The structure and function of the global citrus rhizosphere microbiome

Nature Communications, Journal Year: 2018, Volume and Issue: 9(1)

Published: Nov. 14, 2018

Citrus is a globally important, perennial fruit crop whose rhizosphere microbiome thought to play an important role in promoting citrus growth and health. Here, we report comprehensive analysis of the structural functional composition microbiome. We use both amplicon deep shotgun metagenomic sequencing bulk soil samples collected across distinct biogeographical regions from six continents. Predominant taxa include Proteobacteria, Actinobacteria, Acidobacteria Bacteroidetes. The core comprises Pseudomonas, Agrobacterium, Cupriavidus, Bradyrhizobium, Rhizobium, Mesorhizobium, Burkholderia, Cellvibrio, Sphingomonas, Variovorax Paraburkholderia, some which are potential plant beneficial microbes. also identify over-represented microbial traits mediating plant-microbe microbe-microbe interactions, nutrition acquisition promotion rhizosphere. results provide valuable information guide isolation culturing and, potentially, harness power improve production

Language: Английский

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Antibiotic Resistance in Plant-Pathogenic Bacteria

Annual Review of Phytopathology, Journal Year: 2018, Volume and Issue: 56(1), P. 161 - 180

Published: June 1, 2018

Antibiotics have been used for the management of relatively few bacterial plant diseases and are largely restricted to high-value fruit crops because expense involved. Antibiotic resistance in plant-pathogenic bacteria has become a problem pathosystems where these antibiotics many years. Where genetic basis examined, antibiotic pathogens most often evolved through acquisition determinant via horizontal gene transfer. For example, strAB streptomycin-resistance genes occur Erwinia amylovora, Pseudomonas syringae, Xanthomonas campestris, presumably acquired from nonpathogenic epiphytic colocated on hosts under selection. We currently lack knowledge effect microbiome commensal organisms potential evolve resistance. Such is critical development robust strategies ensure safe effective continued use critically important diseases.

Language: Английский

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Mechanistic insights into host adaptation, virulence and epidemiology of the phytopathogenXanthomonas

FEMS Microbiology Reviews, Journal Year: 2019, Volume and Issue: 44(1), P. 1 - 32

Published: Oct. 1, 2019

ABSTRACT Xanthomonas is a well-studied genus of bacterial plant pathogens whose members cause variety diseases in economically important crops worldwide. Genomic and functional studies these phytopathogens have provided significant understanding microbial-host interactions, virulence host adaptation mechanisms including microbial ecology epidemiology. In addition, several strains are as producers the extracellular polysaccharide, xanthan, used food pharmaceutical industries. This polymer has also been implicated phases disease cycle. this review, we summarise current knowledge on infection strategies regulatory networks controlling from species discuss novel opportunities that body work for control health.

Language: Английский

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An effector from the Huanglongbing-associated pathogen targets citrus proteases

Nature Communications, Journal Year: 2018, Volume and Issue: 9(1)

Published: April 24, 2018

The citrus industry is facing an unprecedented challenge from Huanglongbing (HLB). All cultivars can be affected by the HLB-associated bacterium 'Candidatus Liberibacter asiaticus' (CLas) and there no known resistance. Insight into HLB pathogenesis urgently needed in order to develop effective management strategies. Here, we use Sec-delivered effector 1 (SDE1), which conserved all CLas isolates, as a molecular probe understand virulence. We show that SDE1 directly interacts with papain-like cysteine proteases (PLCPs) inhibits protease activity. PLCPs are defense-inducible exhibit increased protein accumulation CLas-infected trees, suggesting role defense responses. analyzed PLCP activity field samples, revealing specific members increase abundance but remain unchanged during infection. SDE1-expressing transgenic also reduced These data demonstrate PLCPs, immune-related enhance responses plants.

Language: Английский

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CRISPR‐LbCas12a‐mediated modification of citrus

Plant Biotechnology Journal, Journal Year: 2019, Volume and Issue: 17(10), P. 1928 - 1937

Published: March 25, 2019

Summary Recently, CRISPR ‐Cas12a (Cpf1) from Prevotella and Francisella was engineered to modify plant genomes. In this report, we employed ‐LbCas12a (LbCpf1), which is derived Lachnospiraceae bacterium ND 2006 , edit a citrus genome for the first time. First, LbCas12a used Cs PDS gene successfully in Duncan grapefruit via Xcc‐facilitated agroinfiltration. Next, driven by either 35S or Yao promoter PthA4 effector binding elements ( EBE P thA4 ‐Cs LOBP ) of LOB 1 . A single cr RNA selected target conserved region both Type I II s, since protospacer adjacent motif TTTV allows act on these two types canker susceptibility gene, it induced corresponding pathogenicity factor Xanthomonas citri total seven 35S‐LbCas12a‐transformed plants were generated, they designated as #D 35 s1 s7, ten Yao‐LbCas12a‐transformed created yao 10. LbCas12a‐directed modifications observed three (#D s1, s4 s7). However, no LbCas12a‐mediated indels plants. Notably, transgenic line s4, contains highest mutation rate, alleviates XccΔpthA4: dCsLOB 1.4 infection. Finally, potential off‐targets observed. Therefore, can readily be powerful tool editing.

Language: Английский

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Citrus disease detection and classification using end-to-end anchor-based deep learning model

Applied Intelligence, Journal Year: 2021, Volume and Issue: 52(1), P. 927 - 938

Published: May 13, 2021

Language: Английский

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Citrus Huanglongbing is a pathogen-triggered immune disease that can be mitigated with antioxidants and gibberellin

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Jan. 26, 2022

Abstract Huanglongbing (HLB) is a devastating disease of citrus, caused by the phloem-colonizing bacterium Candidatus Liberibacter asiaticus (CLas). Here, we present evidence that HLB an immune-mediated disease. We show CLas infection Citrus sinensis stimulates systemic and chronic immune responses in phloem tissue, including callose deposition, production reactive oxygen species (ROS) such as H 2 O , induction immunity-related genes. The also upregulates genes encoding ROS-producing NADPH oxidases, downregulates antioxidant enzyme genes, supporting causes oxidative stress. CLas-triggered ROS localizes phloem-enriched bark tissue followed cell death companion sieve element cells. Inhibition levels CLas-positive stems oxidase inhibitor diphenyleneiodonium (DPI) indicates oxidases contribute to production. To investigate potential treatments, addition growth hormone gibberellin (known have immunoregulatory activities) -scavenging enzymes oxidases. Furthermore, foliar spray HLB-affected citrus with or antioxidants (uric acid, rutin) reduces concentrations tissues symptoms. Thus, our results indicate can be mitigated gibberellin.

Language: Английский

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The Citrus Huanglongbing Crisis and Potential Solutions

Molecular Plant, Journal Year: 2019, Volume and Issue: 12(5), P. 607 - 609

Published: April 1, 2019

Language: Английский

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Plant Immune Mechanisms: From Reductionistic to Holistic Points of View

Molecular Plant, Journal Year: 2020, Volume and Issue: 13(10), P. 1358 - 1378

Published: Sept. 8, 2020

After three decades of the amazing progress made on molecular studies plant-microbe interactions (MPMI), we have begun to ask ourselves "what are major questions still remaining?" as if puzzle has only a few pieces missing. Such an exercise ultimately led realization that many more than answers. Therefore, it would be impossible task for us project coherent "big picture" MPMI field in single review. Instead, provide our opinions where like go research invitation community join this exploration new frontiers.

Language: Английский

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Challenges for Managing Candidatus Liberibacter spp. (Huanglongbing Disease Pathogen): Current Control Measures and Future Directions

Phytopathology, Journal Year: 2017, Volume and Issue: 108(4), P. 424 - 435

Published: Oct. 9, 2017

Huanglongbing (HLB; “citrus greening” disease) has caused significant damages to the global citrus industry as it become well established in leading citrus-producing regions and continues spread worldwide. Insecticidal control been a critical component of HLB disease management, there is direct relationship between vector Candidatus Liberibacter spp. (i.e., pathogen) titer HLB-infected trees. In recent years, have substantial efforts develop practical strategies for specifically managing Ca. spp.; however, literature review on outcomes such attempts still lacking. This work summarizes greenhouse field studies that documented effects implications chemical-based treatments applications broad-spectrum antibiotics, small molecule compounds) nonchemical measures plant-beneficial compounds, inorganic fertilizers, biological control, thermotherapy) phytopathogen control. The ongoing challenges associated with mitigating populations at field-scale, seasonality symptoms, limitations therapeutics contact planta, adverse impacts microbiota, potential public ecosystem health, are also discussed.

Language: Английский

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