Kinetic Features of Degradation of R-Loops by RNase H1 from Escherichia coli DOI Open Access
А. А. Кузнецова,

I. A. Kosarev,

Nadezhda A. Timofeyeva

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 12263 - 12263

Published: Nov. 15, 2024

R-loops can act as replication fork barriers, creating transcription-replication collisions and inducing stress by arresting DNA synthesis, thereby possibly causing aberrant processing the formation of strand breaks. RNase H1 (RH1) is one enzymes that participates in R-loop degradation cleaving RNA within a hybrid RNA-DNA duplex. In this study, kinetic features interaction RH1 from

Language: Английский

Transcription: friend or foe of genome stability DOI
Emmanuel Compe, Donata Orioli

FEBS Letters, Journal Year: 2025, Volume and Issue: 599(2), P. 143 - 146

Published: Jan. 1, 2025

Language: Английский

Citations

0
Mechanisms underlining R-loop biology and implications for human disease DOI Creative Commons
Junzhe Liu,

Fengze Li,

Yulong Cao

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: Feb. 21, 2025

R-loops are three-stranded non-canonical nucleic acid structures composed of nascent RNA hybridized with the template DNA strand, leaving non-template strand displaced. These play crucial roles in regulating gene expression, replication, and transcription processes. However, have also been increasingly described as highly deleterious, causing genomic instability damage. To maintain at a relatively safe level, complex regulatory mechanisms exist to prevent their excessive formation. The growing understanding R-loop functions has provided valuable insights into structure potential clinical applications. Emerging research indicates that contribute pathogenesis various disorders, including neurodegenerative, immune-related, neoplastic diseases. This review summarizes metabolism its significance etiology associated disorders. By elucidating governing R-loops, we aim establish theoretical foundation for disease exploring novel therapeutic strategies targeting these hybrid structures.

Language: Английский

Citations

0
Epitranscriptome in action: RNA modifications in the DNA damage response DOI Creative Commons
Blerta Xhemalçe, Kyle M. Miller, Natalia Gromak

et al.

Molecular Cell, Journal Year: 2024, Volume and Issue: 84(19), P. 3610 - 3626

Published: Oct. 1, 2024

Language: Английский

Citations

3
Cross-regulation of RNA methylation modifications and R-loops: from molecular mechanisms to clinical implications DOI Creative Commons
Yuqing Wu, Lin Shen, Hong Chen

et al.

Cellular and Molecular Life Sciences, Journal Year: 2024, Volume and Issue: 82(1)

Published: Dec. 10, 2024

R-loops, RNA–DNA hybrid structures, are integral to key cellular processes such as transcriptional regulation, DNA replication, and repair. However, aberrant accumulation of R-loops can compromise genomic integrity, leading the development various diseases. Emerging evidence underscores pivotal role RNA methylation modifications, particularly N6-methyladenosine (m6A) 5-methylcytosine (m5C), in orchestrating formation, resolution, stabilization R-loops. These modifications dynamically regulate R-loop metabolism, exerting bidirectional control by either facilitating or resolving structures during gene expression regulation damage Dysregulation resultant imbalance homeostasis closely linked pathogenesis diseases cancer neurodegenerative disorders. Thus, deciphering cross-talk between is essential for understanding mechanisms underlying stability identifying novel therapeutic targets. This review provides a comprehensive analysis dynamics, examines their physiological pathological implications, proposes future directions intervention targeting these processes.

Language: Английский

Citations

2
RNA Polymerase II is a Polar Roadblock to a Progressing DNA Fork DOI Creative Commons
Teresa Kay, James T. Inman,

Lucyna Lubkowska

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 13, 2024

DNA replication and transcription occur simultaneously on the same template, leading to inevitable conflicts between replisome RNA polymerase. These can stall fork threaten genome stability. Although numerous studies show that head-on are more detrimental prone promoting R-loop formation than co-directional conflicts, fundamental cause for polymerase roadblock polarity remains unclear, structure of these R-loops is speculative. In this work, we use a simple model system address complex question by examining Pol II advanced via mechanical unzipping mimic progression. We found binds stably resist removal in configuration, even with minimal transcript size, demonstrating has an inherent polarity. However, elongating long becomes potent persistent while retaining polarity, RNA-DNA hybrid mediates enhancement. Surprisingly, discovered when collides backtracked, form lagging strand front II, creating topological lock traps at fork. TFIIS facilitates severing connection hybrid. further demonstrate prime T7 still bound DNA. Our findings capture basal properties interactions fork, revealing significant implications transcription-replication conflicts.

Language: Английский

Citations

1
Kinetic Features of Degradation of R-Loops by RNase H1 from Escherichia coli DOI Open Access
А. А. Кузнецова,

I. A. Kosarev,

Nadezhda A. Timofeyeva

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(22), P. 12263 - 12263

Published: Nov. 15, 2024

R-loops can act as replication fork barriers, creating transcription-replication collisions and inducing stress by arresting DNA synthesis, thereby possibly causing aberrant processing the formation of strand breaks. RNase H1 (RH1) is one enzymes that participates in R-loop degradation cleaving RNA within a hybrid RNA-DNA duplex. In this study, kinetic features interaction RH1 from

Language: Английский

Citations

0