Nature Aging, Journal Year: 2024, Volume and Issue: 4(2), P. 213 - 230
Published: Jan. 17, 2024
Language: Английский
Nature Cell Biology, Journal Year: 2021, Volume and Issue: 23(4), P. 424 - 436
Published: April 1, 2021
Language: Английский
Citations
188
Trends in Biochemical Sciences, Journal Year: 2021, Volume and Issue: 46(10), P. 790 - 804
Published: May 27, 2021
Language: Английский
Citations
152
Science, Journal Year: 2021, Volume and Issue: 372(6546), P. 1057 - 1062
Published: June 3, 2021
It is widely hypothesized that removing cellular transfer RNAs (tRNAs)-making their cognate codons unreadable-might create a genetic firewall to viral infection and enable sense codon reassignment. However, it has been impossible test these hypotheses. In this work, following synonymous compression laboratory evolution in Escherichia coli, we deleted the tRNAs release factor 1, which normally decode two stop codon; resulting cells could not read canonical code were completely resistant cocktail of viruses. We reassigned efficient synthesis proteins containing three distinct noncanonical amino acids. Notably, demonstrate facile reprogramming our for encoded translation diverse heteropolymers macrocycles.
Language: Английский
Citations
148
Nature Reviews Genetics, Journal Year: 2022, Volume and Issue: 23(11), P. 651 - 664
Published: June 9, 2022
Language: Английский
Citations
114
Annual Review of Plant Biology, Journal Year: 2023, Volume and Issue: 74(1), P. 21 - 51
Published: March 1, 2023
Plant cells accumulate small RNA molecules that regulate plant development, genome stability, and environmental responses. These RNAs fall into three major classes based on their function mechanisms of biogenesis—microRNAs, heterochromatic interfering RNAs, secondary RNAs—plus several other less well-characterized categories. Biogenesis each class requires a pathway factors, some specific to others involved in multiple pathways. Diverse sequenced genomes, along with rapid developments sequencing, imaging, genetic transformation techniques, have enabled significant progress understanding the biogenesis, functions, evolution including those had been poorly characterized because they were absent or low representation Arabidopsis ( thaliana). Here, we review recent findings about discuss our current biogenesis mechanisms, targets, modes action, mobility, functions species, economically important crops.
Language: Английский
Citations
109
Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)
Published: April 20, 2022
Abstract RNA modifications are important regulatory elements of functions. However, most genome-wide mapping has focused on messenger RNAs and transfer RNAs, but such datasets have been lacking for small RNAs. Here we mapped N 1 -methyladenosine (m A) in the cellular space. Benchmarked with synthetic m A our workflow identified specific groups A-containing which otherwise disproportionally under-represented. In particular, 22-nucleotides long 3′ tRNA-fragments highly enriched TRMT6/61A-dependent located within seed region. negatively affects gene silencing by tRF-3s. urothelial carcinoma bladder, where TRMT6/61A is over-expressed, higher modification tRFs detected, correlated a dysregulation tRF targetome. Lastly, regulates tRF-3 targets involved unfolded protein response. Together, results reveal mechanism regulating expression via base RNA.
Language: Английский
Citations
86
Nature Reviews Microbiology, Journal Year: 2022, Volume and Issue: 21(1), P. 21 - 34
Published: Sept. 28, 2022
Language: Английский
Citations
74
Molecular Cancer, Journal Year: 2023, Volume and Issue: 22(1)
Published: July 29, 2023
Abstract Newly growing evidence highlights the essential role that epitranscriptomic marks play in development of many cancers; however, little is known about and implications altered epitranscriptome deposition prostate cancer. Here, we show transfer RNA N 7 -methylguanosine (m G) transferase METTL1 highly expressed primary advanced tumours. Mechanistically, find depletion causes loss m G tRNA methylation promotes biogenesis a novel class small non-coding RNAs derived from 5'tRNA fragments. 5'tRNA-derived steer translation control to favour synthesis key regulators tumour growth suppression, interferon pathway, immune effectors. Knockdown Mettl1 cancer preclinical models increases intratumoural infiltration pro-inflammatory cells enhances responses immunotherapy. Collectively, our findings reveal therapeutically actionable METTL1-directed cell biology.
Language: Английский
Citations
54
Molecular Cell, Journal Year: 2023, Volume and Issue: 83(22), P. 3953 - 3971
Published: Oct. 5, 2023
tRNA function is based on unique structures that enable mRNA decoding using anticodon trinucleotides. These interact with specific aminoacyl-tRNA synthetases and ribosomes 3D shape sequence signatures. Beyond translation, tRNAs serve as versatile signaling molecules interacting other RNAs proteins. Through evolutionary processes, fragmentation emerges not merely random degradation but an act of recreation, generating shorter called tRNA-derived small (tsRNAs). tsRNAs exploit their linear sequences newly arranged for unexpected biological functions, epitomizing the "renovatio" (from Latin, meaning renewal, renovation, rebirth). Emerging methods to uncover full tRNA/tsRNA modifications, combined techniques study RNA integrate AI-powered predictions, will comprehensive investigations products new interaction potentials in relation functions. We anticipate these directions herald a era understanding complexity advancing pharmaceutical engineering.
Language: Английский
Citations
46
Nature Reviews Molecular Cell Biology, Journal Year: 2024, Volume and Issue: 25(5), P. 359 - 378
Published: Jan. 5, 2024
Language: Английский
Citations
30