Nature Protocols, Journal Year: 2024, Volume and Issue: 19(4), P. 1122 - 1148
Published: Jan. 23, 2024
Language: Английский
Citations
9Current Landscape of Advanced Imaging Tools for Pathology Diagnostics
Modern Pathology, Journal Year: 2024, Volume and Issue: 37(4), P. 100443 - 100443
Published: Feb. 3, 2024
Language: Английский
Citations
5Towards next-generation diagnostic pathology: AI-empowered label-free multiphoton microscopy
Light Science & Applications, Journal Year: 2024, Volume and Issue: 13(1)
Published: Sept. 14, 2024
Language: Английский
Citations
43D imaging and pathological analysis of microglia in LPS-treated mice with light-sheet fluorescence microscopy
Journal of Neuroimmunology, Journal Year: 2025, Volume and Issue: 399, P. 578525 - 578525
Published: Jan. 16, 2025
Language: Английский
Citations
0First application of three‐dimensional light sheet fluorescence microscopy to human testicular tumors: New perspectives in histopathology
Andrology, Journal Year: 2025, Volume and Issue: unknown
Published: Feb. 12, 2025
Abstract Background Testicular tumors are among the most frequently diagnosed cancers in young men. The consequences of this diagnosis orchiectomies, severely restricting fertility. For these patients, a comprehensive diagnostics would be desirable, achieving refined and improved therapeutic patient stratification. Objective aim study was to use three‐dimensional (3D) light sheet fluorescence microscopy (LSFM) analyze complete testicular tumor punch at subcellular resolution—allowing detailed diagnostic assessment entire punch. Materials methods Tissue punches (3 5 mm diameter) were taken from paraffin blocks four miscellaneous tumors. After deparaffinization clearing using benzoic acid/benzyl benzoate, label‐free LSFM autofluorescence imaging performed. In addition, TO‐PRO‐3 nuclear stain applied several punches. scan, samples embedded again physically sectioned for conventional planar histology. Results Based on specific autofluorescence, not only general morphology tissue identified datasets, but also features like infiltrations, papillary pagetoid cell formations, germ neoplasia situ azoospermia. Subcellular characteristics such as vacuolated cytoplasm pleomorphic nuclei could detected maximum magnification. staining, virtual H&E sections reconstructed data tomographically visualized across Subsequent histology immunohistochemistry after analyses is possible. Discussion analysis enables 2D/3D an relevant due its intrinsic or with staining. Conclusion provides technical basis biopsies, thus maximizing spatial morphological anatomical information. 3D has potential identify new cancer biomarkers that have additional prognostic value patients.
Language: Английский
Citations
0An end-to-end workflow for non-destructive 3D pathology
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown
Published: Aug. 6, 2023
Recent advances in 3D pathology offer the ability to image orders-of-magnitude more tissue than conventional while providing a volumetric context that is lacking with 2D sections, all without requiring destructive sectioning. Generating high-quality datasets on consistent basis non-trivial, careful attention many details regarding preparation, imaging, and data/image processing an iterative process. Here we provide end-to-end protocol covering aspects of workflow (using light-sheet microscopy as illustrative imaging platform) sufficient detail perform well-controlled preclinical clinical studies. While compatible diverse staining protocols computationally generated color palettes for visual analysis, this will focus fluorescent analog hematoxylin eosin (H&E), which remains most common stain gold-standard diagnostic determinations. We present our guidelines broad range end-users (e.g., biologists, researchers, engineers) simple tutorial format.
Language: Английский
Citations
7Engineering the future of
The Journal of Pathology Clinical Research, Journal Year: 2023, Volume and Issue: 10(1)
Published: Nov. 2, 2023
Abstract In recent years, technological advances in tissue preparation, high‐throughput volumetric microscopy, and computational infrastructure have enabled rapid developments nondestructive 3D pathology, which high‐resolution histologic datasets are obtained from thick specimens, such as whole biopsies, without the need for physical sectioning onto glass slides. While pathology generates massive that attractive automated analysis, there is also a desire to use improve visual assessment of histology. this perspective, we discuss provide examples potential advantages clinical specimens challenges dealing with large (of individual or multiple specimens) pathologists not been trained interpret. We artificial intelligence triaging algorithms explainable analysis methods assist other domain experts interpretation these novel, often complex, datasets.
Language: Английский
Citations
6Increased Multiplexity in Optical Tissue Clearing-Based Three-Dimensional Immunofluorescence Microscopy of the Tumor Microenvironment by Light-Emitting Diode Photobleaching
Laboratory Investigation, Journal Year: 2024, Volume and Issue: 104(6), P. 102072 - 102072
Published: April 27, 2024
Language: Английский
Citations
2X-ray phase-contrast 3D virtual histology characterises complex tissue architecture in colorectal cancer
Frontiers in Gastroenterology, Journal Year: 2023, Volume and Issue: 2
Published: Oct. 24, 2023
Precise morphological analysis of tumour tissue samples is crucial for accurate diagnosis and staging colorectal cancer (CRC), but remains limited by the 2D nature conventional histology. Our aim to offer a 3D representation means X-ray-based imaging facilitate evaluation clinically relevant features in tissue, process that currently subject various restrictions. In this study, we show propagation-based synchrotron radiation-based free propagation phase-contrast microcomputed tomography (SRµCT) suitable generation volumes with 2-µm voxel size using standard formalin-fixed, paraffin-embedded from CRC patients provides sufficient contrast virtual We demonstrate that, an existing registration pipeline, histologic haematoxylin–eosin slice can be placed context µCT volume. The precisely registered section then used as “seed point” segmentation depiction major features. This approach allows more comprehensive understanding organisation space respect other structures such vessels, fat, lymph nodes, has potential improve patients’ prognostic outcomes.
Language: Английский
Citations
6Adaptations of membrane trafficking in cancer and tumorigenesis
Journal of Cell Science, Journal Year: 2024, Volume and Issue: 137(10)
Published: May 15, 2024
ABSTRACT Membrane trafficking, a fundamental cellular process encompassing the transport of molecules to specific organelles, endocytosis at plasma membrane and protein secretion, is crucial for homeostasis signalling. Cancer cells adapt trafficking enhance their survival metabolism, understanding these adaptations vital improving patient responses therapy identifying therapeutic targets. In this Review, we provide concise overview major pathways detail in pathways, including COPII-dependent endoplasmic reticulum (ER)-to-Golgi vesicle COPI-dependent retrograde Golgi-to-ER endocytosis, that have been found cancer. We explore how confer growth advantages or resistance cell death conclude by discussing potential utilising knowledge developing new treatment strategies overcoming drug cancer patients.
Language: Английский
Citations
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