Gas Therapy: Generating, Delivery, and Biomedical Applications

Small Methods, Journal Year: 2024, Volume and Issue: 8(8)

Published: Jan. 9, 2024

Abstract Oxygen (O 2 ), nitric oxide (NO), carbon monoxide (CO), hydrogen sulfide (H S), and ) with direct effects, dioxide (CO complementary effects on the condition of various diseases are known as therapeutic gases. The targeted delivery in situ generation these gases controllable release at site disease has attracted attention to avoid risk gas poisoning improve their performance treating such cancer therapy, cardiovascular bone tissue engineering, wound healing. Stimuli‐responsive gas‐generating sources systems based biomaterials that enable on‐demand promising approaches for precise therapy. This work highlights current advances design development new generate deliver behavior. delivered biomedical applications is then discussed.

Language: Английский

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Catalase as a sulfide-sulfur oxido-reductase: An ancient (and modern?) regulator of reactive sulfur species (RSS)

Redox Biology, Journal Year: 2017, Volume and Issue: 12, P. 325 - 339

Published: March 1, 2017

Catalase is well-known as an antioxidant dismutating H2O2 to O2 and H2O. However, catalases evolved when metabolism was largely sulfur-based, long before reactive oxygen species (ROS) became abundant, suggesting catalase metabolizes sulfide (RSS). Here we examine of H2Sn, the sulfur analog H2O2, hydrogen (H2S) other sulfur-bearing molecules using H2S-specific amperometric electrodes fluorophores measure polysulfides (H2Sn; SSP4) ROS (dichlorofluorescein, DCF). eliminated but did not anaerobically generate H2S, expected product dismutation. Instead, concentration- oxygen-dependently metabolized H2S in so doing acted a oxidase with P50 20 mmHg. had little effect on catalase-mediated presence inhibitor, sodium azide (Az), rapidly efficiently expedited both normoxia hypoxia effective electron acceptor this reaction. Unexpectedly, concentration-dependently generated from dithiothreitol (DTT) hypoxia, concomitantly oxidizing O2. production DTT inhibited by carbon monoxide augmented NADPH that heme-iron catalytic site provides reducing equivalents. also garlic oil, diallyltrisulfide, thioredoxin dioxide, sulfite, metabisulfite, carbonyl sulfide, cysteine, cystine, glutathione or oxidized glutathione. Oxidase activity present Aspergillus niger. These results show can act either reductase they suggest these activities likely played prominent role during evolution may continue do modern cells well. This appears be first observation independent peroxide

Language: Английский

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Oxygen sensing and signal transduction in hypoxic pulmonary vasoconstriction

European Respiratory Journal, Journal Year: 2015, Volume and Issue: 47(1), P. 288 - 303

Published: Oct. 22, 2015

Hypoxic pulmonary vasoconstriction (HPV), also known as the von Euler-Liljestrand mechanism, is an essential response of vasculature to acute and sustained alveolar hypoxia. During local hypoxia, HPV matches perfusion ventilation maintain optimal arterial oxygenation. In contrast, during global leads hypertension. The oxygen sensing signal transduction machinery located in smooth muscle cells (PASMCs) pre-capillary vessels, albeit physiological may be modulated vivo by endothelium. While factors such nitric oxide modulate HPV, reactive species (ROS) have been suggested act mediators HPV. ROS originate from mitochondria and/or NADPH oxidases but exact mechanisms, well question whether increased or decreased cause are under debate. induce intracellular calcium increase subsequent contraction PASMCs via direct indirect interactions with protein kinases, phospholipases, sarcoplasmic channels, transient receptor potential voltage-dependent potassium channels L-type whose relevance vary different experimental conditions. Successful identification regulating allow development novel therapeutic approaches for conditions disturbed

Language: Английский

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Hydrogen sulfide in pharmacology and medicine – An update

Pharmacological Reports, Journal Year: 2015, Volume and Issue: 67(3), P. 647 - 658

Published: Jan. 22, 2015

Language: Английский

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Hydrogen Sulfide as an Oxygen Sensor

Antioxidants and Redox Signaling, Journal Year: 2014, Volume and Issue: 22(5), P. 377 - 397

Published: May 7, 2014

Although oxygen (O2)-sensing cells and tissues have been known for decades, the identity of O2-sensing mechanism has remained elusive. Evidence is accumulating that O2-dependent metabolism hydrogen sulfide (H2S) this enigmatic O2 sensor.The elucidation biochemical pathways involved in H2S synthesis shown reciprocal H2S/O2 interactions inexorably linked throughout eukaryotic evolution; there are multiple foci by which controls inactivation, effects on downstream signaling events consistent with those activated hypoxia. H2S-mediated sensing demonstrated a variety vertebrate cardiovascular respiratory systems, including smooth muscle systemic blood vessels airways, carotid body, adrenal medulla, other peripheral as well central chemoreceptors.Information now needed intracellular location stoichometry these processes how effectors its metabolites.Development specific inhibitors effector activation cellular organelle-targeted compounds release time- or environmentally controlled way will not only enhance our understanding process but also provide direction future therapeutic applications.

Language: Английский

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H2S and polysulfide metabolism: Conventional and unconventional pathways

Biochemical Pharmacology, Journal Year: 2017, Volume and Issue: 149, P. 77 - 90

Published: Dec. 14, 2017

Language: Английский

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Mitochondria, the gut microbiome and ROS

Cellular Signalling, Journal Year: 2020, Volume and Issue: 75, P. 109737 - 109737

Published: Aug. 15, 2020

Language: Английский

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Exogenous administration of thiosulfate, a donor of hydrogen sulfide, attenuates angiotensin II‐induced hypertensive heart disease in rats

British Journal of Pharmacology, Journal Year: 2014, Volume and Issue: 172(6), P. 1494 - 1504

Published: June 24, 2014

Background and Purpose Hypertension is an important mediator of cardiac damage remodelling. Hydrogen sulfide ( H 2 S ) endogenously produced gasotransmitter with cardioprotective properties. However, it not yet in clinical use. We, therefore, investigated the protective effects sodium thiosulfate STS ), a clinically applicable donor substance, angiotensin II A ng )‐induced hypertensive disease rats. Experimental Approach M ale prague D awley rats were infused (435 kg min −1 or saline (control) for 3 weeks via s.c. placed osmotic minipumps. During these weeks, received i.p. injections either , NaHS vehicle (0.9% NaCl ). Key Results Compared controls, infusion caused increase systolic diastolic BP associated as evidenced by hypertrophy, atrial natriuretic peptide ANP m RNA fibrosis increased oxidative stress. Treatment prevented development hypertension levels. Furthermore, degree extent histological combination expression profibrotic genes levels stress all significantly decreased. Conclusions Implications ‐induced can be attenuated treatment . Although regulation most plausible mechanism protection, antifibrotic antioxidant properties released may also contribute to their effects. Our data show that might valuable addition already existing antihypertensive therapies. Linked Articles This article part themed section on Pharmacology Gasotransmitters. To view other articles this visit http://dx.doi.org/10.1111/bph.2015.172.issue‐6

Language: Английский

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Beyond a Gasotransmitter: Hydrogen Sulfide and Polysulfide in Cardiovascular Health and Immune Response

Antioxidants and Redox Signaling, Journal Year: 2017, Volume and Issue: 27(10), P. 634 - 653

Published: April 11, 2017

Significance: Hydrogen sulfide (H2S) metabolism leads to the formation of oxidized species, including polysulfide, persulfide, and others. Evidence is emerging that many biological effects H2S may indeed be due polysulfide persulfide activation signaling pathways reactivity with discrete small molecules. Recent Advances: Exogenous polysulfides, are more reactive than a wide range Importantly, endogenous has been reported occur via transsulfuration enzymes, cystathionine γ-lyase (CSE) β-synthase (CBS). Critical Issues: In light recent understanding metabolite reactivity, comparatively few studies have comparing cellular in vivo donors versus donors. Likewise, it equally unclear when, how, what extent occurs under pathophysiological conditions. Future Directions: Additional regarding molecular reactions needed nearly all aspects biology better understand how metabolites contribute key chemical involved cardiovascular health immune responses. Antioxid. Redox Signal. 27, 634–653.

Language: Английский

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Hydrogen sulfide attenuates calcification of vascular smooth muscle cells via KEAP1/NRF2/NQO1 activation

Atherosclerosis, Journal Year: 2017, Volume and Issue: 265, P. 78 - 86

Published: Aug. 19, 2017

Language: Английский

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