World Allergy Organization Journal, Journal Year: 2024, Volume and Issue: 17(12), P. 100995 - 100995
Published: Nov. 21, 2024
Language: Английский
International Journal of COPD, Journal Year: 2024, Volume and Issue: Volume 19, P. 555 - 565
Published: Feb. 1, 2024
Background: Data are scarce on respiratory infections during severe acute exacerbation of chronic obstructive pulmonary disease (COPD). This study aimed to investigate infection patterns in the intensive care unit (ICU) and identify variables associated with type patient outcome. Methods: A retrospective, single-centre cohort study. All patients admitted (2015– 2021) our ICU for COPD were included. Logistic multivariable regression analysis was performed predict factors assess association between Results: We included 473 patients: 288 (60.9%) had 139 (29.4%) required invasive mechanical ventilation. Eighty-nine (30.9%) viral, 81 (28.1%) bacterial, 34 (11.8%) mixed, 84 (29.2%) undocumented infections. Forty-seven (9.9%) died 67 (14.2%) hospital. Factors temperature (odds ratio [+1°C]=1.43, P= 0.008) blood neutrophils (1.07, 0.002). Male sex (2.21, 0.02) bacterial (1.06, 0.04). In a analysis, pneumonia (cause-specific hazard=1.75, 0.005), rate (1.17, P =0.04), arterial partial pressure carbon-dioxide (1.08, 0.04), lactate (1.14, need MV. Age (1.03, 0.03), immunodeficiency (1.96, 0.02), altered performance status (1.78, 0.002) hospital mortality. Conclusions: Respiratory infections, 39.9% which main cause COPD. Body single markers infection. Pneumonia ventilation but not mortality, as opposed age, immunodeficiency, status. Plain Language Summary: - investigates prevalence, characteristics, impact outcomes different types triggering exacerbations COPD.- Our retrospective critically ill found that exacerbation.- The (viral, or mixt) mortality.- Early identification infectious agent is crucial implementing effective therapy; however, outcomes. Keywords: pneumonia, infection, COPD, ventilation, mortality
Language: Английский
Citations
4
bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown
Published: April 16, 2025
Abstract Despite the lungs being essential for ventilation and aerobic exercise capacity, conventionally are not thought to adapt training. Endurance is key pulmonary rehabilitation programs, which also displays sex-specific differences in therapeutic efficacy. Given molecular underpinnings of lung adaptations endurance uncharacterized, we used a multi-omics approach study sex 6-month-old Fischer 344 rats response an 8 week progressive treadmill training protocol. This was accomplished by reannotating publicly accessible data from Molecular Transducers Physical Activity Consortium (MoTrPAC) integrating newly-analyzed acetylome assess multi-omic sedentary progressively trained states. Female displayed enrichment immune-related features pathways at transcriptome proteome level that were maintained with Conversely, male rat there overall decrease immune following weeks Sexually conserved responses included increased transcriptomic related type I alveoli proteomic cilia, decreased mitochondrial protein acetylation. In both sexes, known be enriched diseases attenuated Together our findings provide novel insight into specific may offer translational disease pathogenesis treatment.
Language: Английский
Citations
0
Respiratory Research, Journal Year: 2024, Volume and Issue: 25(1)
Published: May 10, 2024
The impact of cigarette smoke (CS) on lung diseases and the role microbiome dysbiosis in chronic obstructive pulmonary disease (COPD) have been previously reported; however, relationships remain unclear. Our research examined effects 20-week exposure intestinal microbiomes C57BL/6JNarl mice, alongside a comparison with COPD patients' data from public dataset. study found that CS significantly decreased forced vital capacity (FVC), thickened airway walls, induced emphysema. Increased damage was observed along higher keratinocyte chemoattractant (KC) levels by exposure. Lung analysis revealed rise Actinobacteriota, while showed significant diversity changes, indicating dysbiosis. Principal coordinate highlighted distinct compositions between control CS-exposed groups. In microbiome, notable decreases Patescibacteria, Campilobacterota, Defferibacterota, Desulfobacterota were observed. We also identified correlations function both microbiomes. interleukins, interferon-ɣ, KC, 8-isoprostane linked to Notably, patterns mice similar those patients, particularly Global Initiative for Chronic Obstructive Disease (GOLD) stage 4 patients. This suggests systemic summary, induces microbiomes, correlating decline injury. These results align changes underscoring important smoke-related diseases.
Language: Английский
Citations
3
Journal of Inflammation Research, Journal Year: 2023, Volume and Issue: Volume 16, P. 5647 - 5665
Published: Nov. 1, 2023
This study aims to investigate the association between immune cells and development of COPD, while providing a new method for diagnosis COPD according changes in microenvironment.In this study, "CIBERSORT" algorithm was used estimate tissue infiltration 22 types GSE20257 GSE10006. The "limma" package differentially expressed analysis. key modules associated with vital were identified using WGCNA. GO KEGG enrichment analysis revealed biological functions candidate genes. Ultimately, novel diagnostic prediction model constructed via machine learning methods multivariate logistic regression based on GSE20257. Furthermore, we examined stability one internal test set (GSE10006), three external sets (GSE8545, GSE57148 GSE76925), single-cell transcriptome dataset (GSE167295), macrophages (THP-M cells) lung from patients.M0 (AUC > 0.7 GSE10006) considered as most important through exploring microenvironment landscapes patients healthy controls. genes GSE10006 divided into six five WGCNA, respectively. green module (cor = 0.41, P < 0.001) brown 0.67, highly correlated M0 selected modules. Forty-one intersected obtained two primarily involved regulation cytokine production, innate response, specific granule, phagosome, lysosome, ferroptosis, other processes. On basis genetic markers further characterized "Boruta" "LASSO" comprising CLEC5A, FTL SLC2A3 constructed, which could accurately distinguish controls multiple datasets. training has an AUC 0.916. AUCs 0.873, 0.932, 0.675 0.688, Single-cell sequencing suggested that patients. expressions up-regulated THP-M patients.According variations patients, validated macrophage M0-associated satisfactory predictive value. are expected be promising targets immunotherapy COPD.
Language: Английский
Citations
5
International Journal of COPD, Journal Year: 2023, Volume and Issue: Volume 18, P. 2417 - 2429
Published: Nov. 1, 2023
Purpose: Chronic obstructive pulmonary disease (COPD) is the main cause of mortality world widely. Non-coding RNAs (lncRNAs) and associated competitive endogenous (ceRNAs) networks were recently proved to lead mRNA gene expression downregulation but still unclear in COPD. This study aims investigate elucidate mechanisms underlying involvement ceRNA co-expression COPD pathogenesis. Methods: Obtained signature data from Gene Expression Omnibus database compared differentially mRNAs miRNAs between patients healthy smokers. Predicted miRNA–lncRNA miRNA–mRNA interaction using online library employed CIBERSORT measure proportions 22 immune cells control groups. Results: Established a ceRNA-network comprising 11 lncRNAs, 5 miRNAs, 16 mRNAs. Using weighted correlation network analysis method, we identified hub genes obtained one core sub-network, XIST, FGD5-AS1, KCNQ1OT1, HOXA11-AS, LINC00667, H19, PRKCQ-AS1, NUTM2A-AS1/has-mir-454-3p/ZNF678, PRRG4. had different than controls, these variations with magnitude function parameters. Conclusion: The ceRNA-network, particularly may be putative goal for COPD, which specific involved. Keywords: chronic disease, competing RNA network, microRNAs, algorithm, omnibus
Language: Английский
Citations
2
Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)
Published: Jan. 19, 2024
Abstract Segmental instillation of lipopolysaccharide (LPS) by bronchoscopy safely induces transient airway inflammation in human lungs. This model enables investigation pulmonary inflammatory mechanisms as well pharmacodynamic analysis investigational drugs. The aim this work was to describe the transcriptomic profile segmental LPS challenge with contextualization major respiratory diseases. Pre-challenge bronchoalveolar lavage (BAL) fluid and biopsies were sampled from 28 smoking, healthy participants, followed saline control. Twenty-four hours post instillation, BAL collected challenged lung segments. Total RNA cells biopsy samples sequenced analysed for differentially expressed genes (DEGs). After compared saline, 6316 DEGs upregulated 241 downregulated BAL, but only one DEG samples. Upregulated related molecular functions such “ Inflammatory response” or chemokine receptor activity” , pro-inflammatory pathways Wnt-"/“Ras-"/“JAK-STAT” “-signaling pathway” . Furthermore, resembled aspects five most prevalent diseases chronic obstructive disease (COPD), asthma, pneumonia, tuberculosis cancer featured similarities acute exacerbations COPD (AECOPD) community-acquired pneumonia. Overall, our study provides extensive information about mucosal following smokers. It expands knowledge providing potential overlap general infection-triggered insults AECOPD particular.
Language: Английский
Citations
0
Journal of Inflammation Research, Journal Year: 2024, Volume and Issue: Volume 17, P. 3187 - 3200
Published: May 1, 2024
Background: Natural killer (NK) cells are key regulators of immune defense in sepsis-induced acute respiratory distress syndrome (ARDS), yet the characteristics NK cell clusters ARDS remain poorly understood. Methods: A prospective and observational study enrolled septic patients with or not was conducted to determine percentage via flow cytometry. The transcriptomes peripheral blood mononuclear (PBMCs) from healthy controls, sepsis only, were profiled. Vitro experiments performed confirm mechanism mediating MX1 + infiltration. Results: total 115 analyzed, among whom 63 developed 52 did not. Decreased percentages found (%, 7.46± 4.40 vs 11.65± 6.88, P=0.0001) compared sepsis-only patients. lower showed a significant increase 28-day mortality. Single-cell sequencing analysis revealed distinct ARDS, notably identification unique cluster defined as cells. Flow cytometry an elevated specifically individuals only. Pseudo-time that characterized by upregulation type I interferon-induced genes other pro-inflammatory genes. can respond interferons secrete themselves. Ligand–receptor interaction also extensive between T/B cells, leading uncontrolled inflammatory response ARDS. Conclusion: themselves, promoting development Interfering infiltration could be therapeutic approach for this disease. Due limited sample size, larger size needed further exploration. Keywords: inflammation, natural
Language: Английский
Citations
0
The Journal of Headache and Pain, Journal Year: 2024, Volume and Issue: 25(1)
Published: Oct. 1, 2024
Patients with migraine are typically advised to avoid passive smoking because it may aggravate headaches and other health conditions. However, there is insufficient high-quality evidence on the association between migraine, which warrants further investigation using animal models. Therefore, a mouse model, we examined effect of susceptibility cortical spreading depolarization (CSD), biological basis aura.
Language: Английский
Citations
0
World Allergy Organization Journal, Journal Year: 2024, Volume and Issue: 17(12), P. 100995 - 100995
Published: Nov. 21, 2024
Language: Английский
Citations
0