KlebsiellaPneumoniae turns more virulent under flow stresses in capillary like microchannels DOI Creative Commons
Siddhant Jain, Anmol Singh, Nivedita Tiwari

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 18, 2023

Abstract Fluidic habitats are very common to bacterial life, however, little is known about the effect of flow stresses on virulence bacteria. In present work, we conduct microfluidic experiments understand consequence generated by flowing fluid morphology and virulence. We consider Klebsiella pneumoniae ( KP ), an ESKAPE pathogen as model bacteria that responsible for blood stream infections like bacteremia apart from pneumonia, urinary tract more. generate four different stress conditions changing rate channel geometry subsequently altering shear stressing time (τ). observe significant changes in structural aspects stressed With increase parameters, viability sample deteriorated. Most importantly, these samples proliferate much more than unstressed inside RAW264.7 murine macrophages. The results shed light complex relationship between Furthermore, challenge with ciprofloxacin see how they behave under conditions. study can be extended deadly diseases using organ-on-a-chip technology help pathogenicity realistic environments. Figure: A schematic representation work. Figure created BioRender www.biorender.com )

Language: Английский

Airborne survival and stress response in Listeria monocytogenes across different growth temperatures DOI

So-Seum Yong,

Jae-Ik Lee,

Dong‐Hyun Kang

et al.

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 468, P. 133706 - 133706

Published: Feb. 9, 2024

Language: Английский

Citations

1
Adenosine Monophosphate as a Metabolic Adjuvant Enhances Antibiotic Efficacy against Drug-Resistant Bacterial Pathogens DOI Creative Commons

Wenxuan Zhang,

Zhen‐Yi Wu,

Zulifukeer Maituersong

et al.

Pharmaceuticals, Journal Year: 2024, Volume and Issue: 17(7), P. 933 - 933

Published: July 11, 2024

Global bacterial infections are on the rise, and drug resistance to bacteria is gradually rendering existing antibiotics ineffective. Therefore, discovery of new strategies urgently needed. Cellular metabolism a key factor in regulation resistance, which cannot be separated from utilization energetic substances, suggesting that substances may associated with resistance. In this study, we found adenosine monophosphate (AMP) can enhance bactericidal effect gentamicin against gentamicin-resistant Staphylococcus aureus. This synergistic generalized for use different Gram-positive or Gram-negative bacteria. We also validated mechanism AMP reversal antibiotic involves enhancing proton motive force via tricarboxylic acid cycle increase uptake. Simultaneously, increases oxidative stress-induced cell death. study presents strategy adopting low-dose control drug-resistant bacteria, important future development control.

Language: Английский

Citations

1
Stability of influenza A virus in droplets and aerosols is heightened by the presence of commensal respiratory bacteria DOI Creative Commons
Shannon C. David, Aline Schaub,

Céline Terrettaz

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Feb. 5, 2024

Abstract Aerosol transmission remains a major challenge for the control of respiratory viruses, particularly those that cause recurrent epidemics, like influenza A virus (IAV). These viruses are rarely expelled alone, but instead embedded in consortium microorganisms populate tract. The impact microbial communities and inter-pathogen interactions upon stability transmitted is well-characterised pathogens gut, under-studied niche. Here, we assessed whether presence 5 different species common commensal bacteria could influence IAV within droplets deposited on surfaces airborne aerosol particles at typical indoor air humidity. It was found bacterial stationary droplets, either mixed community or individual strains, resulted 10- to 100-fold more infectious remaining after 1 hour. Bacterial viability not required this viral stabilisation, though maintained morphology seemed be essential. Additionally, non-respiratory tested here had little stabilising effect, indicating phenomenon respiratory-specific. protective stabilised via induction early efflorescence due flattened droplet during drying. Even when no occurred high humidity bacteria-induced changes were abolished by aerosolization deposition well-plate, remained protective. This indicates an additional stabilisation mechanism currently unknown. Notably, equivalent density offered varying degrees protection with Gram-positive Staphylococcus aureus Streptococcus pneumoniae being most robustly stabilising. suggests composition individual’s microbiota previously un-considered host-specific factor influencing efficacy spread. Identifying novel factors such as can environment will further increase our understanding risks, provide opportunities limit spread infections populations. Synopsis Our findings have significant environmental health relevance, they identify host potentially contributing environments.

Language: Английский

Citations

1
Ambient Carbon Dioxide Concentration Correlates with SARS-CoV-2 Aerostability and Infection Risk DOI Creative Commons
Allen E. Haddrell, Henry P. Oswin, Mara Otero-Fernandez

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 10, 2023

Abstract An improved understanding of the underlying physicochemical properties respiratory aerosol that influence viral infectivity may open new avenues to mitigate transmission diseases such as COVID-19. Previous studies have shown a rapid increase in pH aerosols following generation due changes gas-particle partitioning buffering bicarbonate ions and carbon dioxide is significant factor reducing infectivity. We show here aerostability results from moderate atmospheric concentration (e.g. 1,800 ppm), an effect more marked than observed for relative humidity. model likelihood COVID-19 on ambient CO2, concluding even CO2 overall risk. These observations confirm critical importance ventilation maintaining low concentrations indoor environments mitigating disease transmission. Moreover, impact correlating with suggests increased risks pathogen will accompany our climate changes.

Language: Английский

Citations

3
Recovery and Survival of Aerosolized Escherichia coli and Enterococcus faecium on Food‐Grade Rubber, HDPE Plastic, Stainless Steel, and Waxed Cardboard DOI Creative Commons
Tuan Anh Le, Joseph D. Eifert,

Cyril A. Etaka

et al.

Journal of Food Safety, Journal Year: 2024, Volume and Issue: 44(6)

Published: Dec. 1, 2024

ABSTRACT Contamination of food contact surfaces by airborne transmission pathogens from the environment has contributed to disease outbreaks. Therefore, this study evaluated survival and recovery aerosolized generic Escherichia coli Enterococcus faecium four (food‐grade silicone rubber, high‐density polyethylene [HDPE] plastic, stainless steel, waxed cardboard), after times (10, 20, 40, 60 min), two relative humidity (RH) levels (high: 80%–90%, low: 40%–50%), three distances aerosolization source (0, 36.5, 73 cm; E. only), with without airflow ( only). ANOVA test Tukey's HSD at α = 0.05 was used determine how treatment combinations influenced recovery. At high humidity, on all materials 40 min ~1.0 log lower than 10 min, further reduced 1.0 min. 20 compared humidity. Distances exerted no significance, whereas presence lowered declined ~5.0 CFU/coupon 0 h 3.5 6 h, 2.0 24 post‐inoculation. significantly p < 0.05) cardboard. Low RH longer time but not . consistent across combinations, changes 0.5 CFU/coupon. The findings are relevant for bacteria common potential products.

Language: Английский

Citations

0
Dependence of the Concentrations of Culturable Microorganisms and Total Protein on Meteorological Conditions in the Novosibirsk Surroundings DOI
А. С. Сафатов,

Н. А. Лаптева,

S. E. Olkin

et al.

Atmospheric and Oceanic Optics, Journal Year: 2024, Volume and Issue: 37(5), P. 637 - 643

Published: Oct. 1, 2024

Language: Английский

Citations

0
Observations on phenomenological changes in Klebsiella Pneumoniae under fluidic stresses DOI
Siddhant Jain, Anmol Singh, Nivedita Tiwari

et al.

Soft Matter, Journal Year: 2023, Volume and Issue: 19(47), P. 9239 - 9253

Published: Jan. 1, 2023

In the present work, experiments are conducted to understand consequence of stresses generated by flowing fluid on bacterial morphology and virulence in microfluidic channels.

Language: Английский

Citations

1
KlebsiellaPneumoniae turns more virulent under flow stresses in capillary like microchannels DOI Creative Commons
Siddhant Jain, Anmol Singh, Nivedita Tiwari

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 18, 2023

Abstract Fluidic habitats are very common to bacterial life, however, little is known about the effect of flow stresses on virulence bacteria. In present work, we conduct microfluidic experiments understand consequence generated by flowing fluid morphology and virulence. We consider Klebsiella pneumoniae ( KP ), an ESKAPE pathogen as model bacteria that responsible for blood stream infections like bacteremia apart from pneumonia, urinary tract more. generate four different stress conditions changing rate channel geometry subsequently altering shear stressing time (τ). observe significant changes in structural aspects stressed With increase parameters, viability sample deteriorated. Most importantly, these samples proliferate much more than unstressed inside RAW264.7 murine macrophages. The results shed light complex relationship between Furthermore, challenge with ciprofloxacin see how they behave under conditions. study can be extended deadly diseases using organ-on-a-chip technology help pathogenicity realistic environments. Figure: A schematic representation work. Figure created BioRender www.biorender.com )

Language: Английский

Citations

0