A novel immobilization enzymatic system of nanocellulose-based β-glucosidase in O-glycoside bioconversion of phytochemicals

Cellulose, Journal Year: 2025, Volume and Issue: unknown

Published: March 10, 2025

Language: Английский

Characterization of a Metallic-Ions-Independent L-Arabinose Isomerase from Endophytic Bacillus amyloliquefaciens for Production of D-Tagatose as a Functional Sweetener

Fermentation, Journal Year: 2023, Volume and Issue: 9(8), P. 749 - 749

Published: Aug. 12, 2023

D-Tagatose is a low-calorie sugar substitute that has gained increased attention as functional sweetener owing to its nutraceutical and prebiotic properties. Traditionally, D-tagatose produced via the enzymatic conversion of L-galactose by L-arabinose isomerase (L-AI). Nonetheless, most reported L-AI enzymes are ion-dependent requiring Mn2+ and/or Co2+ cofactors for their reactions, which limits application due safety health concerns. Herein, we addressed facile bioconversion using novel recombinant metallic-ions-independent derived from endophytic Bacillus amyloliquefaciens CAAI isolated cantaloupe fruits. The ORF (1500 bp) gene (araA) was cloned over-expressed in Escherichia coli. enzyme (BAAI) purified homogeneity Ni-NTA affinity chromatography, yielding single distinct band with an apparent molecular mass approximately 59 kDa deduced SDS-PAGE analysis. showed optimum activity at pH temperature 7.5 45 °C, respectively, obvious presence ethylenediaminetetraacetic acid (EDTA), indicating metallic-ions independence BAAI. Km values BAAI D-galactose were 251.6 mM 92.8 mM, respectively. catalytic efficiency (kcat/Km) found be 2.34 46.85 mM–1 min–1, results revealed production 47.2 g/L (100 g/L) 47.2% metallic-ions-free reaction system could implemented safe-production food-grade sweetener, D-tagatose.

Language: Английский

Bioprospecting of Culturable Halophilic Bacteria Isolated from Mediterranean Solar Saltern for Extracellular Halotolerant Enzymes

Microbiology and Biotechnology Letters, Journal Year: 2024, Volume and Issue: 52(1), P. 76 - 87

Published: March 15, 2024

Language: Английский