A Novel Flexible Electrochemical Molecular Imprinted Sensor for the Determination of GABA in Serum of Depressed Mice

Journal of The Electrochemical Society, Journal Year: 2023, Volume and Issue: 170(1), P. 017504 - 017504

Published: Jan. 1, 2023

The content of γ -aminobutyric acid (GABA) in human serum has been proved to be closely related depression, so the rapid, sensitive, highly selective and accurate determination GABA is great significance for objective diagnosis depression. In this study, a molecularly imprinted electrochemical sensor based on high-performance magnetically functionalized reduced graphene oxide (MRGO) was constructed sensitive detection depressed mice. Using MRGO modified flexible electrode (ITO-PET), 2,4-dinitrofluorobenzene derivative as template pyrrole with self-polymerization effect functional monomer cross-linking agent, MIP/MRGO/ITO-PET successfully by simple electropolymerization stirring elution. Under optimal construction process, realized range 250 pM ∼ 100 μ M, limit low 1.25 × 10 −10 M (S/N = 3). simultaneous possession high sensitivity selectivity, good stability, repeatability reproducibility enables applicable mice serum. results showed that expected provide basis depression clinic.

Language: Английский

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Biocompatibility of Poly-Lactic Acid/Nanohydroxyapatite/Graphene Nanocomposites for Load Bearing Bone Implants

Journal of The Electrochemical Society, Journal Year: 2023, Volume and Issue: 170(2), P. 027502 - 027502

Published: Jan. 31, 2023

In this study, poly-lactic acid (PLA), nanohydroxyapatite (NHA), and graphene nanoplatelets (GNP) were blended to develop a nanocomposite material suitable for load-bearing bone implants with the potential strain-sensing applications. The tensile properties impact strength of PLA-NHA analyzed, as these are crucial biomechanical It was found that decreased by 7.9% (0.05 wt% GNP) 25.7% (0.1 increasing GNP loading compared 0.01 GNP. Besides, biocompatibility nanocomposites (PLA-NHA, PLA-mNHA, PLA-mNHA-GNP) evaluated through in-vitro analysis attaching MG63 cells observing their proliferation differentiation over 7 21 days incubation. prepared determined based on ability attach cells, thus allowing proliferate enhance differentiate. Results showed PLA-5wt%NHA provided better cell spreading pure PLA, while PLA-5wt%mNHA had highest rate proliferation. further confirmed field emission scanning electron microscope (FESEM) imaging, MTT assays, alkaline phosphatase (ALP) assays.

Language: Английский

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Effect of Solution Aging on Temperature Sensitivity of CNT/PEDOT:PSS

ECS Journal of Solid State Science and Technology, Journal Year: 2023, Volume and Issue: 12(2), P. 027001 - 027001

Published: Jan. 23, 2023

This study fabricated temperature sensors with a mixture of carbon nanotube (CNT) and poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT:PSS). CNT/PEDOT:PSS solutions three different ratios—1/5, 1/9, 1/13—prepared aged for 0, 3, 6 days. The sensor was by drop-casting the solution onto flexible polydimethylsiloxane (PDMS) substrate. As aging time ratios 1/5, 1/13 increased to days, coefficient resistance (TCR) decreased from −1.97, −1.99, −2.15 (%/°C) −2.80, −2.61, −2.51 (i.e. sensitivity increased) index (TI) also 2242.5, 2249.1, 2503.8 (K) 3530.1, 3085.7, 3002.5 (K), respectively. its Seebeck 52.4 114.5 μ V K −1 . These results show that significantly via dedoping PEDOT:PSS. serves as facile method improve composite.

Language: Английский

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Flexible Paper-Based Immunosensor for the Detection of Specific Cancer-Derived Exosomes

Journal of The Electrochemical Society, Journal Year: 2022, Volume and Issue: 169(12), P. 127514 - 127514

Published: Dec. 1, 2022

This study aims at an electrochemical paper-based device for the selective and sensitive detection of exosomes as economic flexible platform. Using a laser cutter, three-electrode platform was patterned on hydrophobic paper, then synthetic carbon silver ink were screen-printed paper. Anti-human CD9 antibody covalently attached to surface electrode enriched with graphene oxide aerogel/CoFe 2 O 4 @chitosan nanocomposite. The immobilization, incubation binding time capture investigated effective parameters. Under optimum conditions, differential pulse voltammetry (DPV) used establish calibration plot that covered 500 5 × 10 6 exosoms μ l −1 limit 100 exosome . method implemented successfully in serum sample. As results show, fabricated biosensor is capable detecting early diagnosis cancers.

Language: Английский

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Novel Electrochemical Sensor Application for Dopamine and Preparation of N-rGO Micro-regionally Constrained WS₂ Nanocomposite

Journal of The Electrochemical Society, Journal Year: 2022, Volume and Issue: 169(12), P. 127518 - 127518

Published: Dec. 1, 2022

Dopamine is an important neurotransmitter for organisms and affects their function, so monitoring detecting dopamine levels are essential. Sensitivity selectivity of electrochemical sensors the detection usually rely on hybrid nanocomposites. In this context, we designed a micro-regionally restricted nanocomposite by modulating micro-nano structure. Using functional groups nitrogen-doped reduced graphene oxide (N-rGO) to restrict random growth tungsten disulfide nanosheets, obtained 2D layered material with bidirectional structural support achieve high specific surface activity application in robust novel sensors. A facile sensor was fabricated modifying screen-printed carbon electrode nanocomposite. The exhibits excellent performance wide range 1 μ mol l −1 –100 limit 0.2 sensitivity 0.56 . optimized shows good selectivity, satisfactory long-term stability reproducibility towards DA separation peak potential 142 mV uric acid. addition, prepared provides favorable recovery human serum artificial cerebrospinal solution (aSCF), indicating great potentials biological sensing applications.

Language: Английский

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