Sall4 regulates downstream patterning genes during limb regeneration DOI Creative Commons

James R. Erickson,

S. E. Walker,

Claudia Marcela Arenas Gómez

et al.

Developmental Biology, Journal Year: 2024, Volume and Issue: 515, P. 151 - 159

Published: July 26, 2024

Many salamanders can completely regenerate a fully functional limb. Limb regeneration is carefully coordinated process involving several defined stages. One key event during the patterning of blastema to inform cells what they must differentiate into. Although it known that many genes involved in initial development limb are re-used regeneration, exact molecular circuitry this not understood. Several large-scale transcriptional profiling studies axolotl have identified transcription factors up-regulated after amputation. Sall4 factor has been play essential roles maintaining an undifferentiated state and also plays unique role development. Inactivation bud results defects anterior-posterior found be both Xenopus salamanders, but date function untested. We confirmed using qRT-PCR present skin within blastema. Using CRISPR technology we microinjected gRNAs specific for complexed with cas9 protein into specifically knockout only. This resulted defects, including missing digits, fusion digit elements, radius ulna. suggests may similar regulating specification anterior-proximal skeletal elements.

Language: Английский

Divergent stem cell mechanisms governing the primary body axis and appendage regeneration in the axolotl DOI Open Access
Liqun Wang, Song Li,

Chao Yi

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 11, 2025

SUMMARY Exploring the fundamental mechanisms of organ regeneration is crucial for advancing regenerative medicine. The axolotl tail represents a unique opportunity to study primary axis including segmented muscle, vertebrae and skin. During development, muscle stem cells (MuSCs) displayed expected specificity lineage. Tail amputation, however, induced expansion MuSC potential yielding clonal contribution connective tissue cartilage, pericytes, fibroblasts. This expanded was not observed during limb regeneration, cross-transplantation showed these differences in are intrinsic. ScRNA-Seq profiling revealed that MuSCs revert an embryonic mesoderm-like state. Through genetic manipulation involving over-expression constitutively active TGF-β receptors or Smad7 (antagonist signaling) MuSCs, we demonstrated levels signal determine fate outcome lineage respectively. Our findings illustrate difference between versus offers novel cell source axial skeletal tissues.

Language: Английский

Citations

0
Cellular Dedifferentiation. Revisiting Betty Hay’s Legacy. DOI

Alejandra Beltrán-Rivera,

José E. García‐Arrarás

Developmental Biology, Journal Year: 2025, Volume and Issue: unknown

Published: March 1, 2025

Language: Английский

Citations

0
Generation of human expandable limb-bud-like progenitors via chemically induced dedifferentiation DOI

Jialiang Zhu,

Xinxing Zhong,

Huanjing He

et al.

Cell stem cell, Journal Year: 2024, Volume and Issue: 31(12), P. 1732 - 1740.e6

Published: Oct. 22, 2024

Language: Английский

Citations

1
Sall4 regulates downstream patterning genes during limb regeneration DOI Creative Commons

James R. Erickson,

S. E. Walker,

Claudia Marcela Arenas Gómez

et al.

Developmental Biology, Journal Year: 2024, Volume and Issue: 515, P. 151 - 159

Published: July 26, 2024

Many salamanders can completely regenerate a fully functional limb. Limb regeneration is carefully coordinated process involving several defined stages. One key event during the patterning of blastema to inform cells what they must differentiate into. Although it known that many genes involved in initial development limb are re-used regeneration, exact molecular circuitry this not understood. Several large-scale transcriptional profiling studies axolotl have identified transcription factors up-regulated after amputation. Sall4 factor has been play essential roles maintaining an undifferentiated state and also plays unique role development. Inactivation bud results defects anterior-posterior found be both Xenopus salamanders, but date function untested. We confirmed using qRT-PCR present skin within blastema. Using CRISPR technology we microinjected gRNAs specific for complexed with cas9 protein into specifically knockout only. This resulted defects, including missing digits, fusion digit elements, radius ulna. suggests may similar regulating specification anterior-proximal skeletal elements.

Language: Английский

Citations

1