A light-induced microprotein triggers regulated intramembrane proteolysis to promote photo sensing in a pathogenic bacterium. DOI Creative Commons

Dimitrios Manias,

Jonathan D. Winkelman, Gong Chen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

ABSTRACT Light, a ubiquitous environmental stimulus, shapes behavior and physiology across all domains of life. While photoreceptors are widespread in bacterial genomes, their functional roles signal transduction mechanisms non-photosynthetic bacteria remain understudied. Light represses biofilms virulence factors through bacteriophytochrome photoreceptor BphP response regulator AlgB the human pathogen Pseudomonas aeruginosa . Here, we used transposon mutagenesis screening to identify conserved hypothetical microprotein, DimA, as master activator photo-sensing cascade. Transcriptomics, luciferase reporter assays physiological revealed that deletion dimA abolishes light-dependent suppression biofilms. Mechanistically, demonstrated DimA activates site-I protease AlgW, triggering regulated intramembrane proteolysis anti-sigma factor MucA, liberating sigma AlgU (σ22), which promotes algB expression. We discovered positive feedback loop where light-activated upregulates expression, thereby amplifying photosensory response. This work establishes crucial photo sensing expands our understanding adaptation changing light environments.

Language: Английский

Treponema denticola major surface protein (Msp): a key player in periodontal pathogenicity and immune evasion DOI
Yue Zhao, Jiaxin Chen,

Yifei Tian

et al.

Archives of Microbiology, Journal Year: 2025, Volume and Issue: 207(2)

Published: Jan. 18, 2025

Language: Английский

Citations

0
Molecular mechanism of selenite reduction by Bacillus amyloliquefaciens BB61 based on transcriptome analysis DOI
Yujie Wang, Fan Wan,

Huiqin Xue

et al.

Biochimie, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
A light-induced microprotein triggers regulated intramembrane proteolysis to promote photo sensing in a pathogenic bacterium. DOI Creative Commons

Dimitrios Manias,

Jonathan D. Winkelman, Gong Chen

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

ABSTRACT Light, a ubiquitous environmental stimulus, shapes behavior and physiology across all domains of life. While photoreceptors are widespread in bacterial genomes, their functional roles signal transduction mechanisms non-photosynthetic bacteria remain understudied. Light represses biofilms virulence factors through bacteriophytochrome photoreceptor BphP response regulator AlgB the human pathogen Pseudomonas aeruginosa . Here, we used transposon mutagenesis screening to identify conserved hypothetical microprotein, DimA, as master activator photo-sensing cascade. Transcriptomics, luciferase reporter assays physiological revealed that deletion dimA abolishes light-dependent suppression biofilms. Mechanistically, demonstrated DimA activates site-I protease AlgW, triggering regulated intramembrane proteolysis anti-sigma factor MucA, liberating sigma AlgU (σ22), which promotes algB expression. We discovered positive feedback loop where light-activated upregulates expression, thereby amplifying photosensory response. This work establishes crucial photo sensing expands our understanding adaptation changing light environments.

Language: Английский

Citations

0