Enhanced Cell Proliferation, Migration, and Fibroblast Differentiation with Electrospun PCL–Zinc Scaffolds Coated with Fibroblast-Derived ECM DOI Creative Commons
Alexis Moody, Narayan Bhattarai

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Despite tremendous improvement in the development of tissue-regenerating materials, a promising solution that provides an optimal environment remains to be accomplished. Here, we report composite nanofiber biomaterial scaffold as closely mimics extracellular matrix (ECM) improve cell viability, proliferation, and migration. Initially, composites polycaprolactone (PCL) zinc (Zn) metal were fabricated by using electrospinning. The resulting PCL–Zn (PZ) nanofibers effectively guided growth NIH3T3 fibroblasts for 7 days, forming fibroblast sheet. PZ fibers decellularized remove autologous allogenic cellular antigens while leaving intact ECM with structural functional components. PCL–Zn–ECM (PZE) showcased natural bonded surface, providing bioactive element interconnected fibers. reseeding demonstrated scaffold's excellent capacity direct support proliferation. Furthermore, vitro cytotoxicity analysis morphological staining confer biocompatibility. PZE presents which these scaffolds can further used various regenerative medicine applications including wound healing.

Language: Английский

Enhanced Cell Proliferation, Migration, and Fibroblast Differentiation with Electrospun PCL–Zinc Scaffolds Coated with Fibroblast-Derived ECM DOI Creative Commons
Alexis Moody, Narayan Bhattarai

ACS Omega, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

Despite tremendous improvement in the development of tissue-regenerating materials, a promising solution that provides an optimal environment remains to be accomplished. Here, we report composite nanofiber biomaterial scaffold as closely mimics extracellular matrix (ECM) improve cell viability, proliferation, and migration. Initially, composites polycaprolactone (PCL) zinc (Zn) metal were fabricated by using electrospinning. The resulting PCL–Zn (PZ) nanofibers effectively guided growth NIH3T3 fibroblasts for 7 days, forming fibroblast sheet. PZ fibers decellularized remove autologous allogenic cellular antigens while leaving intact ECM with structural functional components. PCL–Zn–ECM (PZE) showcased natural bonded surface, providing bioactive element interconnected fibers. reseeding demonstrated scaffold's excellent capacity direct support proliferation. Furthermore, vitro cytotoxicity analysis morphological staining confer biocompatibility. PZE presents which these scaffolds can further used various regenerative medicine applications including wound healing.

Language: Английский

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