Genomic and cellular contributions to establish the fish Hyphessobrycon heterorhabdus as an Amazonian model for ecotoxicology

Environmental Toxicology and Chemistry, Journal Year: 2025, Volume and Issue: 44(1), P. 146 - 158

Published: Jan. 1, 2025

Abstract The Amazon has environmental characteristics that make it unique, and the species inhabit have physiological features demonstrate their adaptation to an Amazonian context. ecosystems been undergoing transformations result from inadequate human actions, which placed biodiversity at limits of biological abilities. Therefore, is essential identify organisms reflect conditions environment, considering physiology these species, they are established as bioindicators for this region. fish Hyphessobrycon heterorhabdus distributed throughout lower middle basin. Aiming contribute establishment a bioindicator, we carried out first time its karyotyping, Illumina sequencing assembly nuclear genome mitogenome, in addition establishing cell line species. Individuals H. 48 chromosomes, number held line. Sequencing genomic generated draft useful recovering species-specific coding regulatory sequences. was responsive variables, reflecting vivo observations. Thus, possible present set resources (standard karyotype, centromeric marker, genome, line) applications relevance ecotoxicology. toolkit should further studies on response natural impacts region receiving.

Language: Английский

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Enteric neural crest development in Astyanax mexicanus surface fish and cavefish

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

The enteric nervous system (ENS) regulates gastrointestinal (GI) functions such as secretion, blood flow, and motility, yet how its structure function evolve with dietary adaptations remains unclear. Astyanax mexicanus , a teleost fish surface cave morphotypes, offers model to explore these changes; cavefish exhibit altered GI motility transit that may help them adapt their unique diet. We compared early ENS development in cavefish, tracking neural crest cell (ENCC) migration differentiation using phox2bb HuC/D markers. found ENCCs reach the gut by 36 hours post-fertilization (hpf) both morphotypes but migrate differentiate more rapidly along tube cavefish. To genetic basis of this difference, we used available genomic datasets compare predicted peptide sequences genes important for other species identified mutations could impact protein function, example endothelin signaling ENCC differentiation. specifically examined expression endothelin-3 (edn3) receptor-b (ednrb) during localization edn3 not ednrb is consistent role development. Overall, our findings position A. studying evolution

Language: Английский

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Fish as biological models: Implications for gut microbiota research in biomedical and aquaculture sciences

Aquaculture International, Journal Year: 2025, Volume and Issue: 33(4)

Published: May 2, 2025

Language: Английский

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Host evolution shapes gut microbiome composition in Astyanax mexicanus

Ecology and Evolution, Journal Year: 2024, Volume and Issue: 14(4)

Published: April 1, 2024

The ecological and genetic changes that underlie the evolution of host-microbe interactions remain elusive, primarily due to challenges in disentangling variables alter microbiome composition. To understand impact host habitat, genetics, evolutionary history on microbial community structure, we examined gut microbiomes river- three cave-adapted morphotypes Mexican tetra,

Language: Английский

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Hypoxia-Sonic Hedgehog Axis as a Driver of Primitive Hematopoiesis Development and Evolution in Cavefish

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 9, 2024

The teleost

Language: Английский

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Variable Craniofacial Shape and Development among Multiple Cave-Adapted Populations of Astyanax mexicanus

Integrative Organismal Biology, Journal Year: 2024, Volume and Issue: 6(1)

Published: Jan. 1, 2024

Synopsis Astyanax mexicanus is a freshwater fish species with blind cave morphs and sighted surface morphs. Like other troglodytic species, independently evolved cave-dwelling A. populations share several stereotypic phenotypes, including the expansion of certain sensory systems, as well loss eyes pigmentation. Here, we assess extent to which there also parallelism in craniofacial development across populations. Since multiple forces may be acting upon variation system, phylogenetic history, selection, developmental constraint, outcomes are possible. For example, eye regression have triggered conserved series compensatory events, case would expect observe highly similar phenotypes Selection for cave-specific foraging lead evolution phenotype, especially regions head directly associated feeding. Alternatively, absence common axis selection or strong constraints, shape evolve under neutral processes such gene flow, drift, bottlenecking, patterns should reflect evolutionary history mexicanus. Our results found that cave-adapted do anatomical features; however, they generally did not support hypothesis phenotype caves, nearly every pairwise comparison was statistically significant, greater effect sizes noted between more distantly related little flow. A pattern observed trajectories. We morphological disparity lower among all three versus fish, suggesting increased variation, consistent release constraint. Instead, this reflects relatively low genetic diversity within Finally, magnitudes integration were groups, meaning coordinated units robust conclude that, contrast many populations, global variable, line population structure than architecture selection.

Language: Английский

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Hypoxia-sonic hedgehog axis as a driver of primitive hematopoiesis development and evolution in cavefish

Developmental Biology, Journal Year: 2024, Volume and Issue: 516, P. 138 - 147

Published: Aug. 22, 2024

Language: Английский

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