The Role of the Vaginal and Endometrial Microbiomes in Infertility and Their Impact on Pregnancy Outcomes in Light of Recent Literature

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 13227 - 13227

Published: Dec. 9, 2024

The Human Microbiome Project (HMP), initiated in 2007, aimed to gather comprehensive knowledge create a genetic and metabolic map of human-associated microorganisms their contribution physiological states predisposition certain diseases. Research has revealed that the human microbiome is highly diverse exhibits significant interpersonal variability; consequently, its exact impact on health remains unclear. With development next-generation sequencing (NGS) technologies, broad spectrum microbial communities been better characterized. lower female genital tract, particularly vagina, colonized by various bacterial species, with

Language: Английский

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Changes in cytokine and sequestosome-1 levels during twin pregnancy progression: Association with outcome

Cytokine, Journal Year: 2024, Volume and Issue: 180, P. 156668 - 156668

Published: June 7, 2024

Twin pregnancies are associated with complications and adverse outcomes. The number of twin has increased in the last decades, due to use assisted reproductive techniques delayed childbearing. Analysis changes that occur during pregnancy progression their association outcome will lead improved clinical interventions.

Language: Английский

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Exploring the Vaginal Microbiome During Pregnancy: Microbial Diversity, E. coli pathogenicity, and Links to Urinary Tract Colonization

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 28, 2024

This study explores the role of vaginal microbiota (VM) in pathophysiology asymptomatic bacteriuria (ASB), which requires systematic screening and antibiotic treatment during pregnancy. We hypothesize that disruptions VM composition may promote ascending colonization bacteria from gut to urinary tract, reinforcing existence a gut-urogenital axis. A healthy is typically characterized by low diversity dominated lactic acid-producing species, notably those Lactobacillus genus. Diversity analyses whole genomes metagenomic sequencing data 1,553 pregnant women revealed signature an increase phylogenetic within ASB. shift includes both decrease lactobacilli abundance taxa associated with microbiota, particularly Enterobacterales, substantial rise Escherichia coli abundance. Worldwide, E. remains most common etiological agent pregnancy also major causative newborn infections. Given its importance limited on characterization pregnancy, we performed genomic assembly analysis this species cohort. Molecular typing antimicrobial resistance 72 assembled Extra-Intestinal pathogenic (“ExPEC”) strains, are involved various extraintestinal infections such as tract infections, bacteremia. Moreover, presence environment was variation microbial diversity, primarily marked species. Overall, our shows how disruption key bacterial group can disrupt stability, potentially leading opportunistic pathogens.

Language: Английский

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Group B Streptococcus vaginal colonisation throughout pregnancy is associated with decreased Lactobacillus crispatus and increased Lactobacillus iners abundance in the vaginal microbial community

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: Sept. 30, 2024

Group B Streptococcus (GBS) asymptomatically colonises the vagina of up to 40% pregnant women and can transmit neonates during birth, causing neonatal pneumonia, sepsis, meningitis, significant mortality. Vaginal GBS colonisation be attributed a range host bacterial factors, which may include composition vaginal microbial community. There are few studies that have examined community in relation throughout pregnancy. Here, we performed 16S rRNA sequencing (V3-V4) on swabs from at 24- 36-weeks’ gestation, who were culture-negative or culture-positive either 24 weeks 36 weeks’ gestation both timepoints. 93 analysed; 46 culture-negative, 11 only, 21 only 15 timepoints Brilliance agar. V3-V4 gene amplicon demonstrated G. vaginalis was significantly more abundant 24-weeks’ despite lack changes richness between 36-week samples. The communities persistently colonised with GBS, had higher abundance Lactobacillus iners , compared other groups where L. crispatus, gasseri jensenii dominant. We characterised pregnancy status, longitudinal study for first time. most interesting finding pregnancy, there increase reduction crispatus abundance. Given detail role plays literature, it is imperative relationship this unique environmental niche further investigated.

Language: Английский

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Vaginal Microbiome and the Risk of Preterm Birth in Women Living With HIV: A Scoping Review

American Journal of Reproductive Immunology, Journal Year: 2024, Volume and Issue: 92(5)

Published: Oct. 30, 2024

There are sparse data on the role of vaginal microbiome (VMB) in pregnancy among pregnant women living with HIV (PWLWH) and its association spontaneous preterm birth (sPTB). We conducted a scoping review to assess associations between microbiota sPTB PWLWH. Three studies were included, representing total 180 PWLWH out 652 pregnancies. All used modern DNA sequencing methods (16S rRNA amplification, metagenomics, or metatranscriptomics). had higher VMB richness diversity compared HIV-uninfected rates two three studies. A proportion was observed those Lactobacillus-deficient, anaerobe-dominant microbiota. In studies, concentrations inflammation markers associated increased diversity. status independently sPTB. It is unclear if microbial contributes more PTB, but does appear alter individuals may also affect PTB microbiome-independent pathways. Given limited number heterogeneity sample size, collection methods, inconsistent results it difficult causally link HIV, VMB, inflammatory cytokines,

Language: Английский

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The microbiome in pregnancy and early life—Highlights from the 11th Maria Delivoria‐Papadopoulos Perinatal Symposium

Acta Paediatrica, Journal Year: 2024, Volume and Issue: unknown

Published: June 19, 2024

Abstract This review was based on a symposium that examined novel aspects of the microbiome during pregnancy and early life explored papers published by lecturers. For example, it showed bacterial extracellular vesicles derived from harboured in various maternal niches, carried deoxyribonucleic acid, were isolated placenta may have confounded placental studies. Maternal diet responsible for composition diversity breast milk microbiota, shaped offspring's influenced their immune components. Probiotics antibiotics administered perinatally had beneficial but also long‐lasting adverse effects offspring.

Language: Английский

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