Biomacromolecules,
Journal Year:
2024,
Volume and Issue:
26(1), P. 363 - 373
Published: Dec. 2, 2024
Accurate
determination
of
biomolecular
condensate
volume
reveals
that
destabilization
condensates
can
lead
to
either
swelling
or
shrinking
condensates,
giving
fundamental
insights
into
the
regulation
cellular
condensates.
Determination
and
coacervate
protocells
is
essential
investigate
their
precise
composition
impact
on
(bio)chemical
reactions
are
localized
inside
It
not
a
straightforward
task,
as
have
tiny
volumes,
highly
viscous,
prone
wetting.
Here,
we
examine
different
strategies
determine
introduce
two
new
methods,
with
which
volumes
1
μL
less
(volume
fraction
0.4%)
be
determined
standard
deviation
0.03
μL.
Using
these
show
depends
degree
physical
cross-linking.
These
observations
supported
by
Flory–Huggins
theory
profound
effects
in
cell
biology.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 10, 2024
Macroautophagy
(hereafter
autophagy)
is
essential
for
cells
to
respond
nutrient
stress
by
delivering
cytosolic
contents
vacuoles
degradation
via
the
formation
of
a
multi-layer
vesicle
named
autophagosome.
A
set
autophagy-related
(ATG)
regulators
are
recruited
phagophore
assembly
site
initiation
phagophore,
as
well
its
expansion
and
closure
subsequent
delivery
into
vacuole.
However,
it
remains
elusive
that
how
regulated
under
different
conditions.
Here,
we
described
an
unknown
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Sept. 28, 2024
Abstract
Enzymatic
activity
and
its
tight
regulation
are
fundamental
for
cellular
metabolism
life.
While
classical
models
of
enzyme
kinetics
explain
the
behaviour
enzymes
in
buffer
solutions,
there
elusive
properties
that
emerge
from
their
native,
crowded
environment.
In
this
study,
we
harness
liquid-liquid
phase
separation
(LLPS)
to
create
vitro
droplets
mimic
cytosolic
protein
crowding,
offering
a
unique
window
understand
within
well
controlled
microenvironments.
We
discover
self-regulates
by
modifying
shear
viscosity
both
protein-rich
surrounding
bulk
solution.
Through
microscopy
rheology
techniques,
discovered
significant
correlation
between
solution,
influencing
substrate
diffusion.
Our
findings
suggest
control
activity,
organization
dynamics
macromolecular
crowding
droplets.
These
results
provide
new
insights
into
how
regulate
physical
environment
metabolic
processes
cell.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: Nov. 8, 2024
Abstract
Timely
and
precise
assembly
of
protein
complexes
on
membrane
surfaces
is
essential
to
the
physiology
living
cells.
Recently,
phase
separation
has
been
observed
at
cellular
membranes,
suggesting
it
may
play
a
role
in
complexes.
Inspired
by
these
findings,
we
that
condensates
one
side
planar
suspended
spontaneously
colocalized
with
those
opposite
side.
How
might
this
phenomenon
contribute
stable
transmembrane
complexes?
To
address
question,
examined
diffusion
growth
both
sides
membranes.
Our
results
reveal
coupling
slows
down
condensate
while
accelerating
growth.
can
rate
increase
simultaneously
decrease
diffusion?
We
provide
insights
into
seemingly
contradictory
observations
distinguishing
between
diffusion-limited
coupling-driven
processes.
While
diffusion,
also
locally
concentrates
within
confined
area.
This
confinement
increases
probability
coalescence
thereby
enhances
overall
for
coupled
condensates,
substantially
surpassing
uncoupled
condensates.
These
findings
suggest
could
diverse
membrane-bound
structures
promoting
localization
surfaces.
help
explain
efficient
contexts.
Significance
Protein
assemblies
span
biological
membranes
are
critical
physiology.
In
past
decade,
liquid-like
which
flexible,
multivalent
assemblies,
have
discovered
colocalize
form
coupled,
Interestingly,
accelerates
their
strongly
localizing
interactions
them.
robust
crowded,
complex
environments.
Biomacromolecules,
Journal Year:
2024,
Volume and Issue:
26(1), P. 363 - 373
Published: Dec. 2, 2024
Accurate
determination
of
biomolecular
condensate
volume
reveals
that
destabilization
condensates
can
lead
to
either
swelling
or
shrinking
condensates,
giving
fundamental
insights
into
the
regulation
cellular
condensates.
Determination
and
coacervate
protocells
is
essential
investigate
their
precise
composition
impact
on
(bio)chemical
reactions
are
localized
inside
It
not
a
straightforward
task,
as
have
tiny
volumes,
highly
viscous,
prone
wetting.
Here,
we
examine
different
strategies
determine
introduce
two
new
methods,
with
which
volumes
1
μL
less
(volume
fraction
0.4%)
be
determined
standard
deviation
0.03
μL.
Using
these
show
depends
degree
physical
cross-linking.
These
observations
supported
by
Flory–Huggins
theory
profound
effects
in
cell
biology.
