Time-lapse imaging of identified granule cells in the mouse dentate gyrus after entorhinal lesion in vitro reveals heterogeneous cellular responses to denervation DOI Creative Commons

Davide Greco,

Alexander Drakew,

Nina Rößler

et al.

Frontiers in Neuroanatomy, Journal Year: 2025, Volume and Issue: 18

Published: Jan. 21, 2025

Denervation of neurons is a network consequence brain injury. The effects denervation on can be readily studied in vitro using organotypic slice cultures entorhinal cortex and hippocampus. Following transection the entorhino-dentate projection, granule cells (GCs) are denervated show average transient loss spines their distal dendrites but not non-denervated proximal dendrites. In present study, we addressed question how single GCs segments react to denervation. Local adeno-associated virus (AAV)-injections were employed transduce dentate with tdTomato projection EGFP. This made it possible visualize both innervating fibers target identify dendritic located “entorhinal” “hippocampal” zone gyrus. Confocal time-lapse imaging was used image after Time-matched served as controls. line previous reports, spine ~30% (2–4 days post-lesion) zone. However, individual showed considerable variability response layers, decreases well increases density observed at cell level. Based standard deviations effect sizes this computer simulation yielded recommendations for minimum number that should analyzed future studies model.

Language: Английский

Time-lapse imaging of identified granule cells in the mouse dentate gyrus after entorhinal lesion in vitro reveals heterogeneous cellular responses to denervation DOI Creative Commons

Davide Greco,

Alexander Drakew,

Nina Rößler

et al.

Frontiers in Neuroanatomy, Journal Year: 2025, Volume and Issue: 18

Published: Jan. 21, 2025

Denervation of neurons is a network consequence brain injury. The effects denervation on can be readily studied in vitro using organotypic slice cultures entorhinal cortex and hippocampus. Following transection the entorhino-dentate projection, granule cells (GCs) are denervated show average transient loss spines their distal dendrites but not non-denervated proximal dendrites. In present study, we addressed question how single GCs segments react to denervation. Local adeno-associated virus (AAV)-injections were employed transduce dentate with tdTomato projection EGFP. This made it possible visualize both innervating fibers target identify dendritic located “entorhinal” “hippocampal” zone gyrus. Confocal time-lapse imaging was used image after Time-matched served as controls. line previous reports, spine ~30% (2–4 days post-lesion) zone. However, individual showed considerable variability response layers, decreases well increases density observed at cell level. Based standard deviations effect sizes this computer simulation yielded recommendations for minimum number that should analyzed future studies model.

Language: Английский

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