Plant microtubule nucleating apparatus and its potential signaling pathway DOI Creative Commons
Noriyoshi Yagi, Satoshi Fujita, Masayoshi Nakamura

et al.

Current Opinion in Plant Biology, Journal Year: 2024, Volume and Issue: 82, P. 102624 - 102624

Published: Sept. 3, 2024

Plant cell cortical microtubules are located beneath the plasma membrane and direct location of cellulose synthases during interphase, influencing morphology. Microtubule-associated proteins (MAPs) regulate these in response to growth environmental stimuli. This review focuses on recent advances understanding microtubule nucleation mechanisms plants spatiotemporal regulation arrays via phytohormone signaling. Emphasis is placed conserved nature gamma-tubulin ring complex (γTuRC) plant-specific components. The discussion includes role Augmin distinct function Msd1-Wdr8 plants. We also explore effects hormone signaling, particularly brassinosteroids, regulatory apparatus. interplay between signaling pathways dynamics, including phosphorylation events post-translational modifications, addressed. Finally, impact signals protein modifications regulating organization suggested for future research.

Language: Английский

Transcriptomic profiling revealed the regulatory pathways and key genes associated with cold tolerance in two eel gobies DOI
Jing Liu, Yantao Liu,

Tianwei Liu

et al.

Journal of Thermal Biology, Journal Year: 2025, Volume and Issue: 130, P. 104136 - 104136

Published: May 1, 2025

Language: Английский

Citations

0
Tubulin code eraser CCP5 binds branch glutamates by substrate deformation DOI
Jiayi Chen, Elena A. Zehr, James M. Gruschus

et al.

Nature, Journal Year: 2024, Volume and Issue: 631(8022), P. 905 - 912

Published: July 17, 2024

Language: Английский

Citations

3
Polyglutamylation of microtubules drives neuronal remodeling DOI Open Access
Antoneta Gavoci,

Anxhela Zhiti,

Michaela Rusková

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 12, 2024

Abstract Developmental remodeling shapes neural circuits via activity-dependent pruning of synapses and axons. The cytoskeleton is critical for this process, as microtubule loss enzymatic severing an early step across many species. However, how microtubule-severing enzymes, such spastin, are activated in specific neuronal compartments remains unknown. Here, we reveal that polyglutamylation, a posttranslational tubulin modification enriched neurons, plays instructive role developmental by tagging microtubules severing. Motor neuron-specific gene deletion enzymes add or remove polyglutamylation—TTLL glutamylases vs. CCP deglutamylases—accelerates delays neuromuscular synapse neurotransmission-dependent manner. This mechanism not to peripheral but also operates central circuits, e.g., the hippocampus. Thus, polyglutamylation acts rheostat morphology connectivity.

Language: Английский

Citations

2
Utilizing codon degeneracy in the design of donor DNA for CRISPR/Cas9‐mediated gene editing to streamline the screening process for single amino acid mutations DOI
Xuecheng Li, Yongli Zhang, Xin Wen

et al.

The Plant Journal, Journal Year: 2024, Volume and Issue: 119(4), P. 2133 - 2143

Published: July 4, 2024

SUMMARY Chlamydomonas reinhardtii , a unicellular green alga, has been widely used as model organism for studies of algal, plant and ciliary biology. The generation targeted amino acid mutations is often necessary, this can be achieved using CRISPR/Cas9 induced homology‐directed repair to install genomic modifications from exogenous donor DNA. Due the low gene editing efficiency, technical challenge lies in identifying mutant cells. Direct sequencing not practical, pre‐screening required. Here, we report strategy generating screening point system. based on designing DNA codon degeneracy, which enables design specific primers facilitate by PCR. An vitro assembled RNP complex, along with dsDNA an antibiotic resistance marker, was electroporated into wild‐type cells, followed PCR screening. To demonstrate principle, have generated E102K mutation centrin K40R α‐tubulin. efficiencies at target sites Centrin TUA1 TUA2 were 4, 24 8% respectively, More than 80% mutants expected size products precisely edited, revealed sequencing. Subsequently, precision‐edited biochemically verified. introduction degeneracy did affect expression α‐tubulins. Thus, approach identification mutations, especially genes rates.

Language: Английский

Citations

2
Plant microtubule nucleating apparatus and its potential signaling pathway DOI Creative Commons
Noriyoshi Yagi, Satoshi Fujita, Masayoshi Nakamura

et al.

Current Opinion in Plant Biology, Journal Year: 2024, Volume and Issue: 82, P. 102624 - 102624

Published: Sept. 3, 2024

Plant cell cortical microtubules are located beneath the plasma membrane and direct location of cellulose synthases during interphase, influencing morphology. Microtubule-associated proteins (MAPs) regulate these in response to growth environmental stimuli. This review focuses on recent advances understanding microtubule nucleation mechanisms plants spatiotemporal regulation arrays via phytohormone signaling. Emphasis is placed conserved nature gamma-tubulin ring complex (γTuRC) plant-specific components. The discussion includes role Augmin distinct function Msd1-Wdr8 plants. We also explore effects hormone signaling, particularly brassinosteroids, regulatory apparatus. interplay between signaling pathways dynamics, including phosphorylation events post-translational modifications, addressed. Finally, impact signals protein modifications regulating organization suggested for future research.

Language: Английский

Citations

2