Microbiological Research, Journal Year: 2024, Volume and Issue: 290, P. 127925 - 127925
Published: Oct. 17, 2024
Language: Английский
New Phytologist, Journal Year: 2024, Volume and Issue: unknown
Published: Nov. 4, 2024
Summary Ralstonia pseudosolanacearum , a plant pathogen responsible for bacterial wilt in numerous species, exhibits paradoxical growth the host by achieving high densities xylem sap, an environment traditionally considered nutrient‐poor. This study combined vitro experiments and mathematical modeling to elucidate population dynamics of R. within plants. To simulate environment, tomato xylem‐mimicking medium was developed. Then, model constructed using data employed density sap composition during infection. The accurately reproduced planta experimental observations, including depletion glutamine asparagine. Additionally, estimated minimal number bacteria required initiate infection, timing infection post‐inoculation, mortality rate at which putrescine is assimilated plant. findings demonstrate that can sustain densities, provides explanatory framework presence acetate, 3‐hydroxybutyrate infected give clues as role virulence pseudosolanacearum.
Language: Английский
Citations
2
Industrial Crops and Products, Journal Year: 2024, Volume and Issue: 220, P. 119269 - 119269
Published: July 26, 2024
Language: Английский
Citations
1
Molecular Plant Pathology, Journal Year: 2024, Volume and Issue: 25(8)
Published: Aug. 1, 2024
Ralstonia solanacearum species complex (RSSC) includes soilborne bacterial plant pathogens with worldwide distribution and wide host ranges. Virulence factors are regulated via four hierarchically organized cell-cell contact independent quorum-sensing (QS) signalling systems: the Phc, which uses as signals (R)-methyl 3-hydroxypalmitate [(R)-3-OH PAME] or 3-hydroxymyristate MAME], N-acyl homoserine lactone (AHL)-dependent RasI/R SolI/R systems, recently identified anthranilic acid-dependent system. The unique Phc QS system has been extensively studied; however, role of two AHL systems only addressed. In this microreview, we present discuss current data in RSSC. We also frequency these RSSC, possible ecological roles evolutive implications. hierarchical networks emphasizes crucial virulence
Language: Английский
Citations
1
PLoS Biology, Journal Year: 2024, Volume and Issue: 22(9), P. e3002792 - e3002792
Published: Sept. 20, 2024
Adaptation is usually explained by beneficial genetic mutations that are transmitted from parents to offspring and become fixed in the adapted population. However, mutation analysis alone not sufficient fully explain adaptive processes, several studies report existence of nongenetic (or epigenetic) inheritance can enable adaptation new environments. In present work, we tested hypothesis role DNA methylation, a form epigenetic modification, plant pathogen Ralstonia pseudosolanacearum host during experimental evolution. Using SMRT-seq technology, analyzed methylomes 31 experimentally evolved clones obtained after serial passages on 5 different species 300 generations. Comparison with methylome ancestral clone revealed list 50 differential methylated sites (DMSs) at GTWWAC motif. Gene expression 39 genes targeted these DMSs limited correlation between methylation corresponding genes. Only 1 gene showed correlation, RSp0338 encoding EpsR regulator protein. The MSRE-qPCR used as an alternative approach for analysis, also found 2 upstream RSp0338. site-directed mutagenesis, demonstrated contribution adaptation. As appeared very early evolution, hypothesize such fast changes allow rapid stem environment. addition, change remains stable least 100 generations outside thus contribute long-term plant. To our knowledge, this first study showing direct link bacterial variation
Language: Английский
Citations
1
Microbiological Research, Journal Year: 2024, Volume and Issue: 290, P. 127925 - 127925
Published: Oct. 17, 2024
Language: Английский
Citations
1