Cited by Polarity-dependent expression and localization of secretory glucoamylase mRNA in filamentous fungal cells

In situ cellular hitchhiking of nanoparticles for drug delivery DOI

Edidiong Udofa, Zongmin Zhao

Advanced Drug Delivery Reviews, Journal Year: 2023, Volume and Issue: 204, P. 115143 - 115143

Published: Nov. 24, 2023

Language: Английский

Citations

Inducing novel endosymbioses by implanting bacteria in fungi DOI

Gabriel H. Giger,

Chantal Ernst,

Ingrid Richter

et al.

Nature, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 2, 2024

Language: Английский

Citations

Neurons dispose of hyperactive kinesin into glial cells for clearance DOI

Chao Xie, Guanghan Chen, Ming Li

et al.

The EMBO Journal, Journal Year: 2024, Volume and Issue: 43(13), P. 2606 - 2635

Published: May 28, 2024

Microtubule-based kinesin motor proteins are crucial for intracellular transport, but their hyperactivation can be detrimental cellular functions. This study investigated the impact of a constitutively active ciliary mutant, OSM-3CA, on sensory cilia in C. elegans. Surprisingly, we found that OSM-3CA was absent from underwent disposal through membrane abscission at tips aberrant neurites. Neighboring glial cells engulf and eliminate released process depends engulfment receptor CED-1. Through genetic suppressor screens, identified intragenic mutations domain inhibiting kinase DYF-5, both which restored normal OSM-3CA-expressing animals. We showed conformational changes prevent its entry into cilia, requires hyperactivity. Finally, provide evidence neurons also dispose hyperactive kinesin-1 resulting clinic variant associated with amyotrophic lateral sclerosis, suggesting widespread mechanism regulating kinesins.

Language: Английский

Citations

Microtubule Regulation in Plants: From Morphological Development to Stress Adaptation DOI

An‐Shan Hsiao,

Ji-Ying Huang

Biomolecules, Journal Year: 2023, Volume and Issue: 13(4), P. 627 - 627

Published: March 30, 2023

Microtubules (MTs) are essential elements of the eukaryotic cytoskeleton and critical for various cell functions. During division, plant MTs form highly ordered structures, cortical guide wall cellulose patterns thus control size shape. Both important morphological development adjusting growth plasticity under environmental challenges stress adaptation. Various MT regulators dynamics organization in diverse cellular processes response to developmental cues. This article summarizes recent progress studies from responses, discusses latest techniques applied, encourages more research into regulation.

Language: Английский

Citations

Polarity-dependent expression and localization of secretory glucoamylase mRNA in filamentous fungal cells DOI

Yuki Morita,

Kaoru Takegawa, Brett M. Collins

et al.

Microbiological Research, Journal Year: 2024, Volume and Issue: 282, P. 127653 - 127653

Published: Feb. 23, 2024

In multinuclear and multicellular filamentous fungi little is known about how mRNAs encoding secreted enzymes are transcribed localized spatiotemporally. To better understand this process we analyzed mRNA GlaA, a glucoamylase in large amounts by the industrial fungus Aspergillus oryzae, MS2 system, which can be visualized living cells. We found that glaA was significantly near hyphal tip septum, sites of protein secretion, polarity-dependent expression localization manners. also revealed exhibits long-range dynamics vicinity endoplasmic reticulum (ER) manner dependent on microtubule motor proteins kinesin-1 kinesin-3, but independent early endosomes. Moreover, elucidated although to stress granules (SGs) processing bodies (PBs) under high temperature, not seen ER stress, suggesting there different regulatory mechanisms SG PB temperature stress. Collectively, study uncovers dynamic mechanism secretory enzyme fungi.

Language: Английский

Citations

The Wheat NLR Protein PM3b Localises to Endoplasmic Reticulum–Plasma Membrane Contact Sites and Interacts With AVRPM3^b2/c2 Through Its LRR Domain DOI

Jonatan Isaksson, Lukas Kunz, Simon Flueckiger

et al.

Molecular Plant Pathology, Journal Year: 2025, Volume and Issue: 26(2)

Published: Feb. 1, 2025

ABSTRACT Plant nucleotide‐binding leucine‐rich repeat (NLR) proteins are intracellular immune receptors that directly or indirectly perceive pathogen‐derived effector to induce an response. NLRs display diverse subcellular localisations, which associated with the capacity of receptor confer disease resistance and recognise its corresponding avirulence effector. In wheat, NLR PM3b recognises wheat powdery mildew AVRPM3 b2/c2 we examined molecular mechanism underlying this recognition. We show other PM3 variants localise endoplasmic reticulum (ER)–plasma membrane (PM) contact sites (EPCS), while localises nucleocytoplasmic space. Additionally, found interacts in planta through LRR domain. further demonstrate full‐length interaction is considerably weaker than for isolated domain susceptible variant PM3CS, indicating activation leads dissociation complex. line this, observed a strong between P‐loop mutant was unable initiate cell death response, when inactive used. propose transiently residues conserved among variants, amino acids necessary full signalling unique PM3b. Our data suggests localisation differ from well‐studied highlights mechanistic diversity NLR‐mediated responses against pathogens plants.

Language: Английский

Citations

Acyl-coA binding protein AcbdA regulates peroxisome hitchhiking on early endosomes DOI

B. Driscoll,

Michelle D. Fountain,

Isabella N. Gates

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 23, 2025

Motor-driven transport on microtubules is critical for distributing organelles throughout the cell. Most commonly, organelle movement mediated by cargo adaptors, proteins surface of an that directly recruit microtubule-based motors. An alternative mechanism called hitchhiking was recently discovered: some move, not recruiting motors directly, but instead using membrane contact sites to attach motor-driven vesicles and hitchhike along microtubules. Organelle observed across fungi animals. In filamentous fungi, nearly all peroxisomes move early endosomes (EEs). fungus Aspergillus nidulans , EE-associated linker PxdA DipA are establishing EE-peroxisome required peroxisome movement. How recognize this subset EEs what peroxisome-membrane exist can interact with known. Here, we undertook a forward mutagenesis screen identify such proteins. We discovered acyl-coA binding (ACB) domain-containing protein AcbdA/AN1062 localizes via its tail-anchored transmembrane domain (TMD). Deleting AcbdA gene or only N-terminal ACB perturbs distribution peroxisomes. Importantly, recruitment EEs. Fatty acid (FA)-induced increases in require AcbdA, suggesting coupled FA metabolism. Mutating conserved FFAT motif, predicted endoplasmic reticulum (ER), has no effect Taken together, our data indicate tether during hitchhiking. AcbdA’s involvement peroxisome-EE site formation represents divergence from known functions Acbd4/5 adds layers understanding functionality family

Language: Английский

Citations

Phosphorylation-dependent regional motility of the ciliary kinesin OSM-3 DOI

Peng Huang, Guanghan Chen, Zhiwen Zhu

et al.

The Journal of Cell Biology, Journal Year: 2025, Volume and Issue: 224(7)

Published: April 24, 2025

Kinesin motor proteins, vital for intracellular microtubule-based transport, display region-specific motility within cells, a phenomenon that remains molecularly enigmatic. This study focuses on the localized activation of OSM-3, an intraflagellar transport kinesin crucial assembly ciliary distal segments in Caenorhabditis elegans sensory neurons. Fluorescence lifetime imaging microscopy unveiled extended, active conformation OSM-3 base and middle segments, where is conveyed as cargo by kinesin-II. We demonstrate NEKL-3, never mitosis kinase-like protein, directly phosphorylates domain inhibiting its vitro activity. NEKL-3 NEKL-4, at base, function redundantly to restrict activation. Elevated levels protein phosphatase 2A transition zone or triggered premature motility, while deficiency resulted reduced activity shorter cilia. These findings elucidate phosphorylation-mediated mechanism governing regional kinesins.

Language: Английский

Citations

Kinesin Autoinhibition Requires Elbow Phosphorylation DOI

Guanghan Chen, Zhengyang Guo, Zhiwen Zhu

et al.

Published: May 15, 2025

Abstract Kinesin motor proteins facilitate microtubule-based transport by converting chemical energy into mechanic forces, but this activity is autoinhibited until cargo loaded. Regulatory mechanisms underlying autoinhibitory conformation are not well understood. Here, we show that a NEver in mitosis Kinase NEKL-3 directly phosphorylates flexible elbow region between two coiled coil domains connecting the head and tail of an intraflagellar kinesin, OSM-3. The phosphor dead (PD) mutation, mimic (PM) induce constitutive motility OSM-3 vitro. Using knock-in animals, discovered both PD PM mutations shorten C. elegans sensory cilia. constitutively active OSM-3PD fails to enter cilia abnormally accumulates neurites, mimicking another hyperactive OSM-3G444E. Conversely, OSM-3PM enters moves at reduced speed, indicating inhibitory role phosphorylation kinesin motility. These findings highlight crucial regulating autoinhibition.

Language: Английский

Citations

Woronin body hitchhiking on early endosomes is dispensable for septal localization in Aspergillus nidulans DOI

Livia D. Songster,

Devahuti Bhuyan,

Jenna R. Christensen

et al.

Molecular Biology of the Cell, Journal Year: 2023, Volume and Issue: 34(7)

Published: April 5, 2023

The proper functioning of organelles depends on their intracellular localization, mediated by motor protein-dependent transport cytoskeletal tracks. Rather than directly associating with a protein, peroxisomes move hitchhiking motile early endosomes in the filamentous fungus

Language: Английский

Citations