Store-Operated CRAC Channels Regulate Gene Expression and Proliferation in Neural Progenitor Cells DOI Open Access
A. Somasundaram,

Andrew Shum,

Helen J. McBride

et al.

Journal of Neuroscience, Journal Year: 2014, Volume and Issue: 34(27), P. 9107 - 9123

Published: July 2, 2014

Calcium signals regulate many critical processes during vertebrate brain development including neurogenesis, neurotransmitter specification, and axonal outgrowth. However, the identity of ion channels mediating Ca2+ signaling in developing nervous system is not well defined. Here, we report that embryonic adult mouse neural stem/progenitor cells (NSCs/NPCs) exhibit store-operated entry (SOCE) mediated by release-activated (CRAC) channels. SOCE NPCs was blocked CRAC channel inhibitors La3+, BTP2, 2-APB Western blots revealed presence canonical proteins STIM1 Orai1. Knock down or Orai1 significantly diminished NPCs, lost from transgenic mice lacking knock-in expressing loss-of-function mutant, R93W. Therefore, make essential contributions to NPCs. activated epidermal growth factor acetylcholine, latter occurring through muscarinic receptors. Activation stimulated gene transcription calcineurin/NFAT (nuclear T cells) a mechanism consistent with local microdomains near Importantly, suppression deletion expression attenuated proliferation cultured as neurospheres and, in vivo, subventricular zone mice. These findings show serve major route key effector functions proliferation, indicating are important regulators mammalian neurogenesis.

Language: Английский

Minding the calcium store: Ryanodine receptor activation as a convergent mechanism of PCB toxicity DOI
Isaac N. Pessah,

Gennady Cherednichenko,

Pamela J. Lein

et al.

Pharmacology & Therapeutics, Journal Year: 2009, Volume and Issue: 125(2), P. 260 - 285

Published: Nov. 18, 2009

Language: Английский

Citations

228
CMIP and ATP2C2 Modulate Phonological Short-Term Memory in Language Impairment DOI Creative Commons
Dianne F. Newbury, Laura Winchester, Laura Addis

et al.

The American Journal of Human Genetics, Journal Year: 2009, Volume and Issue: 85(2), P. 264 - 272

Published: July 31, 2009

Language: Английский

Citations

190
Mutations in the calcium-related gene IL1RAPL1 are associated with autism DOI Open Access
Amélie Piton, Jacques L. Michaud, Boon‐Peng Hoh

et al.

Human Molecular Genetics, Journal Year: 2008, Volume and Issue: 17(24), P. 3965 - 3974

Published: Sept. 4, 2008

In a systematic sequencing screen of synaptic genes on the X chromosome, we have identified an autistic female without mental retardation (MR) who carries de novo frameshift Ile367SerfsX6 mutation in Interleukin-1 Receptor Accessory Protein-Like 1 (IL1RAPL1), gene implicated calcium-regulated vesicle release and dendrite differentiation. We showed that function resulting truncated IL1RAPL1 protein is severely altered hippocampal neurons, by measuring its effect neurite outgrowth activity. also sequenced coding region close related member IL1RAPL2 NCS-1/FREQ, which physically interacts with IL1RAPL1, cohort subjects autism. The screening failed to identify non-synonymous variant IL1RAPL2, whereas rare missense (R102Q) NCS-1/FREQ was one patient. Furthermore, comparative genomic hybridization large intragenic deletion exons 3–7 three brothers autism and/or MR. This causes introduction premature stop codon, Ala28GlufsX15, at very beginning protein. All together, our results indicate mutations cause spectrum neurological impairments ranging from MR high functioning

Language: Английский

Citations

187
Connecting the ear to the brain: Molecular mechanisms of auditory circuit assembly DOI

Jessica M. Appler,

Lisa V. Goodrich

Progress in Neurobiology, Journal Year: 2011, Volume and Issue: 93(4), P. 488 - 508

Published: Jan. 12, 2011

Language: Английский

Citations

179
Uncoupling Dendrite Growth and Patterning: Single-Cell Knockout Analysis of NMDA Receptor 2B DOI Creative Commons

J. Sebastian Espinosa,

Damian G. Wheeler,

Richard W. Tsien

et al.

Neuron, Journal Year: 2009, Volume and Issue: 62(2), P. 205 - 217

Published: April 1, 2009

Language: Английский

Citations

171
Growth cone chemotaxis DOI

Duncan Mortimer,

Thomas Fothergill,

Zac Pujic

et al.

Trends in Neurosciences, Journal Year: 2008, Volume and Issue: 31(2), P. 90 - 98

Published: Jan. 17, 2008

Language: Английский

Citations

164
Wnt5a Induces Simultaneous Cortical Axon Outgrowth and Repulsive Axon Guidance through Distinct Signaling Mechanisms DOI Creative Commons
Li Li, B. Ian Hutchins,

Katherine Kalil

et al.

Journal of Neuroscience, Journal Year: 2009, Volume and Issue: 29(18), P. 5873 - 5883

Published: May 6, 2009

Wnts are morphogens that also function as axon guidance molecules. In vivo Wnt5a gradients via Ryk receptors were found to repel cortical axons into developing callosal and corticospinal pathways. Here, using dissociated cultures, we bath-applied increased outgrowth. turning assays, simultaneously outgrowth induced repulsive turning, a potential mechanism for propelling in . We is mediated by Ryk, whereas repulsion requires both Frizzled receptors. Both mediate Wnt-evoked fluctuations intracellular calcium, which required Wnt5a. However, involves calcium release from stores through IP3 well influx TRP channels, channels without involvement of These results reveal distinct signaling mechanisms underlying Wnt5a-induced guidance.

Language: Английский

Citations

163
Neurite Branch Retraction Is Caused by a Threshold-Dependent Mechanical Impact DOI Creative Commons
Kristian Franze,

Jens C. Gerdelmann,

Michael Weick

et al.

Biophysical Journal, Journal Year: 2009, Volume and Issue: 97(7), P. 1883 - 1890

Published: Oct. 1, 2009

Language: Английский

Citations

160
The biophysics of neuronal growth DOI
Kristian Franze, Jochen Guck

Reports on Progress in Physics, Journal Year: 2010, Volume and Issue: 73(9), P. 094601 - 094601

Published: Aug. 18, 2010

For a long time, neuroscience has focused on biochemical, molecular biological and electrophysiological aspects of neuronal physiology pathology. However, there is growing body evidence indicating the importance physical stimuli for growth development. In this review we briefly summarize historical background neurobiophysics give an overview over current understanding from physics perspective. We show how biophysics so far contributed to better discuss inconsistencies. Finally, speculate may contribute successful treatment lesions central nervous system, which have been considered incurable until very recently.

Language: Английский

Citations

153
Integrative Mechanisms of Oriented Neuronal Migration in the Developing Brain DOI
Irina Y. Evsyukova,

Charlotte Plestant,

E.S. Anton

et al.

Annual Review of Cell and Developmental Biology, Journal Year: 2013, Volume and Issue: 29(1), P. 299 - 353

Published: Aug. 12, 2013

The emergence of functional neuronal connectivity in the developing cerebral cortex depends on migration. This process enables appropriate positioning neurons and identity so that correct patterns synaptic between right types numbers can emerge. Delineating complexities migration is critical to our understanding normal cortical formation neurodevelopmental disorders resulting from defects. For most part, integrated cell biological basis complex behavior oriented within mammalian remains an enigma. review aims analyze integrative mechanisms enable sense environmental guidance cues translate them into toward defined areas cortex. We discuss how signals emanating different domains get control distinct aspects migratory coordinate their activities produce functionally laminar organization.

Language: Английский

Citations

141