Transcriptomic and lipidomic profiling reveals distinct bioactive lipid signatures in slow and fast muscles and highlights the role of resolvin‐D2 in fiber type determination during myogenesis DOI Creative Commons

Lupann Rieger,

Thomas Molina, Paul Fabre

et al.

The FASEB Journal, Journal Year: 2024, Volume and Issue: 38(24)

Published: Dec. 13, 2024

Skeletal muscles are predominantly composed of long, multinucleated muscle fibers, classified according to their metabolic and contractile phenotype. The determination fiber types is influenced by various factors (e.g., innervation, hormones, physical demand). Our laboratory others showed that resolvins, lipid mediators derived from omega-3 fatty acids, promote regeneration function after an injury or in models muscular dystrophies; however, the effect resolvins on phenotype remains unknown. Here, we investigated impact during myogenesis. Transcriptomics analysis single-nuclei RNAseq data sets revealed enzymes responsible for bioactive lipids biosynthesis differentially expressed slow fibers versus fast fibers. Lipidomics slow-twitch (soleus) fast-twitch (tibialis anterior) levels arachidonic acid similar between groups, but alpha-linolenic acid, linoleic eicosapentaenoic docosahexaenoic enriched muscle. Screening different vitro resolvin-D2 enhances formation myotubes expressing myosin heavy chain isoform. In vivo, administration strength, increases myofiber size, affects typing injured not uninjured muscles. Resolvin-D2 promoted transition toward dominant regenerating (i.e., type I soleus IIB tibialis anterior muscle), suggesting its participation conjunction with other factors. Overall, these findings identified new roles regulation typing, which could have therapeutic applicability injuries dystrophies.

Language: Английский

Human skeletal muscle fiber heterogeneity beyond myosin heavy chains DOI Creative Commons
Roger Moreno‐Justicia, Thibaux Van der Stede, Ben Stocks

et al.

Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 19, 2025

Skeletal muscle is a heterogenous tissue comprised primarily of myofibers, commonly classified into three fiber types in humans: one "slow" (type 1) and two "fast" 2A type 2X). However, heterogeneity between within traditional remains underexplored. We applied transcriptomic proteomic workflows to 1050 1038 single myofibers from human vastus lateralis, respectively. Proteomics was conducted males, while transcriptomics included ten males females. identify metabolic, ribosomal, cell junction proteins, addition myosin heavy chain isoforms, as sources multi-dimensional variation myofibers. Furthermore, whilst slow fast clusters are identified, our data suggests that 2X fibers not phenotypically distinct other fibers. Moreover, chain-based classifications do adequately describe the phenotype nemaline myopathy. Overall, indicates myofiber with beyond isoforms. Moreno-Justicia colleagues report pipelines explore skeletal fibers, identifying healthy individuals children

Language: Английский

Citations

2
Molecular aspects of the exercise response and training adaptation in skeletal muscle DOI Creative Commons
Regula Furrer, Christoph Handschin

Free Radical Biology and Medicine, Journal Year: 2024, Volume and Issue: 223, P. 53 - 68

Published: July 24, 2024

Skeletal muscle plasticity enables an enormous potential to adapt various internal and external stimuli perturbations. Most notably, changes in contractile activity evoke a massive remodeling of biochemical, metabolic force-generating properties. In recent years, large number signals, sensors, regulators effectors have been implicated these adaptive processes. Nevertheless, our understanding the molecular underpinnings training adaptation remains rudimentary. Specifically, mechanisms that underlie signal integration, output coordination, functional redundancy other complex traits are unknown. fact, it is even unclear how stimulus-dependent specification brought about endurance or resistance exercise. this review, we will provide overview on events describe acute perturbations single exercise bouts. Furthermore, insights into principles long-term adaptation. Finally, current gaps knowledge be identified, strategies for multi-omic –cellular analyses skeletal engaged individual, bouts chronic discussed.

Language: Английский

Citations

7
Techniques, Models, and Variables of Interest in Studies of Intrinsic Laryngeal Muscle Fiber Types: A Bibliometric Analysis DOI
Christopher S Apfelbach, Lady Catherine Cantor-Cutiva, Adrián Castillo‐Allendes

et al.

Journal of Voice, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Optimizing Confocal Imaging Protocols for Muscle Fiber Typing in the Mouse Masseter Muscle DOI Creative Commons

Catalina Matias,

Chiaki Yamada, Alexandru Movila

et al.

BIO-PROTOCOL, Journal Year: 2025, Volume and Issue: 15(1368)

Published: Jan. 1, 2025

The masseter muscle, a key orofacial demonstrates unique anatomical and functional properties, including sexual dimorphism in myosin heavy chain (MyHC) expression complex fiber architecture. Despite its importance mastication relevance to various disorders, phenotypic characterization of the remains limited. Conventional fluorescence microscopy has been cornerstone muscle typing, reliably identifying MyHC isoforms measuring cross-sectional areas. Building on this foundation, confocal offers complementary advantages, such as enhanced resolution, increased flexibility for multiplexing, ability visualize structures three dimensions. This study presents detailed protocol using achieve high-resolution imaging molecular cryosections. By leveraging advanced technologies white light lasers extended z-length imaging, method ensures precise spectral separation, simultaneous multichannel detection, capture architecture includes tissue preparation, immunostaining isoforms, postprocessing segmentation quantification. setup was optimized minimizing signal bleed through, improving signal-to-noise ratio, enabling visualization fibers markers. Image allows quantification area individual fibers, nuclei location measurements, identification within each fiber. microscopy-based provides similar resolution contrast compared conventional techniques, robust multiplexed 3D reconstruction structures. These advantages make it valuable tool studying architecture, offering broad applications physiology pathology research. Key features • Enables capturing spatial relationships separation techniques. Supports detection multiple markers typing localization. Allows generation three-dimensional models analyze neuromuscular junctions, extracellular matrix, mitochondrial organization. Adaptable skeletal muscles species, providing insights into physiology, regeneration, disease processes. Graphical overview Analyzing composition morphology mice's microscopy. Workflow characterizing rodent Confocal microscopy, equipped with laser technology z-stack unmixing reduce through enhance detection. is beyond physical thickness sample account potential variations flatness ensure complete all focal planes. resulting images provide composition, areas, ensuring reproducible data analyzing characteristics other muscles.

Language: Английский

Citations

0
Comment on “Fiber-type traps: revisiting common misconceptions about skeletal muscle fiber types with application to motor control, biomechanics, physiology, and biology” DOI
Carlo Reggiani, Marta Murgia

Journal of Applied Physiology, Journal Year: 2024, Volume and Issue: 136(2), P. 437 - 438

Published: Feb. 1, 2024

Language: Английский

Citations

1
Functional pain syndromes in the context of functional neurological disorders DOI
Michael Brinkers,

K. Niemier

Manuelle Medizin, Journal Year: 2024, Volume and Issue: 62(2), P. 95 - 101

Published: April 9, 2024

Citations

1
High-intensity interval training using electrical stimulation ameliorates muscle fatigue in chronic kidney disease-related cachexia by restoring mitochondrial respiratory dysfunction DOI Creative Commons
Hiroyori Fusagawa,

Tatsuya Sato,

Takashi Yamada

et al.

Frontiers in Physiology, Journal Year: 2024, Volume and Issue: 15

Published: June 26, 2024

Background Exercise, especially high-intensity interval training (HIIT), can increase mitochondrial respiratory capacity and enhance muscular endurance, but its systemic burden makes it difficult to safely continuously prescribe for patients with chronic kidney disease (CKD)-related cachexia who are in poor general condition. In this study, we examined whether HIIT using electrical stimulation (ES), which does not require whole-body exercise, improves muscle endurance the skeletal of 5/6 nephrectomized rats, a widely used animal model CKD-related cachexia. Methods Male Wistar rats (10 weeks old) were randomly assigned group sham-operated (Sham) nephrectomy (Nx) rats. was performed on plantar flexor muscles vivo supramaximal ES every other day 4 assess myosin heavy-chain isoforms, function Nx A single session also identify upstream signaling pathways altered by ES. Results non-trained from significantly lower than that Sham The proportion heavy chain IIa/x, content, capacity, formation supercomplexes plantaris decreased compared those Treatment improved these molecular functional changes same degrees as Furthermore, increased phosphorylation levels AMP-activated protein kinase (AMPK) p38 mitogen-activated (MAPK), essential activation both Conclusion findings suggest ameliorates fatigue via restoration dysfunction AMPK MAPK signaling. Our ES-based protocol be without placing whole body promising intervention is implemented even conditions reduced performance status such

Language: Английский

Citations

1
Effects of age on human skeletal muscle: A systematic review and meta-analysis of myosin heavy chain isoform protein expression, fiber size and distribution DOI
Christopher Lee, Philip C. Woods, Amanda E. Paluch

et al.

AJP Cell Physiology, Journal Year: 2024, Volume and Issue: 327(6), P. C1400 - C1415

Published: Oct. 7, 2024

Human studies examining the cellular mechanisms behind sarcopenia, or age-related loss of skeletal muscle mass and function, have produced inconsistent results. A systematic review meta-analysis were performed to determine aging effects on protein expression, size, distribution fibers with various myosin heavy chain (MyHC) isoforms. Study eligibility included MyHC comparisons between young (18–49 yr) older (≥60 adults, 27 identified. Relative expression was higher age for slow-contracting I fibers, correspondingly lower fast-contracting II IIA values. Fiber sizes similar I, but smaller IIA. distributions age. When separated by sex, few that examined females showed atrophy age, no change in expression. Additional analyses measurement technique, physical activity, biopsies provided important insights. In summary, lead more slow-contracting, force-producing isoform male muscles, which helps explain their whole force, velocity, power. Exercise pharmacological interventions shift toward faster isoforms and/or increase fiber size should decrease prevalence sarcopenia. Our findings also indicate future need include focus solely females, measure IIX separately, examine type distribution, sample additional muscles vastus lateralis (VL), incorporate an objective activity.

Language: Английский

Citations

1
Human Skeletal Muscle Fiber Heterogeneity Beyond Myosin Heavy Chains DOI Open Access
Roger Moreno‐Justicia, Thibaux Van der Stede, Ben Stocks

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Sept. 12, 2023

Abstract Skeletal muscle is an inherently heterogenous tissue comprised primarily of myofibers, which are historically classified into three distinct fiber types in humans: one “slow” (type 1) and two “fast” 2A type 2X), delineated by the expression myosin heavy chain isoforms (MYHs). However, heterogeneity between within traditional remains underexplored. Indeed, whether MYHs main classifiers skeletal fibers has not been examined unbiased manner. Through development application novel transcriptomic proteomic workflows, applied to 1050 1038 single from human vastus lateralis , respectively, we show that only principal drivers heterogeneity. Instead, metabolic, ribosomal, cell junction proteins a source multi-dimensional variation fibers. Furthermore, whilst slow fast clusters can be identified, described their contractile metabolic profiles, our data suggests 2X phenotypically other at omics level. Moreover, MYH-based classifications do adequately describe phenotype most common genetic diseases, nemaline myopathy, with shifting towards non-oxidative independently type. We also characterize features fibers, including identifying several type-specific polypeptides, termed microproteins, encoded transcripts annotated as non-coding RNA. Overall, indicates sources beyond isoforms.

Language: Английский

Citations

2
Reply to Drs. Reggiani and Murgia DOI
Silvia S. Blemker, Susan V. Brooks, Karyn A. Esser

et al.

Journal of Applied Physiology, Journal Year: 2024, Volume and Issue: 136(2), P. 439 - 439

Published: Feb. 1, 2024

Language: Английский

Citations

0