A Novel Effector FoUpe9 Enhances the Virulence of Fusarium oxysporum f. sp. cubense Tropical Race 4 by Inhibiting Plant Immunity DOI Creative Commons
Zheng Cong,

Yini Ma,

Lisha Zeng

et al.

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(4), P. 308 - 308

Published: April 13, 2025

Fusarium wilt caused by oxysporum f. sp. cubense tropical race 4 (Foc TR4) is the most destructive disease of banana. Effectors play a crucial role in Foc TR4–banana interaction; however, only few effectors have been functionally characterized. Our previous secretome studies on TR4 highlighted an uncharacterized protein without any conserved domains (named FoUpe9), which was predicted to be candidate effector. Herein, bioinformatics analysis showed that FoUpe9 highly among species. induced during early infection stages A yeast signal sequence trap assay secretory protein. could inhibit cell death and ROS accumulation triggered BAX through Agrobacterium-mediated Nicotiana benthamiana expression system. Subcellular location located nucleus cytoplasm N. cells. Deletion gene did not affect mycelial growth, conidiation, sensitivity cell-wall integrity, or osmotic oxidative stress, but significantly attenuated fungal virulence. deletion diminished colonization production SA-related defense genes banana plants. These results suggest enhances virulence inhibiting host immune responses provide new insights into functions proteins, further enhancing our understanding effector-mediated pathogenesis.

Language: Английский

A Novel Effector FoUpe9 Enhances the Virulence of Fusarium oxysporum f. sp. cubense Tropical Race 4 by Inhibiting Plant Immunity DOI Creative Commons
Zheng Cong,

Yini Ma,

Lisha Zeng

et al.

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(4), P. 308 - 308

Published: April 13, 2025

Fusarium wilt caused by oxysporum f. sp. cubense tropical race 4 (Foc TR4) is the most destructive disease of banana. Effectors play a crucial role in Foc TR4–banana interaction; however, only few effectors have been functionally characterized. Our previous secretome studies on TR4 highlighted an uncharacterized protein without any conserved domains (named FoUpe9), which was predicted to be candidate effector. Herein, bioinformatics analysis showed that FoUpe9 highly among species. induced during early infection stages A yeast signal sequence trap assay secretory protein. could inhibit cell death and ROS accumulation triggered BAX through Agrobacterium-mediated Nicotiana benthamiana expression system. Subcellular location located nucleus cytoplasm N. cells. Deletion gene did not affect mycelial growth, conidiation, sensitivity cell-wall integrity, or osmotic oxidative stress, but significantly attenuated fungal virulence. deletion diminished colonization production SA-related defense genes banana plants. These results suggest enhances virulence inhibiting host immune responses provide new insights into functions proteins, further enhancing our understanding effector-mediated pathogenesis.

Language: Английский

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