Investigation of Nitrogen Conversion Efficiency in Hydrogen-based Autotrophic Nitrate Reduction Reactor

Research Square (Research Square), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 10, 2025

This study investigates the performance of a hydrogen-based membrane biofilm reactor (MBfR) for nitrogen transformation over an extended operational period exceeding 200 days. During monitoring, sustained highly alkaline conditions with pH consistently 11.20, while achieving ammonia conversion efficiency above 60% and maintaining nitrite accumulation rate below 1%. Integrated mass balance calculations microbial community profiling revealed coexistence denitrification dissimilatory nitrate reduction to ammonium (DNRA) pathways within network. A factorial experimental design was implemented hydraulic retention time (HRT) influent concentration as independent variables, generating nine distinct regimes through cross-variable permutations. Subsequent analysis extensive datasets enabled development Response Surface Methodology (RSM) model simulate dynamics. Model validation confirmed statistical reliability predictive accuracy RSM framework. Critical demonstrated that in hydrogen autotrophic system exhibited significant correlation individual variables themselves but showed negligible dependence on their interactive effects. These findings provide novel insights into process optimization strategies theoretical understanding convention.

Language: Английский

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Investigation of nitrogen conversion efficiency in hydrogen-based autotrophic nitrate reduction reactor

Journal of Water Process Engineering, Journal Year: 2025, Volume and Issue: 72, P. 107536 - 107536

Published: March 22, 2025

Language: Английский

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Novel thermophilic genera Geochorda gen. nov. and Carboxydochorda gen. nov. from the deep terrestrial subsurface reveal the ecophysiological diversity in the class Limnochordia

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Sept. 23, 2024

The class Limnochordia harbors a single cultivated member, the mesophilic Limnochorda pilosa , which was isolated from meromictic lake. Despite numerous molecular signatures reported in various ecosystems, ecophysiological versatility of this deeply branched lineage Firmicutes (Bacillota) remains poorly understood. objective study to use targeted cultivation, based on metagenome-assembled genomes deep terrestrial aquifer Western Siberia, isolate two new thermophilic members class. These isolates, described as Geochorda subterranea gen. nov. sp. and Carboxydochorda within Geochordaceae fam. nov., were capable both anaerobic aerobic respiration using fumarate O 2 respectively, with simple sugars electron donors. have demonstrated fermentative growth degradation polymers, including starch, maltose, maltodextrin, xylan, chitin. carboxydotrophic C. exhibited autotrophic via Calvin–Benson–Bassham cycle, CO, H formate donors an acceptor, adding metabolic flexibility bacterium nutrient-depleted “deep biosphere” supporting possibility metabolism subsurface. broad physiological potential deciphered experiments comparative genomic data explains widespread distribution uncultivated where they can oxidize complex organic substrates through respiration, well pursue chemolithotrophic lifestyle oxidation or CO.

Language: Английский

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Alkalothermophilic Iron-Reducing Bacteria of the Goryachinsk Thermal Water Basin

Microbiology, Journal Year: 2024, Volume and Issue: 93(6), P. 864 - 875

Published: Nov. 29, 2024

Abstract A study of iron-reducing prokaryotes that use the poorly crystalline mineral ferrihydrite as an electron acceptor revealed their widespread occurrence on planet. However, ability iron reducers to develop under polyextreme conditions (at elevated values pH and temperature) has not been previously demonstrated. To confirm existence alkalothermophilic reducers, we studied water sediment samples from Goryachinsk nitrogen-rich thermal basin. These springs are associated with young tectonic fractures in Baikal rift zone; is characterized by low salinity, alkaline (8.8‒9.2) temperature 53‒55°C. Enrichment cultures reducing synthesized were obtained adding this springs, followed anaerobic incubation at 50 55°C. The 16S rRNA gene profiling primary enrichment high taxonomic diversity, almost exclusively represented bacterial taxa. Subsequent transfers organic acids or hydrogen donors resulted stable organo- lithotrophic reducers. Members genus Parvivirga , one first cultured representatives OPB41 group (now order Anaerosomatales phylum Actinomycetota ), constituted 30 50% total abundance.

Language: Английский

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Alkalo-thermophilic iron-reducing bacteria of the Goryachinskoe thermal water deposit

Микробиология, Journal Year: 2024, Volume and Issue: 93(6), P. 818 - 831

Published: Dec. 15, 2024

Language: Английский

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Low-Abundance Sulfidogenic Bacteria Carry out Intensive Sulfate Reduction in Terrestrial Hydrotherms of the Barguzin Valley

Microbiology, Journal Year: 2023, Volume and Issue: 92(S1), P. S29 - S32

Published: Dec. 1, 2023

Language: Английский

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