The Impact of Fermented Milk Products Containing Bifidobacterium longum BB536 on the Gut Environment: A Randomized Double-Blind Placebo-Controlled Trial DOI Open Access
Ryuta Ejima, Riko Mishima,

Akira Sen

et al.

Nutrients, Journal Year: 2024, Volume and Issue: 16(21), P. 3580 - 3580

Published: Oct. 22, 2024

Probiotics, particularly those native to the gut microbiota, have a profound influence on environment. In this study, we conducted randomized placebo-controlled, double-blind, parallel-group comparison trial investigate effects of Bifidobacterium longum BB536 (B. BB536) fecal microbiota and metabolite compositions in healthy individuals. We compared fermented milk produced solely with Streptococcus thermophiles Lactobacillus bulgaricus (placebo group) supplemented B. (BY group). Our findings revealed significantly greater relative abundance Faecalibacterium BY group than placebo by 3rd day, trend that persisted until end 17th day. Additionally, presented increased concentrations tryptophan (Trp), Indole-3-lactic acid, Indole-3-aldehyde A significant positive correlation was observed between number viable bacteria feces. The Trp Indole-3-acetic acid were also correlated results suggest intake can modulate profiles, which are general indicators for monitoring environment, potentially conferring health benefits host.

Language: Английский

Gut microbiota and their metabolites in the immune response of rheumatoid arthritis: Therapeutic potential and future directions DOI
Xiaoyu Cai,

Fujia Ren,

Yao Yao

et al.

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 147, P. 114034 - 114034

Published: Jan. 12, 2025

Language: Английский

Citations

2
The tryptophan metabolic pathway of the microbiome and host cells in health and disease DOI Creative Commons
Kentaro Miyamoto, Tomohisa Sujino, Takanori Kanai∥

et al.

International Immunology, Journal Year: 2024, Volume and Issue: unknown

Published: June 13, 2024

Abstract The intricate and dynamic tryptophan (Trp) metabolic pathway in both the microbiome host cells highlights its profound implications for health disease. This involves complex interactions between cellular bacteria processes, producing bioactive compounds such as 5-hydroxytryptamine (5-HT) kynurenine derivatives. Immune responses to Trp metabolites through specific receptors have been explored, highlighting role of aryl hydrocarbon receptor inflammation modulation. Dysregulation this is implicated various diseases, Alzheimer’s Parkinson’s mood disorders, neuronal autoimmune diseases multiple sclerosis (MS), cancer. In article, we describe impact 5-HT, Trp, indole, on Furthermore, review microbiome-derived that affect immune contribute maintaining homeostasis, especially an experimental encephalitis model MS.

Language: Английский

Citations

8
The Biology and Biochemistry of Kynurenic Acid, a Potential Nutraceutical with Multiple Biological Effects DOI Open Access
Luana de Fátima Alves, J. Bernadette Moore, Douglas B. Kell

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(16), P. 9082 - 9082

Published: Aug. 21, 2024

Kynurenic acid (KYNA) is an antioxidant degradation product of tryptophan that has been shown to have a variety cytoprotective, neuroprotective and neuronal signalling properties. However, mammalian transporters receptors display micromolar binding constants; these are consistent with its typically tissue concentrations but far above serum/plasma concentration (normally tens nanomolar), suggesting large gaps in our knowledge transport mechanisms action, the main influx characterized date equilibrative, not concentrative. In addition, it substrate known anion efflux pump (ABCC4), whose vivo activity largely unknown. Exogeneous addition L-tryptophan or L-kynurenine leads production KYNA also many other co-metabolites (including some such as 3-hydroxy-L-kynurenine quinolinic may be toxic). With exception chestnut honey, exists at relatively low levels natural foodstuffs. bioavailability reasonable, terminal element irreversible reaction most pathways, might added exogenously without disturbing upstream metabolism significantly. Many examples, which we review, show valuable bioactivity. Given above, review potential utility nutraceutical, finding significantly worthy further study development.

Language: Английский

Citations

6
The Association Between Branched-Chain Amino Acid Concentrations and the Risk of Autism Spectrum Disorder in Preschool-Aged Children DOI Creative Commons
Qi Gao, Dan Bi, Bingbing Li

et al.

Molecular Neurobiology, Journal Year: 2024, Volume and Issue: 61(8), P. 6031 - 6044

Published: Jan. 24, 2024

Several studies have linked branched-chain amino acid (BCAA) metabolism disorders with autism spectrum disorder (ASD), but the results been inconsistent. The purpose of this study was to explore association between BCAA concentrations and risk ASD. A total 313 participants were recruited from two tertiary referral hospitals May 2018 July 2021. Concentrations BCAAs in dried blood spots analyzed using liquid chromatography-tandem mass spectrometry-based analysis. Multivariate analyses restricted cubic spline models used identify ASD, a nomogram developed by multivariate logistic regression determined receiver operating characteristic curve analysis calibration BCAA, valine, leucine/isoleucine higher ASD group, all them positively non-linearly associated even after adjusting for potential confounding factors such as age, gender, body index, (P < 0.05). integrating valine showed good discriminant AUC value 0.756 (95% CI 0.676-0.835). model could yield net benefits across reasonable range thresholds. In stratified analysis, diagnostic ability more pronounced children older than 3 years. We provide evidence that increased levels are presented here can serve marker early diagnosis

Language: Английский

Citations

4
Branched chain amino acid metabolism and microbiome in adolescents with obesity during weight loss therapy. DOI Open Access
Jessica R. McCann,

Chengxin Yang,

Nathan A. Bihlmeyer

et al.

medRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 4, 2025

ABSTRACT BACKGROUND Towards improving outcomes for adolescents with obesity, we aimed to define metabolic and microbiome phenotypes at baseline post-weight loss intervention. METHODS The Pediatric Obesity Microbiome Metabolism Study enrolled 220 aged 10-18 severe obesity (OB) 67 healthy weight controls (HWC). Blood, stool, clinical measures were collected after a 6-month intervention the OB group. Serum metabolomic fecal data analyzed associations BMI, insulin resistance, inflammation. Fecal transplants performed on germ-free mice using samples from both groups assess gain changes. RESULTS Adolescents exhibited elevated serum branched-chain amino acids (BCAA) but reduced ketoacid metabolites (BCKA) compared HWC. This pattern was sex- age-dependent, unlike adults OB, who showed levels of both. microbiomes HWC had similar diversity differed in membership functional potential. FMT donors effects mouse body weight, specific taxa linked recipients. Longitudinal analysis identified microbial features correlated changes health during CONCLUSION have unique adaptations signatures their counterparts OB. TRIAL REGISTRATION ClinicalTrials.gov Identifier: NCT03139877 (Observational Study) NCT02959034 (Repository) FUNDING SOURCES American Heart Association Grants: 17SFRN33670990, 20PRE35180195 National Institute Diabetes Digestive Kidney Diseases Grant: R24-DK110492

Language: Английский

Citations

0
The effect of microbial metabolites from colonic protein fermentation on bacteria‐induced cytokine production in dendritic cells DOI Creative Commons
Zhuqing Xie,

Danny Blichfeldt Eriksen,

Peter Riber Johnsen

et al.

BioFactors, Journal Year: 2025, Volume and Issue: 51(1)

Published: Jan. 1, 2025

Compared to the well-defined immune-modulating effect of butyrate, understanding other protein fermentation metabolites is limited. This study aimed investigate impact protein-derived (valerate, branched-chain fatty acids, ammonium, phenol, p-Cresol, indole, and hydrogen sulfide) on cytokine production in murine bone marrow-derived dendritic cells (BMDCs) stimulated with lipopolysaccharides (LPS), Lactobacillus acidophilus NCFM, or Staphylococcus aureus USA300. Some metabolites, but not short-chain acids (SCFAs), strongly affected cell viability. After short-term treatment depending microbial stimulus, SCFAs profile similarly weaker than as reflected by inhibition IL-12p70 IL-10 enhanced IL-23 (LPS S. USA300) IL-1β production. valerate exhibited a slower expression. Two-day butyrate resulted similar effects, that is, LPS-induced IL-12 abrogation enhancement, increased aryl hydrocarbon receptor (Ahr) expression, after LPS stimulation, expression dual specificity phosphatase 1 (Dusp1). In conclusion, low toxicity modulated microbially BMDCs. Valerate showed strongest effect, which was dependent specific stimulation course SCFA treatment. Our work adds knowledge regarding role from gut bacterial immune system.

Language: Английский

Citations

0
Bifidobacterium bifidum 1007478 derived indole-3-lactic acid alleviates NASH via an aromatic hydrocarbon receptor-dependent pathway in zebrafish DOI

Chao Tian,

Shizhou Deng, Zhao Zhang

et al.

Life Sciences, Journal Year: 2025, Volume and Issue: unknown, P. 123557 - 123557

Published: March 1, 2025

Language: Английский

Citations

0
Navigating nitrogen sustainability with microbiome-associated phenotypes DOI Creative Commons
Sierra S. Raglin, Angela D. Kent

Trends in Plant Science, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

HighlightsNitrogen fertilizer use has driven reactive nitrogen pools beyond safe limits, threatening environmental sustainability.Microbiome-associated phenotypes (MAPs) are microbially mediated traits that directed by the plant genome and contribute to health, including acquisition retention.MAPs aid biological fixation in non-leguminous crop species sustainable N acquisition.Biological nitrification denitrification inhibition additional MAPs can rhizosphere retention, but their effectiveness requires consideration of factors avoid unintended loss.Advancing MAP development through 'trait stacking' – strategically combining microbiome-associated enhance performance, should also strive ensure systemic adaptations needed for equitable genetic resources.AbstractCrop microbiomes promote health various mechanisms, nutrient provisioning. However, agriculture neglected importance these conventional management approaches originating from Green Revolution. Revolution innovations, such as fertilizers high-yielding germplasm, supported an increase global yields. Yet advances led many issues, disruptions transformations have reduced reliance on acquisition. Overcoming challenges introduced a shift toward ecologically informed agronomic strategies incorporate into breeding decisions. Agriculture Anthropocene needs mindfully manage decouple agrochemical inputs profitable yields, minimizing repercussions modern agriculture.

Language: Английский

Citations

0
Analysis of (p)ppGpp metabolism and signaling using a dynamic luminescent reporter DOI Creative Commons
Molly Hydorn, Sathya Narayanan Nagarajan, Elizabeth M. Fones

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 16, 2025

Abstract As rapidly growing bacteria begin to exhaust nutrients, their growth rate slows, ultimately leading the non-replicative state of quiescence. Adaptation nutrient limitation requires widespread metabolic remodeling that is in part mediated by phosphorylated nucleotides guanosine tetra- and penta-phosphate, collectively (p)ppGpp. We have developed a novel reporter (p)ppGpp abundance Gram-positive bacterium Bacillus subtilis based on recent identification riboswitch binds modulates transcription via regulation transcriptional terminator. Placement an unstable reporter, firefly luciferase, downstream allows for sensitive dynamic assessment first confirm accurately reflects variety well-established conditions. then proceed use it demonstrate physiological importance several mechanisms metabolism previously observed only vitro including allosteric interactions between synthesis enzymes hydrolytic activity synthase. signaling has been implicated gene expression, we close temporal association expression abundance, indicating rapid, therefore likely direct mechanism dependent activation. Thus, this provides new, comprehensive analysis vivo offers potential ability sensitively monitor dynamics under diverse environmental Author Summary Most adapt stressful conditions such as synthesizing molecule, known ppGpp, consists hyper-phosphorylated GTP. Synthesis ppGpp affects most aspects cellular physiology replication, translation. present here method time measurement living cells, greatly facilitating investigation into metabolism.

Language: Английский

Citations

0
Dietary amino acid manipulations in the common marmosetCallithrix jacchusimpacts serum metabolites and FGF21 levels DOI Creative Commons

Yuka Fujita,

Tomoko Ishibuchi,

Akiko Uematsu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: April 19, 2025

Abstract Restricting dietary protein intake has metabolic and physiological benefits for animals. Rodent studies have identified the involvement of a hormone fibroblast growth factor 21 (FGF21), which is upregulated by sensing amino acid scarcity. However, to what extent this mechanism conserved in primates remains elusive. Using common marmosets Callithrix jacchus as non-human primate model, we demonstrate protocol restriction manipulation. Low diet induces decrease blood urea nitrogen, altered plasma profiles, an increase FGF21. Supplementation purified acids suppresses features restriction. Our data provide intervention technique insight into evolutionarily FGF21 induction during

Language: Английский

Citations

0