Human skeletal muscle possesses both reversible proteomic signatures and a retained proteomic memory after repeated resistance training DOI Creative Commons
Juha J. Hulmi, Eeli J. Halonen, Adam P. Sharples

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 21, 2024

Abstract Investigating repeated resistance training separated by a break enables exploration of the potential for proteomic memory (RT)-induced skeletal muscle growth. Our aim was to examine proteome response 10-week RT (RT1) followed cessation (i.e. detraining, DT), and finally, retraining (RT2). Thirty healthy, untrained participants conducted either periodic (RT1-DT-RT2, n=17) or no-training control period (n=13) 20 weeks (n=11). included twice-weekly supervised whole-body sessions, resting vastus lateralis biopsies were obtained every ten proteomics analysis using high-end DIA-PASEF’s mass spectrometry. The first altered 150 proteins (93% increased) involved in e.g. energy metabolism protein processing compared with minor changes during period. adaptations similar after second baseline demonstrating reproducibility RT. Many induced RT1 reversed towards detraining increased again retraining. These reversible especially aerobic metabolism. Interestingly, several remain elevated including carbonyl reductase 1 (CBR1) contraction, cytoskeleton calcium-binding. Amongst latter, calcium-activated protease calpain-2 (CAPN2) has been recently identified as an epigenetic gene. We show that evokes retained levels even 2.5 months no training. This is study demonstrate training-induced growth human muscle. Key points Repeated humans detraining) identification temporal signatures over training, periods well studying Muscle when baseline, earlier Several calcium-binding such (CAPN2), Human experiences following persisting two Figure investigated DT RT2. However, RT-induced DT, many contraction shows demonstrates Created BioRender.com.

Language: Английский

Enhancing muscle and brain resilience: The role of prehabilitative exercise in mitigating disuse effects DOI Open Access
Casper Soendenbroe, Carl‐Johan Boraxbekk, Abigail L. Mackey

et al.

The Journal of Physiology, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 6, 2025

Abstract Short‐term disuse leads to rapid declines in muscle mass and strength. These are driven by changes at all levels of the neuromuscular system; brain, spinal cord skeletal muscle. In addition neural input from central peripheral nervous systems muscle, molecular factors originating can be transported system. interactions highlight interconnected nature system during exercise disuse, form basis for this review. Although it is well known that physical activity confers a myriad health benefits, recent interest targeted before periods or immobility, termed prehabilitation, has emerged. Clinical studies within multiple medical specialities suggest positive effects prehabilitative on preserving function, reducing adverse outcomes shortening length hospital stay. Yet, available few heterogeneous, underlying protective mechanisms remain elusive. review, we examine ramifications across explore how prehabilitation may counteract these effects. We summarize into three primary categories: (1) enhancing pre‐disuse capacity; (2) establishing memory; (3) fostering structural adaptations both brain. image

Language: Английский

Citations

1
Molecular aspects of the exercise response and training adaptation in skeletal muscle DOI Creative Commons
Regula Furrer, Christoph Handschin

Free Radical Biology and Medicine, Journal Year: 2024, Volume and Issue: 223, P. 53 - 68

Published: July 24, 2024

Skeletal muscle plasticity enables an enormous potential to adapt various internal and external stimuli perturbations. Most notably, changes in contractile activity evoke a massive remodeling of biochemical, metabolic force-generating properties. In recent years, large number signals, sensors, regulators effectors have been implicated these adaptive processes. Nevertheless, our understanding the molecular underpinnings training adaptation remains rudimentary. Specifically, mechanisms that underlie signal integration, output coordination, functional redundancy other complex traits are unknown. fact, it is even unclear how stimulus-dependent specification brought about endurance or resistance exercise. this review, we will provide overview on events describe acute perturbations single exercise bouts. Furthermore, insights into principles long-term adaptation. Finally, current gaps knowledge be identified, strategies for multi-omic –cellular analyses skeletal engaged individual, bouts chronic discussed.

Language: Английский

Citations

7
Mitigating disuse‐induced skeletal muscle atrophy in ageing: Resistance exercise as a critical countermeasure DOI Creative Commons
James McKendry, Giulia Coletta, Everson Araújo Nunes

et al.

Experimental Physiology, Journal Year: 2024, Volume and Issue: 109(10), P. 1650 - 1662

Published: Aug. 6, 2024

The gradual deterioration of physiological systems with ageing makes it difficult to maintain skeletal muscle mass (sarcopenia), at least partly due the presence 'anabolic resistance', resulting in loss. Sarcopenia can be transiently but markedly accelerated through periods disuse-induced (i.e., unloading) atrophy reduced physical activity, sickness, immobilisation or hospitalisation. Periods disuse are detrimental older adults' overall quality life and substantially increase their risk falls, social dependence, early mortality. Disuse events induce various mechanisms, including anabolic resistance, inflammation, disturbed proteostasis mitochondrial dysfunction, all which tip scales favour a negative net protein balance subsequent Concerningly, recovery from is more for adults than younger counterparts. Resistance training (RT) potent stimulus that robustly stimulate synthesis mitigate losses when implemented before, during following unloading. RT may take form traditional weightlifting-focused RT, bodyweight lower- higher-load RT. When combined sufficient dietary protein, accelerate event, frailty improve mobility; however, few regularly participate A feasible practical approach improving accessibility acceptability use resistance bands. Moving forward, must prescribed consequences atrophy.

Language: Английский

Citations

6
Metabolomic and proteomic applications to exercise biomedicine DOI Creative Commons
Daniel J. Wilkinson, Hannah Crossland, Philip J. Atherton

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 1(1), P. 9 - 22

Published: March 20, 2024

'OMICs encapsulates study of scaled data acquisition, at the levels DNA, RNA, protein, and metabolite species. The broad objectives OMICs in biomedical exercise research are multifarious, but commonly relate to biomarker development understanding features adaptation health, ageing metabolic diseases.

Language: Английский

Citations

5
Epigenetics of Genes Preferentially Expressed in Dissimilar Cell Populations: Myoblasts and Cerebellum DOI Creative Commons
Melanie Ehrlich,

Kenneth C. Ehrlich,

Michelle Lacey

et al.

Epigenomes, Journal Year: 2024, Volume and Issue: 8(1), P. 4 - 4

Published: Jan. 26, 2024

While studying myoblast methylomes and transcriptomes, we found that CDH15 had a remarkable preference for expression in both myoblasts cerebellum. To understand how widespread such relationship was its epigenetic biological correlates, systematically looked genes with similar transcription profiles analyzed their DNA methylation chromatin state accessibility many different cell populations. Twenty were expressed preferentially cerebellum (Myob/Cbl genes). Some shared hypo- or hypermethylated regions Particularly striking ZNF556, whose promoter is hypomethylated expressing cells but highly methylated the populations do not express gene. In reporter gene assays, demonstrated promoter’s activity sensitive. The atypical epigenetics of ZNF556 may have originated from hypomethylation selective activation sperm progenitors oocytes. Five Myob/Cbl (KCNJ12, ST8SIA5, ZIC1, VAX2, EN2) much higher RNA levels than displayed myoblast-specific hypermethylation upstream and/or downstream promoters downmodulate expression. Differential associated alternative usage MCF2L, DOK7, CNPY1, ANK1. PAX3, LBX1, EN2, VAX2 encode sequence-specific factors, which likely help drive specificity other genes. This study extends our understanding epigenetic/transcription associations related to differentiation elucidate relationships between signatures muscular dystrophies cerebellar-linked neuropathologies.

Language: Английский

Citations

4
Number of physiotherapy sessions in work-related absenteeism due to musculoskeletal disorders, by gender, age and occupation. A retrospective cohort study DOI Creative Commons
Mònica Rodríguez-Bagó, Elena Ronda,

Emili Molina-Vega

et al.

Work, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 9, 2025

Background The number of physiotherapy sessions needed to treat musculoskeletal conditions varies in the literature; age and gender may partly explain discordant reports. However, no research has analysed whether occupation influence this outcome working population. Objectives To assess performed for low back pain (LBP), cervicalgia (CG), whiplash syndrome (WS) workers on sickness absence, according gender, age, occupation. Methods In retrospective cohort study, variable was recover from LBP, CG, WS. Explanatory variables were sex, occupation, year when ended, treatment centre. adjusted median differences (MDa) calculated. Results Older (55–65 years) a 2.6 additional 3.0 more 3.6 Men underwent fewer than women (LBP CG: MDa −0.9 sessions; WS: −1.7 sessions). Compared crafts related trades workers, plant machine operators assemblers required LBP (MDa 0.7), as did service sales 0.7). CG WS, observed technicians associate professionals 1.3 1.7, respectively), 2.4 1.6). Clerical support also significantly CG. Conclusions WS work-related absence is different

Language: Английский

Citations

0
The Effects of 8-week Dance and Tai Chi Randomized Controlled Trial on Balance, Wellbeing and Moods Among Healthy Adults Aged 18 to 60: A Study Protocol DOI Creative Commons
Jie Lu, Jake K. Ngo, Tracey J. Devonport

et al.

Journal of Dance Medicine & Science, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 28, 2025

Purpose: The aim of the proposed randomized controlled study is to compare effects and efficacy a dance, Tai Chi waiting list control group intervention over an 8-week period on physical functions mental benefits among non-clinical adults aged between 18 60. Method: A double-blind, trial will be adopted evaluate dance compared with benefits. Participants randomly assigned 3 groups, Chi, Control group, using stratified random sampling based sex. Isometric Mid-thigh Pull (IMTP), Y-Balance test (YBT), Warwick-Edinburgh Mental Wellbeing Scale (WEMWBS), Brunel Mood (BRUMS) are utilized for collecting data. All measurements conducted by research assistants blinded participants’ allocation analysis carried out statistician parameters. Expected result: wellbeing parameters predicted increasing during learning phase have improvement in practising phase.

Language: Английский

Citations

0
Influencing Factors of Medical Exercise Therapy DOI

Sandro Wolfram,

Robin Bauer

Springer eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 239 - 247

Published: Jan. 1, 2025

Language: Английский

Citations

0
Human skeletal muscle possesses both reversible proteomic signatures and a retained proteomic memory after repeated resistance training DOI
Juha J. Hulmi, Eeli J. Halonen, Adam P. Sharples

et al.

The Journal of Physiology, Journal Year: 2025, Volume and Issue: unknown

Published: April 4, 2025

Investigating repeated resistance training (RT) separated by a break enables exploration of the potential for proteomic memory RT-induced skeletal muscle growth, i.e. retained protein adaptations from previous RT. Our aim was to examine proteome response 10-week RT (RT1) followed cessation (i.e. detraining, DT), and finally, retraining (RT2). Thirty healthy, untrained participants conducted either periodic (RT1-DT-RT2, n = 17) or no-training control period (n 13) 20 weeks 11). included twice-weekly supervised whole-body sessions, resting vastus lateralis biopsies were obtained every 10 proteomics analysis using high-end dia-PASEF's mass spectrometry. The first altered 150 proteins (93% increased) involved in, example, energy metabolism processing compared minor changes during period. similar after second baseline demonstrating reproducibility in Many induced RT1 reversed towards detraining increased again retraining. These reversible especially aerobic metabolism. Interestingly, several which remain elevated retained) including carbonyl reductase 1 (CBR1) contraction, cytoskeleton calcium binding. Among latter, calcium-activated protease calpain-2 (CAPN2) has been recently identified as an epigenetic gene. We show that evokes levels even 2.5 months no training, demonstrates training-induced growth human muscle. KEY POINTS: Repeated humans detraining) identification temporal signatures over periods, well studying training. Muscle earlier Several calcium-binding such (CAPN2), Human experiences following persisting 2 months, growth.

Language: Английский

Citations

0
Unlocking the power of muscle memory: Advanced techniques for post-traumatic rehabilitation and return to competitive sports DOI
Roberto Tedeschi

Journal of Musculoskeletal Surgery and Research, Journal Year: 2024, Volume and Issue: 9, P. 157 - 159

Published: Sept. 3, 2024

Language: Английский

Citations

3