Geobacter sulfurreducens electroactive biofilms on Fe2O3/FTO support-electrodes for developing a sodium acetate electrochemical biosensor DOI Creative Commons
G.A. Huerta-Miranda, Luis M. Rodríguez-Torres, A. Martinez-García

et al.

Biosensors and Bioelectronics X, Journal Year: 2023, Volume and Issue: 14, P. 100370 - 100370

Published: June 16, 2023

Geobacter sulfurreducens is an essential microorganism in biogeochemical cycles. It used biotechnologically bioremediation and electricity production. The importance of G. lies its capacity to contact extracellular electron acceptors, including several electrode materials. Taking advantage the versatility grow on different surfaces, it possible design synthesize new electrodes that can expand improve applications these bacteria. In this work, we performed multidisciplinary research studies demonstrate innovative application well-known microbial system. interaction between fluorine-doped tin oxide (FTO) ordinary glass with was enhanced by modifying surface Fe2O3 films. As confocal laser microscopy analyses revealed, enhancement promoted biofilm development, whose parameters were significantly better than bare support-electrodes. Furthermore, provided evidence interacted dissolved film during incubation. Then, when added sodium acetate system, metabolism activated decreased electrochemical response compound. We benefited from behavior develop a voltammetric-based robust biosensor could measure at high concentrations acceptable linear interval, 10 110 mM, LOD = 5.9 ± mM LOQ 18.1 1.5 mM.

Language: Английский

Bidirectional extracellular electron transfer pathways of Geobacter sulfurreducens biofilms: Molecular insights into extracellular polymeric substances DOI
Guiqin Yang, Xue Xia,

Weijie Nie

et al.

Environmental Research, Journal Year: 2023, Volume and Issue: 245, P. 118038 - 118038

Published: Dec. 24, 2023

Language: Английский

Citations

12
Decoupled respiration in electro-active bacteria DOI Creative Commons
Diego A. Massazza, Juan Pablo Busalmen, Hernán E. Romeo

et al.

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: May 2, 2025

Studies on electron-transfer pathways in certain bacterial strains have revealed that the degree of coupling electron transfer to proton translocation along respiratory chain can be regulated according metabolic demands. This first line response, based existence energy dissipation mechanisms, has not been demonstrated a general pattern across kingdom, let alone operative electro-active bacteria. In this study, we hypothesized cells should respond over-polarization by also triggering decoupling mechanisms prevent overloads. Based electrochemical analyses, propose recently discovered inner-membrane cytochrome CbcBA - used Geobacter sulfurreducens bacteria for cellular respiration near thermodynamic energetic limit act as an gate when metabolism is demanded, contributing regulate balance cell carbon assimilation from electrode respiration.

Language: Английский

Citations

0
Intracytoplasmic membranes develop in Geobacter sulfurreducens under thermodynamically limiting conditions DOI Creative Commons
Ethan Howley, Anna Mangus, Dewight Williams

et al.

npj Biofilms and Microbiomes, Journal Year: 2023, Volume and Issue: 9(1)

Published: April 7, 2023

Abstract Geobacter sulfurreducens is an electroactive bacterium capable of reducing metal oxides in the environment and electrodes engineered systems 1,2 . sp are keystone organisms electrogenic biofilms, as their respiration consumes fermentation products produced by other reduces a terminal electron acceptor e.g. iron oxide or electrode. To respire extracellular acceptors with wide range redox potentials, G. has complex network respiratory proteins, many which membrane-bound 3–5 We have identified intracytoplasmic membrane (ICM) structures This ICM invagination inner that folded organized unknown mechanism, often but not always located near tip cell. Using confocal microscopy, we can identify at least half cells contain when grown on low potential anode surfaces, whereas higher surfaces using fumarate had significantly lower frequency. 3D models developed from cryo-electron tomograms show to be continuous extension contact cytoplasmic periplasmic space. The differential abundance under different thermodynamic conditions supports hypothesis it adaptation limited energy availability, increase proteins could flux. Thus, provides extra inner-membrane surface these proteins. first Thermodesulfobacterium metal-oxide reducer found produce ICMs.

Language: Английский

Citations

7
Comparing theoretical and practical biomass yields calls for revisiting thermodynamic growth models for electroactive microorganisms DOI
Benjamin Korth, João Pereira, Tom Sleutels

et al.

Water Research, Journal Year: 2023, Volume and Issue: 242, P. 120279 - 120279

Published: June 29, 2023

Language: Английский

Citations

7
Oxygen In The Mix: Is Oxic Microbial Electrosynthesis A Potential Alternative For Biomass Production? DOI Creative Commons
Johannes Eberhard Reiner, Benjamin Korth, Miriam Edel

et al.

ChemElectroChem, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 6, 2024

Abstract Oxic microbial electrosynthesis (oMES) allows the utilization of renewable electricity and industrial gas streams containing CO 2 O for biomass production by cultivating aerobic, autotrophic, hydrogen‐oxidizing bacteria, commonly known as Knallgas bacteria. oMES is likely not a direct competitor to conventional anoxic harnessing aerobic bacteria depends on energetically inefficient assimilatory reduction pathways. However, it might be complementary approach classical from perspective limited land use availability cheap energy. The best characterized bacterium Cupriavidus necator . Extensively studied lithoautotrophic host, C. already offers broad arsenal genetic tools. In contrast, mechanistical knowledge about recently discovered Kyrpidia spormannii limited, but this species shows remarkable growth when cultivated cathodic biofilm in bioelectrochemical systems. addition, first experiments indicate low energy demand production, which order magnitude fermentation with or heterotrophic methanotrophic technologies. Still, many aspects electrochemical cultivation K. need better understood rigorously improved competitive technology making, including electron transfer kinetics, conditions, mass balances, reactor design.

Language: Английский

Citations

2
Starvation combined with constant anode potential triggers intracellular electron storage in electro-active biofilms DOI Creative Commons
João Pereira, Patricia Regina Neves, Vivian Nemanic

et al.

Water Research, Journal Year: 2023, Volume and Issue: 242, P. 120278 - 120278

Published: June 27, 2023

The accumulation of electrons in the form Extracellular Polymeric Substances (EPS) and poly-hydroxyalkanoates (PHA) has been studied anaerobic processes by adjusting access microorganisms to electron donor final acceptor. In Bio-electrochemical systems (BESs), intermittent anode potential regimes have also recently used study storage anodic electro-active biofilms (EABfs), but effect feeding mode on not explored. Therefore, this study, EPS PHA was as a function operating conditions. EABfs were grown under both constant fed with acetate (electron donor) continuously or batch. Confocal Laser Scanning Microscopy (CLSM) Fourier-Transform Infrared Spectroscopy (FTIR) assess storage. range Coulombic efficiencies, from 25 82%, biomass yields, between 10 20%, indicate that could an alternative consuming process. From image processing, 0.92 pixel ratio poly-hydroxybutyrate (PHB) amount cells found batch EABf potential. This linked presence living Geobacter shows energy gain carbon source starvation triggers for intracellular highest content (extracellular storage) observed potential, showing acceptor leads formation excess gained. Tailoring conditions can thus steer microbial community result trained perform desired biological conversion, which be beneficial more efficient optimized BES.

Language: Английский

Citations

3
Geobacter sulfurreducens electroactive biofilms on Fe2O3/FTO support-electrodes for developing a sodium acetate electrochemical biosensor DOI Creative Commons
G.A. Huerta-Miranda, Luis M. Rodríguez-Torres, A. Martinez-García

et al.

Biosensors and Bioelectronics X, Journal Year: 2023, Volume and Issue: 14, P. 100370 - 100370

Published: June 16, 2023

Geobacter sulfurreducens is an essential microorganism in biogeochemical cycles. It used biotechnologically bioremediation and electricity production. The importance of G. lies its capacity to contact extracellular electron acceptors, including several electrode materials. Taking advantage the versatility grow on different surfaces, it possible design synthesize new electrodes that can expand improve applications these bacteria. In this work, we performed multidisciplinary research studies demonstrate innovative application well-known microbial system. interaction between fluorine-doped tin oxide (FTO) ordinary glass with was enhanced by modifying surface Fe2O3 films. As confocal laser microscopy analyses revealed, enhancement promoted biofilm development, whose parameters were significantly better than bare support-electrodes. Furthermore, provided evidence interacted dissolved film during incubation. Then, when added sodium acetate system, metabolism activated decreased electrochemical response compound. We benefited from behavior develop a voltammetric-based robust biosensor could measure at high concentrations acceptable linear interval, 10 110 mM, LOD = 5.9 ± mM LOQ 18.1 1.5 mM.

Language: Английский

Citations

2