three
vaccines,
Moderna
(2
doses),
Pfizer-BioNTech
Johnson
&
(single
dose),
examined
exposures.
Additionally,
assessed.
main
outcomes
interest
anti-SARS-CoV-2
Spike
(measured
S/C
ratio
Ortho
VITROS
assay)
self-reporting
11
potential
side
effects.
When
comparing
both
doses
respective
Pfizer-BioNTech,
participants
receiving
had
higher
odds
many
reported
side-effects.
same
was
true
single-dose
dose
2
vaccine.
also
increased
each
additional
side-effect
after
second
dose.
ratios
lower
aged
65
older,
females.
At
all
timepoints,
recipients
ratio.
Individuals
fully
vaccinated
72.4%
compared
those
Moderna.
Subjects
asthma,
diabetes,
cardiovascular
disease
demonstrated
than
20%
decrease
In
support
previous
findings,
we
show
We
observed
associated
side-effects,
history
responses.
This
information
consider
further
recommended.
