Liquid biopsy-based protein biomarkers for risk prediction, early diagnosis, and prognostication of cholangiocarcinoma

Journal of Hepatology, Journal Year: 2023, Volume and Issue: 79(1), P. 93 - 108

Published: March 1, 2023

Language: Английский

---

Temporal Changes in Cholangiocarcinoma Incidence and Mortality in the United States from 2001 to 2017

The Oncologist, Journal Year: 2022, Volume and Issue: 27(10), P. 874 - 883

Published: Aug. 16, 2022

Abstract Background Previous studies report increasing cholangiocarcinoma (CCA) incidence up to 2015. This contemporary retrospective analysis of CCA and mortality in the US from 2001-2017 assessed whether continued increase beyond Patients Methods (≥18 years) with were identified National Cancer Institute Surveillance, Epidemiology, End Results 18 cancer registry (International Classification Disease for Oncology [ICD-O]-3 codes: intrahepatic [iCCA], C221; extrahepatic [eCCA], C240, C241, C249). unknown primary (CUP) cases (ICD-O-3: C809; 8140/2, 8140/3, 8141/3, 8143/3, 8147/3) because potential misclassification as iCCA. Forty-thousand-and-thirty (iCCA, n=13,174; eCCA, n=26,821; iCCA n=35) 32,980 CUP analyzed. From 2001-2017, CCA, iCCA, eCCA (per 100 000 person-years) increased 43.8% (3.08 4.43), 148.8% (0.80 1.99), 7.5% (2.28 2.45), respectively. In contrast, decreased 54.4% (4.65 2.12). age, greatest among younger patients (18-44 years, 81.0%). Median overall survival diagnosis was 8, 6, 9, 2 months CUP. 2001-2016, annual rate declined (57.1% 41.2%) generally remained stable (40.9% 37.0%) (64.3% 68.6%). Conclusions greater versus whereas decreased. The divergent trends, absolute change incidence, provide evidence a true that may not be wholly attributable reclassification.

Language: Английский

---

JUND/linc00976 promotes cholangiocarcinoma progression and metastasis, inhibits ferroptosis by regulating the miR-3202/GPX4 axis

Cell Death and Disease, Journal Year: 2022, Volume and Issue: 13(11)

Published: Nov. 18, 2022

Abstract Long noncoding RNAs (lncRNAs) are a novel class of that have emerged as critical regulators and biomarkers in various cancers. Nevertheless, the expression profile mechanistic function lncRNAs cholangiocarcinoma (CCA) remain unclear. Herein, we examined levels linc00976 clinical specimens cell lines using reverse transcription-quantitative PCR. In total, 50 patients with CCA were enrolled to analyze correlation between characteristics CCA. Loss- gain-of-function experiments performed investigate biological effects on proliferation, ferroptosis, migration, invasion cells vitro vivo. situ hybridization, RNA immunoprecipitation, bioinformatic databases, pull-down assay, dual-luciferase reporter mRNA sequencing, chromatin immunoprecipitation–PCR, rescue elucidate underlying mechanisms linc00976-induced competitive endogenous regulatory networks. We characterized abundant lncRNA, linc00976, functions pro-oncogenic regulator progression. Compared normal controls, was dramatically upregulated tissue samples lines. Patients exhibiting high had highly advanced stage, substantial lymph node metastasis, poor overall survival. Knockdown significantly repressed proliferation metastasis promoted ferroptosis both vivo, whereas overexpression exerted opposite effect. Mechanistically, competitively interacted miR-3202 upregulate GPX4 expression, thus contributing malignant behavior cells. Moreover, demonstrated JUND specifically interacts promoter activates transcription. Accordingly, promotes transcription, plays crucial role accelerating tumorigenesis inhibiting by modulating miR-3202/GPX4 axis. These findings suggest targeting may afford promising therapeutic strategy for

Language: Английский

---

Exploring the effects of gut microbiota on cholangiocarcinoma progression by patient-derived organoids

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 9, 2025

Language: Английский

---

Molecular profiling in cholangiocarcinoma: A practical guide to next-generation sequencing

Cancer Treatment Reviews, Journal Year: 2023, Volume and Issue: 122, P. 102649 - 102649

Published: Oct. 31, 2023

Cholangiocarcinomas (CCA) are a heterogeneous group of tumors that classified as intrahepatic, perihilar, or distal according to the anatomic location within biliary tract. Each CCA subtype is associated with distinct genomic alterations, including single nucleotide variants, copy number and chromosomal rearrangements gene fusions, each which can influence disease prognosis and/or treatment outcomes. Molecular profiling using next-generation sequencing (NGS) powerful technique for identifying unique variants carried by an individual tumor, facilitate their accurate diagnosis well promote optimal selection variant–matched targeted treatments. NGS particularly useful in patients because between one-third one-half these have alterations be drugs either approved clinical development. also provide information about evolution secondary resistance develop during therapy, thus assessment choice alternative Pathologists play critical role assessing viability biopsy samples NGS, advising treating clinicians whether performed available platforms should used optimize testing This review aims pathologists other healthcare professionals practical step-by-step guidance on use molecular CCA, respect tumor techniques, pre-analytic sample preparation, selecting appropriate panel, understanding interpreting results test.

Language: Английский

---

Heterogeneity of Cholangiocarcinoma Immune Biology

Cells, Journal Year: 2023, Volume and Issue: 12(6), P. 846 - 846

Published: March 8, 2023

Cholangiocarcinomas (CCAs) are aggressive tumors arising along the biliary tract epithelium, whose incidence and mortality increasing. CCAs highly desmoplastic cancers characterized by a dense tumor microenvironment (TME), in which each single component plays fundamental role shaping CCA initiation, progression resistance to therapies. The crosstalk between cancer cells TME can affect recruitment, infiltration differentiation of immune cells. According stage disease intra- inter-patient heterogeneity, may contribute either protumoral or antitumoral activities. Therefore, better understanding effect cell subtype open path new personalized therapeutic strategies for management CCA. In this review, we describe initiation progression, their with both cancer-associated fibroblasts (CAFs) cancer-stem-cell-like (CSC) niche.

Language: Английский

---

KIF14 promotes proliferation, lymphatic metastasis and chemoresistance through G3BP1/YBX1 mediated NF-κB pathway in cholangiocarcinoma

Oncogene, Journal Year: 2023, Volume and Issue: 42(17), P. 1392 - 1404

Published: March 15, 2023

Language: Английский

---

CircZNF215 promotes tumor growth and metastasis through inactivation of the PTEN/AKT pathway in intrahepatic cholangiocarcinoma

Journal of Experimental & Clinical Cancer Research, Journal Year: 2023, Volume and Issue: 42(1)

Published: May 18, 2023

Abstract Background Increasing evidence shows that circular RNAs (circRNAs), a novel class of noncoding RNAs, play crucial role in the development cancers, including intrahepatic cholangiocarcinoma (iCCA). Nevertheless, their functions and exact mechanisms iCCA progression metastasis are still unclear. Ipatasertib is highly selective inhibitor AKT inhibits tumor growth by blocking PI3K/AKT pathway. In addition, phosphatase tensin homolog (PTEN) can also inhibit activation pathway, but it not clear whether cZNF215-PRDX-PTEN axis plays antitumor activity ipatasertib. Methods We identified new circRNA (circZNF215, termed cZNF215) through high-throughput sequencing (circRNA-seq). RT‒qPCR, immunoblot assay, RNA pull-down immunoprecipitation (RIP) fluorescence situ hybridization assay (FISH) were used to investigate interaction cZNF215 with peroxiredoxin 1 (PRDX1). Coimmunoprecipitation (Co-IP) assays duolink proximity ligation (PLAs) conducted analyze effects on between PRDX1 PTEN. Finally, we tested potential ipatasertib vivo experiments. Results found expression was obviously upregulated tissues postoperative metastases correlated poor outcome patients iCCA. further revealed overexpression promoted cell vitro vivo, while knockdown had opposite effect. Mechanistic studies suggested competitively interacted PRDX1, which blocked association PTEN, subsequently leading oxidation-induced inactivation PTEN/AKT pathway finally contributing metastasis. Additionally, silencing cells enhance effect Conclusions Our study demonstrates facilitates regulating may serve as prognostic predictor

Language: Английский

---

Harnessing the supremacy of MEG3 LncRNA to defeat gastrointestinal malignancies

Pathology - Research and Practice, Journal Year: 2024, Volume and Issue: 256, P. 155223 - 155223

Published: Feb. 23, 2024

Language: Английский

---

Pathological, molecular, and clinical characteristics of cholangiocarcinoma: A comprehensive review

World Journal of Gastrointestinal Oncology, Journal Year: 2022, Volume and Issue: 14(3), P. 607 - 627

Published: March 8, 2022

Cholangiocarcinomas are a heterogeneous group of highly aggressive cancers that may arise anywhere within the biliary tree. There is wide geographical variation with regards to its incidence, and risk-factor associations which include liver fluke infection, primary sclerosing cholangitis, hepatolithiasis amongst others. These tumours classified into intrahepatic, perihilar distal based on their anatomical location. Morphologically, intrahepatic cholangiocarcinomas further sub-classified small large duct variants. Perihilar usually mucin-producing tubular adenocarcinomas. develop through multistep carcinogenesis preceded by dysplastic in situ lesions. While clinical characteristics management these have been extensively elucidated literature, ultra-structure tumour biology remain relatively unknown. This review focuses current knowledge pathological characteristics, molecular alterations cholangiocarcinoma, precursor lesions (including intraepithelial neoplasia, intraductal papillary neoplasms bile duct, tubulopapillary mucinous cystic neoplasm).

Language: Английский

---