Endocytosis inhibitors block SARS-CoV-2 pseudoparticle infection of mink lung epithelium DOI Creative Commons

Ann Song,

Rattapol Phandthong,

Prue Talbot

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Nov. 14, 2023

Introduction Both spill over and back of SARS-CoV-2 virus have been reported on mink farms in Europe the United States. Zoonosis is a public health concern as dangerous mutated forms could be introduced into human population through spillback. Methods The purpose our study was to determine entry mechanism using lung epithelial cell line (Mv1Lu) block with drug inhibitors. Results Mv1Lu cells were susceptible viral pseudoparticle infection, validating them suitable disease model for COVID-19. Inhibitors TMPRSS2 endocytosis, two pathways entry, tested identify those that blocked infection. inhibitors had minimal impact, which can explained by apparent lack activity this enzyme its localization within cell, not surface. Discussion Dyngo4a, small molecule endocytosis inhibitor, significantly reduced supporting conclusion occurs primarily endocytosis. effective potentially used therapeutically prevent infection populations. This will facilitate development therapeutics zoonotic transmission variants other animals, including humans.

Language: Английский

Smoking and vaping alter genes related to mechanisms of SARS-CoV-2 susceptibility and severity DOI Creative Commons

Rachel Bowsher,

Timothy H. Marczylo,

Karen E. Gooch

et al.

European Respiratory Journal, Journal Year: 2024, Volume and Issue: 64(1), P. 2400133 - 2400133

Published: July 1, 2024

Background Evidence for the impact of smoking on coronavirus disease 2019 (COVID-19) is contradictory, and there little research vaping. Here we provide greater clarity mechanisms perturbed by tobacco cigarette, electronic cigarette nicotine exposures that may risks infection and/or severity. Methods Following Preferred Reporting Items Systematic Reviews Meta-Analyses (PRISMA) guidelines, Ovid Web Science databases were searched. Study design exposure-induced gene expression changes extracted. Each study was quality assessed higher confidence scores assigned to genes consistently changed across multiple studies following same exposure. These used explore pathways significantly altered Results 125 provided data 480 exposure cigarettes, e-cigarettes, or severe acute respiratory syndrome 2 (SARS-CoV-2). Genes involved in both SARS-CoV-2 viral-entry inflammation Pathway analysis revealed many those with high are common cellular processes relating hyperinflammatory immune responses. Conclusion Exposure e-cigarettes therefore initial host–pathogen interactions Smokers vapers could potentially be at increased risk infection, associated cytokine storm, distress syndrome. However, further required, particularly determine biological perturbation subsequent responses within tract. This will improve our physiological understanding vaping COVID-19, informing public health advice providing improved guidance management other viruses.

Language: Английский

Citations

4
E-cigarettes are not associated with post-acute COVID-19 syndrome among US adults DOI Creative Commons
Shahryar Rajai Firouzabadi, Ida Mohammadi,

Mohammadreza Alinejadfard

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 22, 2025

Language: Английский

Citations

0
Contextualizing Changes in e-Cigarette Use During the Early COVID-19 Pandemic and Accompanying Infodemic (“So Much Contradictory Evidence”): Qualitative Document Analysis of Reddit Forums DOI Creative Commons
Shannon Lea Watkins,

Katherine Snodgrass,

Lexi Fahrion

et al.

Journal of Medical Internet Research, Journal Year: 2025, Volume and Issue: 27, P. e66010 - e66010

Published: March 20, 2025

Background Understanding how social media platforms facilitate information exchange and influence behavior during health crises can enhance public responses times of uncertainty. While some risk factors for COVID-19 susceptibility severity (eg, old age) were clear, whether e-cigarette use increased was not clear. People who used e-cigarettes had to navigate both the infodemic a conflicting, politicized, changing environment about interaction between use. Objective This study aims characterize contextualize e-cigarette–related changes early pandemic illuminate role that played in decision-making. Methods We conducted qualitative analysis COVID-19–related discussions on 3 Reddit forums e-cigarettes. collected 189 relevant discussion threads made first 6 months (collected from June 27, 2020, July 3, 2020). Threads included 3155 total comments (mean 17 comments) approximately 1200 unique Redditors. developed applied emergent codes related perceptions behaviors nicotine do-it-yourself narratives) web-based community interactions advice), identified thematic patterns across codes, model synthesize socioecological context behaviors. Results e-Cigarette subreddits provided platform Redditors discuss experiences with e-cigarettes, make sense information, provide emotional support. Discussions reflected an array behavioral responses, including increases decreases intensity, purchasing practices stockpiling), vaping reusing disposable pods). presented theoretically empirically informed circumstances created by activity space product shortages) compelled changes. drew their existing perceptions, intentions, tobacco products; personal experiences; participation decide change pandemic. Forums uncertainty, stress, debate rapidly evolving complicated information. Consumption news articles peer-reviewed publications) Decisions distrust media. Conclusions Variations individual traits environmental why there no unified direction this period. Information also decisions Social is effective important place communicate particularly crisis or disaster situations. Moving forward, transparent, accurate, specific message development should consider struggles, stigma people address roles mistrust misinformation play decisions.

Language: Английский

Citations

0
Challenges in current inhalable tobacco toxicity assessment models: A narrative review DOI Creative Commons
Thivanka Muthumalage,

Alexandra Noël,

Yasmin Thanavala

et al.

Tobacco Induced Diseases, Journal Year: 2024, Volume and Issue: 22(June), P. 1 - 17

Published: June 10, 2024

1. Glasser AM, Collins L, Pearson JL, et al. Overview of electronic nicotine delivery systems: a systematic review. Am J Prev Med. 2017;52(2):e33-e66. doi:10.1016/j.amepre.2016.10.036 CrossRef Google Scholar

Language: Английский

Citations

2
Dual tobacco smoking, electronic cigarette use and COVID-19 outcomes DOI
Tahlia Grammatopoulos,

Ellana Yeoh,

Nouhad El-Haddad

et al.

European Journal of Internal Medicine, Journal Year: 2024, Volume and Issue: 128, P. 156 - 157

Published: May 28, 2024

Language: Английский

Citations

0
A cross-sectional study on the perceived risk of COVID-19 and its association with the usage patterns of e-cigarettes among adolescents and young adults during the COVID-19 pandemic in Nigeria DOI Creative Commons
Omolola Titilayo Alade, Afolabi Oyapero, Bamidele Olubukola Popoola

et al.

Deleted Journal, Journal Year: 2024, Volume and Issue: 21(1)

Published: Oct. 28, 2024

Language: Английский

Citations

0
Impacto de los nuevos dispositivos de suministro de nicotina en las tendencias de consumo y en la salud DOI
Jane Kelli Jacinto da Costa,

E Otero,

Vanesa Hidalgo Sierra

et al.

Medicina de Familia SEMERGEN, Journal Year: 2024, Volume and Issue: 51(5), P. 102413 - 102413

Published: Dec. 18, 2024

Language: Английский

Citations

0
Contextualizing Changes in e-Cigarette Use During the Early COVID-19 Pandemic and Accompanying Infodemic (“So Much Contradictory Evidence”): Qualitative Document Analysis of Reddit Forums (Preprint) DOI
Shannon Lea Watkins,

Katherine Snodgrass,

Lexi Fahrion

et al.

Published: Sept. 1, 2024

BACKGROUND Understanding how social media platforms facilitate information exchange and influence behavior during health crises can enhance public responses times of uncertainty. While some risk factors for COVID-19 susceptibility severity (eg, old age) were clear, whether e-cigarette use increased was not clear. People who used e-cigarettes had to navigate both the infodemic a conflicting, politicized, changing environment about interaction between use. OBJECTIVE This study aims characterize contextualize e-cigarette–related changes early pandemic illuminate role that played in decision-making. METHODS We conducted qualitative analysis COVID-19–related discussions on 3 Reddit forums e-cigarettes. collected 189 relevant discussion threads made first 6 months (collected from June 27, 2020, July 3, 2020). Threads included 3155 total comments (mean 17 comments) approximately 1200 unique Redditors. developed applied emergent codes related perceptions behaviors nicotine do-it-yourself narratives) web-based community interactions advice), identified thematic patterns across codes, model synthesize socioecological context behaviors. RESULTS e-Cigarette subreddits provided platform Redditors discuss experiences with e-cigarettes, make sense information, provide emotional support. Discussions reflected an array behavioral responses, including increases decreases intensity, purchasing practices stockpiling), vaping reusing disposable pods). presented theoretically empirically informed circumstances created by activity space product shortages) compelled changes. drew their existing perceptions, intentions, tobacco products; personal experiences; participation decide change pandemic. Forums uncertainty, stress, debate rapidly evolving complicated information. Consumption news articles peer-reviewed publications) Decisions distrust media. CONCLUSIONS Variations individual traits environmental why there no unified direction this period. Information also decisions Social is effective important place communicate particularly crisis or disaster situations. Moving forward, transparent, accurate, specific message development should consider struggles, stigma people address roles mistrust misinformation play decisions.

Language: Английский

Citations

0
Endocytosis inhibitors block SARS-CoV-2 pseudoparticle infection of mink lung epithelium DOI Creative Commons

Ann Song,

Rattapol Phandthong,

Prue Talbot

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Nov. 14, 2023

Introduction Both spill over and back of SARS-CoV-2 virus have been reported on mink farms in Europe the United States. Zoonosis is a public health concern as dangerous mutated forms could be introduced into human population through spillback. Methods The purpose our study was to determine entry mechanism using lung epithelial cell line (Mv1Lu) block with drug inhibitors. Results Mv1Lu cells were susceptible viral pseudoparticle infection, validating them suitable disease model for COVID-19. Inhibitors TMPRSS2 endocytosis, two pathways entry, tested identify those that blocked infection. inhibitors had minimal impact, which can explained by apparent lack activity this enzyme its localization within cell, not surface. Discussion Dyngo4a, small molecule endocytosis inhibitor, significantly reduced supporting conclusion occurs primarily endocytosis. effective potentially used therapeutically prevent infection populations. This will facilitate development therapeutics zoonotic transmission variants other animals, including humans.

Language: Английский

Citations

0