Response to: ‘No long-term effect of past Pneumocystis jirovecii pneumonia on pulmonary function in people with HIV’

AIDS, Journal Year: 2023, Volume and Issue: 37(14), P. 2267 - 2268

Published: Oct. 25, 2023

aDivision of Pulmonary, Critical Care, Allergy and Sleep Medicine bDivision HIV, Infectious Diseases Global Medicine, University California San Francisco, California, USA. Correspondence to Katerina L. Byanova, MD, MS, Division 505 Parnassus Ave, Box 0111, CA 94143, E-mail: [email protected] Received 21 July, 2023 Accepted 9 August,

Language: Английский

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Pulmonary Function in People Living With Human Immunodeficiency Virus: A Meta-Analysis

Archivos de Bronconeumología, Journal Year: 2024, Volume and Issue: 60(4), P. 200 - 206

Published: Jan. 19, 2024

Language: Английский

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Novel Approach to Exploring Protease Activity and Targets in Hiv-Associated Obstructive Lung Disease Using Combined Proteomic-Peptidomic Analysis

Published: Jan. 1, 2024

Background: Obstructive lung disease (OLD) is increasingly prevalent among persons living with HIV (PLWH). However, the role of proteases in HIV-associated OLD remains unclear.Methods: We combined proteomics and peptidomics to comprehensively characterize protease activities. mass spectrometry (MS) analysis on bronchoalveolar lavage fluid (BALF) peptides proteins from PLWH (n=25) without (n=26) a targeted Somascan aptamer-based proteomic approach quantify individual assess their correlation function. Endogenous mapped native identify substrates activity. Using MEROPS database, we identified candidate linked peptide generation based binding site affinities which were assessed via z-scores. used t-tests compare average forced expiratory volume 1 second per predicted value (FEV1pp) between samples detection each cleaved protein adjusted for multiple comparisons by controlling false discovery rate (FDR).Findings: 101 proteases, 95 had functional network associations 22 correlated FEV1pp. These included cathepsins, metalloproteinases (MMP), caspases neutrophil elastase. discovered 31 subject proteolytic cleavage that associate FEV1pp, top pathways involved small ubiquitin-like modifier mediated modification (SUMOylation). Proteases elastase, granzyme, cathepsin D.Interpretations: In OLD, significant number are up-regulated, many degradation. degrade cell cycle stability, thereby disrupting critical biological functions.Funding: Supported National Institutes Health grant R01 HL140971-01A1 (all authors). This material also result work supported resources use facilities at Minneapolis Veterans Affairs Medical Center, Minneapolis, USA.Declaration Interest: I have no competing interests declare. Ethical Approval: Participants parent cohort studies provided informed consent BALF collection storage approval respective Institutional Review Boards Pittsburgh Vancouver. The current study was approved University Minnesota Board.

Language: Английский

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Novel Approach to Exploring Protease Activity and Targets in HIV-associated Obstructive Lung Disease using Combined Proteomic-Peptidomic Analysis

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: June 4, 2024

Background: Obstructive lung disease (OLD) is increasingly prevalent among persons living with HIV (PLWH). However, the role of proteases in HIV-associated OLD remains unclear. Methods: We combined proteomics and peptidomics to comprehensively characterize protease activities. mass spectrometry (MS) analysis on bronchoalveolar lavage fluid (BALF) peptides proteins from PLWH (n=25) without (n=26) a targeted Somascan aptamer-based proteomic approach quantify individual assess their correlation function. Endogenous mapped native identify substrates activity. Using MEROPS database, we identified candidate linked peptide generation based binding site affinities which were assessed via z-scores. used t-tests compare average forced expiratory volume 1 second per predicted value (FEV1pp) between samples detection each cleaved protein adjusted for multiple comparisons by controlling false discovery rate (FDR). Findings: 101 proteases, 95 had functional network associations 22 correlated FEV1pp. These included cathepsins, metalloproteinases (MMP), caspases neutrophil elastase. discovered 31 subject proteolytic cleavage that associate FEV1pp, top pathways involved small ubiquitin-like modifier mediated modification (SUMOylation). Proteases elastase, granzyme, cathepsin D. Interpretations: In OLD, significant number are up-regulated, many degradation. degrade cell cycle stability, thereby disrupting critical biological functions.

Language: Английский

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Pulmonary comorbidities in people with HIV− the microbiome connection

Current Opinion in HIV and AIDS, Journal Year: 2024, Volume and Issue: 19(5), P. 246 - 252

Published: June 25, 2024

Purpose of review To report recent evidence on associations between human microbiome, particularly airway and gut, pulmonary comorbidities in people with HIV (PWH). Furthermore, we explore how changes the microbiome may contribute to immune dysregulation higher rates among PWH. Finally, propose future directions field. Recent findings Increased risk rapid lung function decline have been reported even well treated Altered microbiota profiles PWH as compared those without. The most consistent data association HIV-related comorbidities, oral dysbiosis, which has also associated distinct respiratory mucosal inflammatory short-term mortality. However, a possible causal link remains be elucidated. Summary Associations HIV-associated studies. Yet underlying mechanism underpinning observed is largely unknown substantial knowledge gaps remain. Future research warranted unveil role from different anatomical compartments relation

Language: Английский

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Novel approach to exploring protease activity and targets in HIV-associated obstructive lung disease using combined proteomic-peptidomic analysis

Respiratory Research, Journal Year: 2024, Volume and Issue: 25(1)

Published: Sept. 10, 2024

Language: Английский

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Do people living with HIV have accelerated lung function decline, despite adequate treatment?

Thorax, Journal Year: 2023, Volume and Issue: 78(6), P. 529 - 530

Published: Feb. 20, 2023

Language: Английский

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